Prostaglandin E2 Metabolite ELISA - IBL International

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Prostaglandin E Metabolite EIA Kit Catalog No. 514531 (Strip Plate) Catalog No. 514531.1 (Solid Plate)
TABLE OF CONTENTS GENERAL INFORMATION 3 Materials Supplied 4 Precautions 4 If You Have Problems 4 Storage and Stability INTRODUCTION 5 Background 4 Materials Needed but Not Supplied 5 About This Assay 7 Description of ACE TM Competitive EIAs 8 Biochemistry of Acetylcholinesterase 10 Definition of Key Terms PRE-ASSAY PREPARATION 11 Buffer Preparation 12 Sample Preparation 13 Sample Purification ASSAY PROTOCOL 17 Derivatization of Standards and Samples to PGEM 19 Preparation of Assay-Specific Reagents 21 Plate Set Up 22 Performing the Assay ANALYSIS 25 Calculations 28 Performance Characteristics RESOURCES 32 Troubleshooting 32 Additional Reading 33 References 33 Related Products 34 Warranty and Limitation of Remedy 35 Plate Template 36 Notes Materials Supplied GENERAL INFORMATION Catalog Number Item 96 wells Quantity/Size GENERAL INFORMATION 480 wells Quantity/Size 414532 Prostaglandin E Metabolite EIA Antiserum 1 vial/100 dtn 1 vial/500 dtn 414530 Prostaglandin E Metabolite AChE Tracer 1 vial/100 dtn 1 vial/500 dtn 414534 Prostaglandin E Metabolite EIA Standard 1 vial 1 vial 400060 EIA Buffer Concentrate (10X) 2 vials/10 ml 4 vials/10 ml 400062 Wash Buffer Concentrate (400X) 1 vial/5 ml 1 vial/12.5 ml 400035 Tween 20 1 vial/3 ml 1 vial/3 ml 400005 Mouse Anti-rabbit IgG Coated Plate 1 plate 5 plates 400012 Plate Cover 1 cover 5 covers 400050 Ellman’s Reagent 3 vials/100 dtn 6 vials/250 dtn 400032 Phosphate Buffer 1 vial/100 dtn 2 vials/250 dtn 400027 Carbonate Buffer 1 vial/100 dtn 1 vial/500 dtn 400040 EIA Tracer Dye 1 vial 1 vial 400042 EIA Antiserum Dye 1 vial 1 vial If any of the items listed above are damaged or missing, please contact our Customer Service department at (800) 364-9897 or (734) 975-3999. We cannot accept any returns without prior authorization. ! WARNING: Not for human or animal disease diagnosis or therapeutic drug use. 3
Page 1: Prostaglandin E2 Metabolite ELISA E
Page 5 and 6: HO t 1/2 - 3 minutes in plasma 15-O
Page 7 and 8: Definition of Key Terms Blank: back
Page 9 and 10: C-18 SPE Purification Protocol Mate
Page 11 and 12: Preparing the Standard Curve NOTE:
Page 13 and 14: Performing the Assay Pipetting Hint
Page 15 and 16: Plot the Standard Curve Plot %B/B 0
Page 17 and 18: Precision: The intra- and inter-ass
Page 19 and 20: Warranty and Limitation of Remedy C
Page 21: Symbols / Symbole / Symbôles / Sí

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