1 Dar es Salaam, Tanzania - CAHFS, University of Minnesota

1Meeting the challenges for bovine TB at the intersection of human health,animal health and the environmentProject leader – Robert B. Morrison, DVM, MBA, PhD612-625-9276; BobM@UMN.Edu385 1988 Fitch Avenue,St. Paul, MNCo-investigators- College of Veterinary Medicine - Srinand Sreevatsan, Katey Pelican- Infectious Disease / Medicine - William StaufferProject team members and roles:- Infectious Disease / Medicine - David Boulware,- Med School IMER Program Office – Paul Quie- Hennepin County Medical Center - Steve Dunlop- School of Public Health - Bruce Alexander- College Vet Med - Tom Molitor, Scott Wells- Applied Econ - Brian Buhr & Nancy Johnson (ILRI)- Whole Village Project - Kari Hartwig, Craig Packer- UMN Foundation - Jan GerstenbergerTanzanian collaborating partners:- Dr. Sayoki G Mfinanga, MD,PhD, DirectorMuhimbili Medical Research CentreNational Institute for Medical Research (NIMR)Dar es Salaam, Tanzania- Dr. Yongolo MmetaCentral Veterinary LaboratoryDar es Salaam, Tanzania- Dr. Mark JacobsonSelian Lutheran Hospital & Arusha Lutheran Medical CenterArusha, TanzaniaBackground:The concept of “One Health” has become a predominant theme in global health. However, despite majornational and international organizations focusing on this concept of integrated health, there are few, ifany, operational projects that truly demonstrate a model of One Health. Bovine TB is thought to beendemic in Tanzanian villages causing increased morbidity and mortality in humans, cattle and wildlife.The disease is an ideal target for a One Health project due to its presumed high prevalence, impact onhuman health and family income, interests and expertise of our working group, and likelihood ofidentifying funding and achieving programmatic success. This pilot project could have far reachingimpact on the Universities approach to research and development as well as put future projects in goodstanding due to the current focus of funders on this concept.The goals and objectives for the partnership are to:- meet in St Paul, MN and discuss collaborative projects addressing the following issues:1. prevalence of bovine TB in livestock, wildlife and humans,2. role of nutrition, bovine TB, HIV, malaria and particulate matter (household-level airpollution) in the respiratory disease complex.3. role of wildlife in incidence of bovine TB in cattle and humans,

24. best treatment for humans5. an economically viable control program for livestock.Justification of partnership and the synergy offered by the proposed collaborationBovine TB is a disease complex that requires an interdisciplinary approach (Figure 1). Our proposedpartnership has all the individual pieces of knowledge, insight and experience necessary to respond. Inaddition, we have relationships with:- USAID RESPOND,- Savannas Forever and Whole Village Project,- SNV (Dutch aid agency) who is promoting and installing an intervention that decreases respirableparticulate matter,- a TB laboratory in Arusha,- professional training program,- two hospitals with outreach programs in ArushaFigure 1. The complex interactions of bovine in Tanzania.Role inrespiratoryHealth (w/ HIVparticulates, etc)Public HealthRisk factorsMedicinedxtreatmentBovine TBWildlife interactionEconomic impactlivelihoodMarketing productRisk factorsVet MedMolecularcharacterizationDx and preventionDiscussion of the long-range potential of the partnership and potential sources of fundingWe are optimistic that funding will be identified through USAID, McKnight Foundation, GatesFoundation, National Institutes of Health, and USDA-NIFA TB-CAP program. The group is alreadyworking on a grant application to NIH to build diagnostic capacity for bovine tuberculosis.Strategy to achieve the objectives, including the activities, timeline and deliverables.Our initial intent is to meet with our collaborating partners and explore activities, funding opportunitiesand a timeline.What success will look like:At least one funded, interdisciplinary project with the ultimate goal of improving health and livelihood ofTanzanian villagers.An evaluation methodology for measuring outcomes/impact in the context of “success”- funding successfully identified,- joint proposals submitted,- internships, training or exchanges established,- progress in understanding and perhaps reducing complexity of bovine TB, as indicated byrefereed publications.

3BudgetOne HealthTravel3 x $2,500 $7,500Hotel3 x 3 nights x $150 $1,350Meals $1,150Total $10,000

4BIOGRAPHICAL SKETCHNAMERobert B. MorrisonPOSITION TITLEProfessor of Veterinary MedicineBobM@UMN.EduEDUCATION/TRAININGINSTITUTION AND LOCATION DEGREE MM/YY FIELD OF STUDYUniversity of Saskatchewan, Canada BSc 04/74 BiologyUniversity of Saskatchewan, Canada DVM 04/79 Veterinary MedicineUniversity of Minnesota PhD 12/84 EpidemiologyUniversity of Minnesota MBA 06/94 Business administrationPositions & Honors:1979-1981 - Associate veterinarian in multi-person mixed animal practice1981-1984 - Veterinary Medical Assistant/Research Associate1981-1984 - Associate veterinarian in large animal practice, (part-time)1985-1986 - Associate Expert in Epidemiology for FAO of the United Nations1986-1991 - Assistant Professor in swine medicine; U of MN1991-1999 - Associate Professor in swine medicine; U of MN2000-2010 – Professor in swine medicine; U of MN1996 AASV Meritorious service award2005 Certificate for excellence in teaching, UMN CVM2010 AASV Howard Dunne award for service to the professionRecent Publications:1. Kritas SK, Morrison RB. Effect of orally administered Lactobacillus casei on PorcineReproductive and Respiratory Syndrome (PRRS) virus vaccination in pigs. VeterinaryMicrobiol 2007; Vol 114, 248-255.2. Mondaca–Fernández E, Meyns T, Muñoz-Zanzi C, Trincado C, Morrison RB.Experimental quantification of the transmission of Porcine reproductive and respiratorysyndrome virus. Canadian J of Vet Research 2007; 71: 157-160.3. Schaefer N, Morrison RB. Effect on total pigs weaned of herd closure for elimination ofporcine reproductive and respiratory virus. J Swine Health & Production 2007; Vol 15No 3, 152-155.4. Mondaca-Fernández E, Morrison RB. Applying spatial analysis for a PRRSV regionalcontrol program. Vet Rec 2007, vol. 161, n o 4, pp. 137-138.5. Jean Paul Cano, Scott A. Dee, Michael P. Murtaugh, Albert Rovira, Morrison RB.Infection dynamics and clinical manifestations following experimental inoculation ofgilts at 90 days of gestation with porcine reproductive and respiratory syndrome virus.Canadian Journal of Veterinary Research October 2009 Vol 73; No 4, 303-307.

5NAMEWilliam Moyer StauffereRA COMMONS USER NAMEWStaufferBIOGRAPHICAL SKETCHProvide the following information for the key personnel and other significant contributors.Follow this format for each person. DO NOT EXCEED FOUR PAGES.POSITION TITLEAssociate ProfessorEDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)INSTITUTION AND LOCATIONDEGREE(if applicable)YEAR(s)FIELD OF STUDYSt. Olaf College, Minneapolis, MN B.A. 1988University of Colorado, Denver, CO M.S.P.H. 1992 Health SciencesUniversity of Minnesota, Minneapolis, MN M.D. 1995 Medicine and PediatricsB. Recent Positions and Honors:- Technical Advisor. Division of Quarantine and Global Migration. Immigrant and Refugee HealthBranch. Centers for Disease Control and Prevention, Atlanta (2005 – present)- Expert Consultants Roster to the World Health Organization for the Revision of the InternationalHealth Regulations (accordance with Article 47). (June 2009-- present).- President-elect. American Committee on Clinical Tropical Medicine and Travelers Health.American Society of Tropical Medicine and Hygiene (2009/2010)- Infectious Disease Society of America. Committee for the Development of Diagnostic and Treatmentof Parasitic Diseases Guidelines (2009--)- University of Minnesota, Advisory Committee on International Scholarship. 2006-present.- Minnesota Department of Health Tuberculosis Advisory Committee. 2006-present.- Community Partners Recognition. School of Public Health. 2006-present.- Elected position. Data Use and Protection Committee. GESOSENTINAL. 2006-present.C. Recent Peer-reviewed publications or manuscripts in press in chronological order:Stauffer WM, Weinberg M, Newman RD, et al. Presumptive treatment of P. falciparum malariain refugees relocating from sub-Saharan Africa to the United States. Am J Trop Med Hyg2008;79:141-146.Stauffer WM, Kluznik J, Dicker S, Boulware DR, Walker PF. Migrants, detainees andmisconceptions. Lancet 2009;373(9682):2112.Yanni EA, Marano N, Stuaffer WM, Barnett E, Cano M, Cetron M. Health status of visitors andtemporary residents, United States (CDC). Emerg Inf Dis 2009;15(11): 1715-1720.Stauffer WM, Cartwright C, Olson D, Taylor C, Juni B, Bowers S, Hanson K, Rosenblatt J,DeVries A. Superior diagnostic performance of malaria rapid tests as compared to bloodsmears in U.S. clinical practice. Clin Infect Dis. 2009;49;908-913.Baker C, Thomas W, Stauffer WM, et al. Serial testing of refugees for latent tuberculosis usingthe QuantiFERON-Gold In-Tube: Effects of antecedent tuberculin skin test and vitamin Ddeficiency. Am J Trop Med Hyg. 2009;80(4):628-33.Hagmann S, Neugebauer R, Schwartz E, Perret C, Castelli F, Barnett ED, Stauffer WM. Illnessin children after international travel: Analysis from the GeoSentinel SurveillanceNetwork. Pediatrics. In Press.

6Principal Investigator/Program Director(Last, first, middle): Pelican, Katharine M.NAMEPOSITION TITLEKatharine M. Pelican, D.V.M., Ph.D.Assistant Professor of Ecosystem HealthEDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and includepostdoctoral training.)INSTITUTION AND LOCATIONDEGREE(if applicable)YEAR(s)FIELD OF STUDYWesleyan University, Middletown, CT B.A. 1988-1992 Pre-Veterinary MedicineUniversity of Minnesota, Saint Paul, MN D.V.M. 1993-1997 Veterinary MedicineUniversity of Maryland, College Park, MD Ph.D. 1997-2002 Reproductive PhysiologySmithsonian’s National Zoological Park Fellowship 2002-2007 NIH Career Development AwardProfessional Experience2008-date Member, Technical Advisory Committee, Upper Mississippi River Bacteria TMDL Project.Minnesota Pollution Control Agency and Minnesota Department of Health.2008-date Member, Local Foods Task Force, University of Minnesota, St. Paul, MN2009-date Lead, SIGEO Pathogen Program and Pathogen Working Group, Smithsonian TropicalResearch Institute, Panama City, Panama2009-date University of Minnesota Co-Director, USAID RESPOND Program, University of Minnesota,St. Paul, MNPeer-Reviewed Papers and Book Chapters:5. Pukazhenthi, B.S., D. Laroe, A.E. Crosier, L.M. Bush, R. Spindler, K. Pelican, M. Bush, J.G. Howardand D.E. Wildt. 2006. Challenges in cryopreservation of clouded leopard (Neofelis nebulosa)spermatozoa. Therio 66: 1790-1796.6. Pukazhenthi, B.S., K.M. Pelican, and D.E. Wildt. 2007. ‘Appendix A: Genome Resource Banking.’Eds. C. Gascon, J. Collins, R. Moore, D. Church, J. McKay, and J. Mendelson. AmphibianConservation Action Plan. The World Conservation Union (IUCN), Gland Switzerland. Pp. 38-39.7. Pelican, K.M., M.A. Ottinger, D.E. Wildt and J.G. Howard. 2008. Ovarian suppression with theprogestin levonorgestrel but not the GnRH antagonist antide induces a consistent response togonadotropin stimulation in the domestic cat. Dom Animal Endocrinol 34: 160-175.8. Moen, R, C.L. Burdett, K.M. Pelican, and J.M. Rasmussen. 2010. Hematology, serum chemistry, andbody mass of free-ranging and captive Canada lynx in Minnesota. J. Wildlife Dis. 46 (1): 13-22.9.Sepuĺveda M. A., Rosenfeld , C., Jara, R., Pelican, K.M. Prevalence of antibodies to Toxoplasma gondii inferal American minks at the Mauillin River, Chile. J. Vet Parsit. In Revision.10. Pelican, K.M., Abaigar, T. Hormone profiles in the Iberian Lynx: Applications to in-situ and ex-situconservation. In. Iberian Lynx Ex-situ Conservation: An Interdisciplinary Approach. Eds. A. Vargas,Christine Breitenmoser & Urs Breitenmoser. Fundación Biodiversidad & IUCN Cat Specialist Group.In press.11. Stewart, R.A., K.M. Pelican, J.L. Brown, D.E. Wildt, M.A. Ottinger, and J.G. Howard.2010. Oral progestin induces rapid, reversible suppression of ovarian activity in the cat. GenComp Endocrinol 166(2): 409-416.

7BIOGRAPHICAL SKETCHProvide the following information for the Senior/key personnel and other significant contributors in the order listed on Form Page 2.Follow this format for each person. DO NOT EXCEED FOUR PAGES.NAMESrinand SreevatsaneRA COMMONS USER NAME (credential, e.g., agency login)POSITION TITLEAssociate ProfessorEDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, include postdoctoral training andresidency training if applicable.)DEGREEINSTITUTION AND LOCATIONMM/YYFIELD OF STUDY(if applicable)University of Agricultural Sciences, Bangalore DVM 08/86 Veterinary MedicineUniversity of Agricultural Sciences, Bangalore MVSc 08/88 Veterinary MedicineUniversity of Minnesota MPH 04/91Epidemiology (InfectiousDisease)University of Minnesota PhD 05/95 Infectious diseaseBaylor College of MedicinePostdoctoralfellow01/97 TuberculosisPositions and Honors1986-1989 Veterinary Practitioner, Bangalore, India1989-1995 Research Assistant, University of Minnesota, MN1997-2001 Director, Assay Development and Validations, ClinCyte, LLC, San Diego,CA2001-2005 Assistant Professor, The Ohio State University, Wooster, OH2005-Present Associate Professor, University of Minnesota, Saint Paul, MN2007-Present Adjunct Associate Professor, CIDMTR, UMN2008 Adjunct Appointment in Department of Animal Sciences, UMNSelected Peer-reviewed Publications1. Fecteau ME, Ross J, Tennent-Brown BS, Habecker PL, Sreevatsan S, Sweeney RW,Whitlock RH. Mycobacterium avium ssp. paratuberculosis High Shedding in an AdultFemale Alpaca, and its Implications for the Rest of the Herd. J Vet Intern Med.2009 Nov-Dec;23(6):1311-4. PubMed PMID: 19909432.2. Pithua P, Wells SJ, Godden SM, Sreevatsan S, Stabel JR. Experimental validation of anested polymerase chain reaction targeting the genetic element ISMAP02 for detectionof Mycobacterium avium subspecies paratuberculosis in bovine colostrum. J Vet DiagnInvest. 2010 Mar;22(2):253-6. PubMed PMID: 20224087.3. Pithua P, Wells SJ, Sreevatsan S, Godden SM. Lack of evidence for fecal shedding ofMycobacterium avium subsp. paratuberculosis in calves born to fecal culture positivedams. Prev Vet Med. 2010 Feb 1;93(2-3):242-5. Epub 2009 Dec 16. PubMed PMID:200155574. Lamont EA, Sreevatsan S. Paradigm redux-Mycobacterium avium subspeciesparatuberculosis-macrophage interactions show clear variations between bovine andhuman physiological body temperatures. Microb Pathog. 2010 Feb 25. [Epub ahead ofprint] PubMed PMID: 20188816.