Quenching + FRET

Quenching and Lifetime of FluorophoresStatic quenching will not reduce the lifetime of the sample since those fluorophoreswhich are not complexed – and hence are able to emit after excitation – will havenormal excited state properties. The fluorescence of the sample is reduced sincethe quencher is essentially reducing the number of fluorophores which can emit.(1) Dynamic QuenchingF 0/F = 1 + k qt o[Q]t 0/t = 1 + k qt o[Q]F o / Ft o / t[Q][Q](2) Static QuenchingF 0/F = 1 + K a[Q]t 0= tF o / Ft o / t[Q][Q]