Biomek® Cell Workstation - Beckman Coulter

Process toSolutionSoftware• SAMI ® Process Managementsoftware to organize paralleland interleaved processes.Includes a calendar view tosimplify laboratory resourcemanagement.Biomek ® Cell Workstation enables fully automated cell seeding,growth rate monitoring and quality control, cell harvesting andplating into microtiter plates.Different cell lines can be cultured in parallel without user interventionover days, weeks or even months. Advanced softwarecontrol allows true 24/7 operation.Monolayer Cell Culture Processcell culture flask,confluentmonolayer of cell10 ml PBS toremove cell culturemedium/serum1 ml trypsin/EDTA oncell monolayer todetach cells• SAMI ® Process Definition Editorenables users to graphically definecomplex processes consistingof multiple tasks.• Intuitive graphic editor to defineand schedule individual tasks indetail. Integrated data trackingprovides on-the-fly feasibilitychecking of these tasks.incubation (3-5 days)medium10mlbuffer10 ml buffer1 ml trypsinEDTA9 ml cell culturemedium (37°C)10 ml cellsuspensionIncubation(3-10 min, 37°C)• Pipetting actions can be optimizedfor any cell type, liquidclass and and labware type.max. 20 new cellculture flasks5 (5x10 cells)3 microplates,96 wells,30.000 cells/wellBiomek ® Cell Workstation is based on the Biomek ® liquid handlerand includes new hard- and software components to automatecell culture applications.The system can be used with both adherent and suspensioncell cultures and can be easily optimized for different growthcharacteristics. Advanced planning and scheduling tools allowmultiple tasks such as cell line expansion and media exchangeto occur simultaneously.15 ml cell culturemedium per flask0,5 ml cell suspensionin new flasks150 l mediumper well33 l cell suspensionper well0,5 ml cellsuspension9,5 ml cellsuspension (ca.10 7 cells)cell counting,viability test• Unique integrated data management,i.e. tracking, logging andreporting. Any information aboutany sample, well, or plate isregistered in a database andavailable for later use. Labwarereports provide information aboutevery single plate in the systemat any point of time.• Data management systemincludes SQL viewer for easyLIMS connectivity.