Standard Operating Procedure for Escherichia coli - Division of ...

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$Lagoon System Math - Division of Water Quality$

$Exam Math Review Collection Grades I & II Small Lagoon System ...$

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Version 38-27-09SWfluorescent in the “Quanti-Tray/2000 Positive LargeWells (0-49)” space and the number of small wellsare both yellow and fluorescent in the “Quanti-Tray/2000 Positive Small Wells (0-48)” space.Click on the “Calculate” button. The results arerecorded in MPN/100mL. Record these results onthe bench sheets. The MPN Generator also givesyou the 95% confidence limits.g. Click on the “Log” button to store these results inthe automatically created spreadsheet.b. To calculate for duplicate precision follow the steps outlined in the“Standard Methods for the Examination of Water and Wastewater” (20 thEdition). See attached Appendix.c. The blank samples should have a value of
Version 38-27-09SWFigure 1. Overview of E. coli and Total coliform Quantification.Collect water samples in 100mL sterile containers.Store samples on wet ice up to 8 hours.Pour 1 packet of Colilert reagent into each sample. Shake.Pour solution into Quanti-Trays and seal the trays.For TCIncubate for 24 – 28 hours at 35C.For E. coliCount # large &small wells.Count # large & smallwells that are bothyellow and fluorescent.Enter # of wells inMPN Generator.Enter # of wells inMPN Generator.Enter counts(MPN/100ml) onbench sheet.Enter counts(MPN/100ml) onbench sheet.
Page 5 and 6: Version 38-27-09SWiv. If the yellow
Page 7: Version 38-27-09SWc. Enter your sam
Page 11: Version 38-27-09SWStandard Operatin

Standard Operating Procedure for Escherichia coli - Division of ...

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