RNA Cell HTS 96 Kits

11. Repeat this washing step by adding again 400 µl Wash Buffer R2 to each well of the RNA-Binding Plate. Switch on vacuum source and apply vacuum until the transfer of the wash bufferis complete (about 2 min). Switch off vacuum and ventilate the vacuum manifold.12. To remove adherent fluid, lift the RNA-Binding Plate from the InviTrap ® Top Plate and strike thebottom side of the RNA-Binding Plate on a stack of paper towels. Repeat several times until nofurther liquid is released onto the paper. Remove the manifold top plate from the base anddiscard the flow-through from the waste tray.13. Place the waste tray inside the vacuum manifold and close the manifold with the Top Plate. Placethe RNA-Binding Plate onto the rubber joint of the Invisorb ® 96 Vacuum Manifold top plate. Switchon vacuum source and apply vacuum for at least 20 min for complete removal of all ethanol.Switch off vacuum and ventilate the vacuum manifold. Take the RNA-Binding Plate out of themanifold and place it on a clean paper towel. Remove the manifold top plate and the waste tray.14. Place the spacer for Microtiter Plates (large spacer) followed by the Microtiter Plate inside thebase of the Invisorb ® 96 Vacuum Manifold and close the manifold with the top plate.15. Place the RNA-Binding Plate onto the rubber joint of the Invisorb ® 96 Vacuum Manifold Top Plate.For the elution of total RNA add 60-80 µl Elution Buffer R (RNase-free) directly onto themembrane in each well of the RNA-Binding Plate and incubate for 2 min.16. Switch on vacuum source and apply vacuum for at least 3 min until the transfer until the transferinto the wells of the Microtiter Plate is complete. To remove adherent drops from the outlets of theRNA-Binding Plate strike several times with the flat hand on the top of the RNA-Binding Plate.17. Switch off vacuum and ventilate the vacuum manifold. Take the RNA-Binding Plate verycarefully out of the manifold in order to avoid cross-contaminations with adherent fluid. Discardthe RNA-Binding Plate. Remove the manifold top plate from the base and take the MicrotiterPlate with the eluted total RNA out of the manifold. Cover the Microtiter Plate for storage (-80°C).Note: Invisorb ® 96 Vacuum Manifold – Please follow the instruction on page 20.Note: The Invisorb 96 Vacuum Manifold contains acrylglass. To avoid any Problem with the materialNote:1. Avoid a long incubation with ethanol or isopropanol containing solutions2. Clean up the manifold after using with water and dry it.3. Always use the waste tray.16 InviTrap ® RNA Cell HTS 96 Kit/ C and V 0810