1 GENERAL BIOLOGY LAB 1 (BSC1010L) Lab #10: Biotechnology ...

4. Record your results Table 2.Table 2:ConditionLB -Results(growth/no growth)Type of GrowthObserved(lawn/colonial)Did your results agree with yourpredictions in Table 1?(if not, provide a possibleexplanation)LB/Amp -LB +LB/Amp +5. Calculate transformation efficiency.Transformation efficiency is a measure of how successful host cells are in uptaking theplasmid, where uptake is expressed as the number of transformed colonies per µg ofplasmid DNA.Note: For this exercise, you will need to count the number of colonies present on yourLB/Amp+ plate. Follow steps a through e to calculate the transformation efficiency ofyour E.coli strain.a. The total amount (µg) of plasmid DNA used can be calculated with the followingformula.µg DNA = concentration (µg/µL) of DNA x volume of DNA (µL)b. Calculate the total volume of cell suspension prepared in the + DNA tube.Total volume (µL) = amount (µL) of CaCl 2 + amount (µL) of plasmid + amount (µL) of LB10