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4LIONE et al: SURVIVAL OF Streptococcus agalactiae WITHIN HUVECsFigure 2. Acidification and maturation of GBS-containing vacuoles inside endothelial cells (HUVECs). Photomicrographs of acidic compartments of endothelialcells submitted to fluorescence orange acridine labelling showing the acidification of GBS-containing vacuoles inside HUVECs (A-C) after 1 h incubation withbacteria. Lysosomal-endocitic pathway compartments involved in the internalization of GBS, double-marked by anti LAMP-1 (D-F) and anti RAB-7 (G-I) wereperformed after 1 h incubation with DAPI-labeled GBS grown at 40˚C. Arrows indicate GBS in whole and close-up images. Scale bar is 20 μm.

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