PR648 User Manual – English - Hoefer Inc

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Page finderIntroduction...........................................................1Specifications........................................................2Procedure for standard use ofthe Hoefer PR648 .................................................3Setting up the Hoefer PR648 ............................3Applying your samples........................................5Removing your blot............................................5Care and maintenance............................................6Troubleshooting......................................................7Ordering information..............................................8• pi
IntroductionSlot-and dot-blotting techniques have been usedextensively in molecular biology to immobilizenucleic acids and proteins on membranes fordetermining nucleic acid homologies, quantifyingmRNA, studying hormone receptor binding,detecting protein-nucleic acid interactions, andscreening for specific proteins by activity orantibody. The slot-shaped blots produced bythe Hoefer ® PR648 slot blot filtration manifoldare more reliably and accurately quantitatedby scanning densitometry than dot blots. For aseries of dots, the scan path must pass directlythrough the center of all the dots. Slots are notso demand ing in alignment and allow muchwider representative scan paths.The Hoefer PR648 slot blot filtration manifoldis designed and manufactured to provide anefficient seal around each slot insuring a consistentshape and size. As little as 50 µl of sampleapplied to a membrane through the slot willyield a clearly defined band with evenly distributedsample.Each Hoefer PR648 slot blot is comprised ofthree separate blocks. The upper block has48 labeled slots for samples, arranged in a4 × 12 array. The blotting membrane is placedin a recess on the top of the middle (membranesupport) block. The bottom block has a connectorfor the vacuum source.To assemble the Hoefer PR648 slot blot, simplyplace the membrane in the membrane supportblock recess, stack the blocks together, andinsert and tighten the screws. Attach the connectoron the bottom block to a vacuum source andapply your samples in the slots.• p1
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PR648 User Manual – English - Hoefer Inc

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