DNA Analysis - Yeast Cells by PI - Stowers Institute for Medical ...

STANDARD OPERATING PROCEDURE Page 1 of 4CYTO.ADMIN.001.00TITLE:COMPOSING A STANDARD OPERATING PROCEDURESECTION:CYTOMETRYSOP NUMBER:CYTO.ADMIN.001REVISION: 00EFFECTIVE DATE:ORIGINATED BY: WUNDERLICH, JOSHUAREASON FOR REVISION: NEW SOPAPPROVAL DATE:REVIEWED BY (AA):BRYANT, ROBINNAME SIGNATURE DATEREVIEWED BY:JAYASINGHE, SACHNAME SIGNATURE DATEAPPROVED BY:HAUG, JEFFNAME SIGNATURE DATE1000 East 50 th Street, Kansas City, Missouri 64110Telephone: (816) 926-4108, Fax: (816) 926-2081www.stowers-institute.org

STANDARD OPERATING PROCEDURE Page 2 of 4CYTO.ADMIN.001.00Scope:Purpose:Definitions:Responsibility:Equipment:Procedure:Any Cytometry Facility staff member may write and/or revise a standardoperating procedure (SOP) if it is determined that one is needed, though thistask will primarily be performed by the QA/QC Officer.The purpose of this SOP is to outline the required fields and steps forwriting, reviewing, accepting, and revising a Cytometry Facility StandardOperating Procedure.Revision- A change made to an effective SOP (does not refer to SOPs inprogress)All Cytometry Staff, including, but not limited to Managing Director, LabManager, QA/QC Officer, and Administrative Assistant.NoneUpon the determination of a need for an SOP, the following steps shall betaken:I. The cover page of any SOP must contain the following information:a. Titleb. Sectionc. SOP Numberd. Revisione. Effective Datef. Originated Byg. Reason for Revisionh. Approval Datei. Reviewed Byj. Approved ByII. Any SOP must contain the following fields:a. Scope- Describes who, where and what the SOP applies to.b. Purpose- Describes the purpose of the SOP.c. Definitions- Any unclear terms are defined in this section.d. Responsibility- Clearly defines those responsible for performingthe procedure in question.e. Equipment- Describes any special equipment needed to performthe procedure.f. Procedure- Clearly lists the steps required to complete theprocedure. May also include a flowchart.g. Change Historyi.If this is a new SOP, states “New SOP”ii. If this is a revision, lists approval dates, authors, andreasons for all prior revisions.h. References- Lists any SOP’s to which SOP in question refers.Also includes scientific literature references.1000 East 50 th Street, Kansas City, Missouri 64110Telephone: (816) 926-4108, Fax: (816) 926-2081www.stowers-institute.org

STANDARD OPERATING PROCEDURE Page 3 of 4CYTO.ADMIN.001.00i. Appendices- Lists any other documents to which this SOPapplies.III. After an SOP is written, it must be reviewed by:a. The QA/QC Officer if s/he is not the originator.b. The Lab Manager if the QA/QC Officer is the originator.c. The Administrative Assistant, regardless of the originator.IV. After the reviewer has made comments, the originator must addressthe reviews and repeat step III until no further changes arenecessary.V. After all reviewers’ comments are sufficiently addressed, the SOPmust be submitted for approval to:a. The Managing Director if s/he is not the originator or thereviewer.b. The Lab Manager if the Managing Director is either theoriginator or the reviewer.c. The QA/QC Officer in all other cases.VI. If the SOP is not approved, steps III-V shall be repeated untilapproved.VII. An SOP will not be effective until the date of approval.VIII. All steps apply to the revision of an SOP.1000 East 50 th Street, Kansas City, Missouri 64110Telephone: (816) 926-4108, Fax: (816) 926-2081www.stowers-institute.org

STANDARD OPERATING PROCEDURE Page 4 of 4CYTO.ADMIN.001.00Determine Need for NewSOPCompose SOP Accordingto GuidelinesYSubmit SOP to for ReviewDoesReviewersuggestchanges?NNSubmit SOP to forApprovalIs SOPApproved?YPut SOP into EffectReferences:Appendices:NoneNone.1000 East 50 th Street, Kansas City, Missouri 64110Telephone: (816) 926-4108, Fax: (816) 926-2081www.stowers-institute.org