RNA Cell HTS 96 Kits

Product characteristic of the InviTrap ® RNA Cell HTS 96-KitStarting material Yield Timeup to 5 x 10 5 human oranimal cells(cultured cells)max: 15-20 µge.g.10 - 15 µg total RNA from5 x10 5 NIH 3T3 fibroblasts5 µg total RNA from 1 x 10 5 MRC 5 cellsca. 35 min. using a centrifugeca. 40 min. using a vacuum manifoldca. 30 min. using centrifuge incombination with a vacuum manifoldThe InviTrap ® RNA Cell HTS 96 Kit/ C provide a fast and efficient way for the isolation of highquality total RNA from 96 – 192 RNA samples from max. 5 x 10 5 human or animal cells (cellculture), inter alia for field of research like agent-screenings, therapy monitoring, basic research,expressions profiling, and molecular diagnostic.The InviTrap ® RNA Cell HTS 96 Kit/ C is designed for use on a centrifuge, the InviTrap ® RNACell HTS 96 Kit/ V for use in combination with the Invisorb ® 96 Vacuum Manifold.In less than 30 min. 192 samples can be processed gaining highly purified RNA.Special buffer conditions guarantee an efficient lysis of the starting material and an inactivation ofendogenous RNases. Genomic DNA-contaminations are almost completely separated from thetotal RNA by binding to a newly developed carrier substance.No enzymatic step, like DNase-digestion, to remove contaminating genomic DNA is necessary.Subsequent RNA is bound onto the membrane of the RNA Binding-Plate. Contaminants areremoved by following wash steps and high purified RNA can be eluted in small volume of RNasefree water.The extracted RNA contains enriched mRNA. The particle size distribution of purified RNA issimilar to those, gained using a CsCl – gradient.The procedures require minimal interaction by the user, allowing safe handling of potentiallyinfectious samples. The procedures are optimized to avoid sample-to-sample cross-contamination.Due to the high purity, the isolated total RNA is ready to use for a broad panel of downstreamapplications (see below) or can be stored at –80°C for subsequent use.Downstream Application○○○○○○RT-PCRDDRT-PCRReal-time PCR (quantitative RT-PCR, like TaqMan ® und LightCycler ® technologies)cDNA synthesesMirco array analysisNorthern Blot analysisNo toxic or hazardous chemicals like phenol/chloroform or ß-Mercaptoethanol are used. Traditionaltime-killing procedures can be replaced using the InviTrap ® RNA Cell HTS 96 Kit.For automated high throughput needs Invitek offers the InviTrap ® RNA Cell HTS 96 Kit/ R (seeorder information).Invitek also offers a column based system for the puification of RNA from cells in a single tubeformat. For small amounts of cells the InviTrap ® Spin Cell RNA Mini Kit and for larger samplequantity the InviTrap ® Spin Cell RNA Midi Kit, using the same procedure.If you are interested in using the kit on a laboratory workstation, please do not hesitate to contactour technical support (++49 (0)30-9489-2910/ 2901/ 2907).6 InviTrap ® RNA Cell HTS 96 Kit/ C and V 0810