RNA Cell HTS 96 Kits

Scheme of the InviTrap ® RNA Cell HTS 96 Kit/ CPlease read protocols prior the start of the preparationRemoving the culture medium and cell extractionAdd 250 µl DTT-containing Lysis Solution SMix thoroughly, 20 sec.Place the DNA Binding Plate in a 0.5 ml Collection PlateTransfer lysates in DNA Binding PlateIncubate for 1 min., seal with a foilCentrifuge DNA Binding Plate/ 2ml Collection Plate for 3 min. at4.000 U/min.Discard DNA Binding PlateAdd 230 µl Binding Buffer R to the eluated lysateMix thoroughly by pipetting up and downPlace RNA Binding Plate in a 2.0 ml Collection PlateTransfer the eluates in the RNA Binding PlateIncubate for 1 min., seal with a foilCentrifugate RNA Binding Plate/2ml Collection Plate for 3 mi.n at4.000 U/min.Remove foilAdd 600 µl Wash Buffer R 1, sealingCentrifuge DNA Binding Plate/2ml Collection Plate for 3 min at4.000 U/minremove foil, discard eluatsAdd 400 µl Wash Buffer R 2, sealingCentrifuge DNA Binding Plate/2ml Collection Plate for 3 min at4.000 U/minremove foil, discard eluatsAdd 400 µl Wash Buffer R 2, sealingCentrifuge RNA Binding Plate/2ml Collection Plate at min 4000U/min for 10 min for ethanolremoval, remove foil, discard eluatPlace RNA Binding Plate onto RNase free Microtiter PlateAdd 60-80 µl Elution Buffer R, incubate for 2 min, sealingCentrifuge RNA Binding Plate/ Microtiter Plate for 3 min at 4.000U/minDiscard RNA Binding PlateSeal Microtiter plate, place on ice or store at – 80°C12 InviTrap ® RNA Cell HTS 96 Kit/ C and V 0810