13.07.2015 Views

Factors Affecting Enzyme Activity: Lactose Intolerance

Factors Affecting Enzyme Activity: Lactose Intolerance

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C. Effect of Substrate Concentration<strong>Factors</strong> <strong>Affecting</strong> <strong>Enzyme</strong> <strong>Activity</strong>Cuvette #Relative SubstrateConcentrationAbsorbanceBlank 0 01 0.332 0.503 0.674 15 26 3<strong>Enzyme</strong> <strong>Activity</strong> vs. Substrate Concentration2.01.5Absorbance1.00.50.00.00 0.50 1.00 1.50 2.00 2.50 3.00Relative Substrate ConcentrationQ.5 What happens to the rate of an enzyme-catalyzed reaction as substrate concentration israised beyond the saturation point?15

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