1 GENERAL BIOLOGY LAB 1 (BSC1010L) Lab #10: Biotechnology ...

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f. Centrifugationg. Plasmidh. Aseptic Techniques2. What factors could have affected transformation efficiency?3. What transformation efficiency would you expect for all the other treatments?4. How would you improve or extend the experiment above?5. In nature, DNA uptake by different organisms can impart advantages as well asdisadvantages to the host organism.a. When would genetic transformation be advantageous to a host organism?b. When would genetic transformation be maladaptive to a host organism? Whatconsequences would there be for the host cells?12
5. Can you think of a case where the uptake of foreign DNA would be advantageous forthe organism, but disruptive for the surrounding ecosystem?______________________________________________________________________________LOOK AHEAD:Before coming to lab next week, make sure to read the Population Genetics task sheet as well asChapter 18 (pgs 191-197) in your lab manual.13
Page 3 and 4: Table 1:LB -Condition Expected resu
Page 5 and 6: Figure 17. Return the + DNA tube to
Page 7 and 8: 17. Using a sterile transfer pipett
Page 9 and 10: A. B.Figure 6. Plates illustrating
Page 11: c. Since only a portion of the + DN

1 GENERAL BIOLOGY LAB 1 (BSC1010L) Lab #10: Biotechnology ...

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