Factors Affecting Enzyme Activity: Lactose Intolerance
Factors Affecting Enzyme Activity: Lactose Intolerance
Factors Affecting Enzyme Activity: Lactose Intolerance
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<strong>Factors</strong> <strong>Affecting</strong> <strong>Enzyme</strong> <strong>Activity</strong>Start with stock ONPG solutionDilution factorDilution No Dilution1.0 mL1.0 mLStockONPGsolution1.0 mLStockONPGsolutionStockONPGsolution2.0 mLH 2O1.0 mLH 2O0.5 mLH 2O11 + 2 = 0. 11 +1 = 0.50 11 + 0.5 = 0.670.5 mL0.5 mL0.5 mL0.5 mL1.0 mLCuvette#1Cuvette#2Cuvette#Cuvette#4Cuvette#5StockONPGsolution1.5 mLCuvette#610