Name: José A.B.M. FEIJÓ Birthday: 24-02-1962 Nacionality ...

Name: José A.B.M. FEIJÓBirthday: 24-02-1962Nacionality: PORTUGALDivorced, father of Pedro (21) and Joana (16)Affiliation: Universidade de Lisboa, Fac. Ciências (www.fc.ul.pt)(until September 2013)and Instituto Gulbenkian de Ciência (www.igc.pt)PortugalEmail: jfeijo@fc.ul.ptURL http://www.igc.gulbenkian.pt/code/research.php?lang=en&unit_id=38Academics:Full Professor Aggregation, Univ. Lisboa, 2001Fullbright Senior Fellow, Univ. Massachussets, 1996PhD. Cell Biology (w/ honours), Univ. Lisboa, 1995Master, Plant Biotechnology (Excellent) 1989Licenciatura (BSc-5 years), Biology- scientific branch, (18/20), 1986Professional Track:2006-2013: Professor Catedrático (Full Professor) Faculdade de Ciências daUniversidade de Lisboa2005-…: Director- Cell Imaging Unit Instituto Gulbenkian de Ciência1999-…: Resident Researcher- Instituto Gulbenkian de Ciência2010-2012: Member of the scientific research council for the natural sciences andenvironment, FCT (Fundação para a Ciencia e Tecnologia; government’s sciencefunding agency in Portugal)2006-2011: Curator of the exhibition “Darwin’s Evolution” developed for theGulbenkian Foundation, Portugal, on display from Feb-May 2009(www.gulbenkian.pt/darwin/ ; http://www.jffprv.eu/webo/links/index0080cibioexpdwn.html ). The exhibition toured to the “Museo Nacionale deCiencias Naturales”, Madrid, Jul09-Jan10, the science park in Granada, Feb-Aug2010, and University of Porto, Feb-July 2011, reaching a total of ca. halfmillion visitors and ca. 3000 school groups.2003-2007: President (head/chair) of Department (Plant Biology Dept., Fac. CiênciasUniv. Lisboa)2003-2011: Founding member and first coordinator (2003-2006) of theDevelopment Biology Research Center (cbd.igc.gulbenkian.pt) at the IGC;research centers in the Portuguese system are autonomous research units thatreceive independent, no-strings funding, according with international peerreviewedevaluation. The DevBiol. Center as evaluated 3 consecutive times as

EXCELLENT, ranking on the top 5% of the country’s research centers for allareas (including social sciences)2000-2006: Associate Professor, Univ. Lisboa1995-2000: Assistant Professor, Univ. Lisboa1990-1995: PhD student and TA, Univ. Lisboa(1988/89: Compulsory military service)1987-1995: Teaching Assistant, Plant Biology Dept., Univ. Lisboa1985-1987: High School teacher (Biology, K-7 to K-12)Academic TeachingCitology (Labs)- 1st. yr Biology (1986/87, 90-91, 93/94, 96/97, 98/99)Cell Biology (labs)– 1st yr Biology (1999/2000; 2000/2001)Cell Biology (Labs)- 2nd yr Biochemistry (1987/88, 88/89, 90/91, 91/92)Advanced Cytology (Labs)- 3rd yr Biology, (1987/88), (1993/94)Plant Cell Biology- 3rd yr Applied Plant Biology (1988/89, 90/91, 91/92)Plant Cell Culture- 3rd yr Applied Plant Biology (1989/1990)Advanced Cell Biology (Regency/theoreticals)- 4th yr Biochem. (2001/2002)Plant Devolpment (Author of both theoretical and practical program;Regency/theoreticals/seminars)- 94-2013 (w/ interruption on the years itall1- Em 1994 foi incumbido da regência da cadeira de Diferenciação e Morfogénesedos Vegetais. Esta cadeira foi criada pela primeira vez nessa data, no âmbito daremodelação das licenciaturas em Biologia, pelo que se tratou de pensar e montaruma cadeira sem qualquer ocorrência prévia. Foi responsável pela elaboração dosprogramas teórico e prático. A cadeira foi iniciada em Outubro de 1994,funcionando como obrigatória para os 3º ano da licenciatura de Biologia VegetalAplicada e como opção para as restantes, tendo sido frequentada por alunos dos3º e 4º anos do ramo Científico, Biologia Microbiana e Genética e BiologiaAplicada aos Recursos Animais. Desde então (1994/95, 95/96, 96/97, 97/98,98/99; 2003/4) foi responsável pela regência, aulas teóricas e práticas (nos anos1999/2000 e 2000/2001 foi responsável somente pelas práticas). Ainda no âmbitodesta cadeira tem sido responsável pela organização de seminários interdisciplinarespúblicos realizados pelos alunos e foi mentor e editor dum fascículotemático sobre Desenvolvimento em Plantas pela revista Ciência (editado pelaAssociação de Estudantes da Faculdade de Ciências da Universidade de Lisboa).2- Esta cadeira deu origem à cadeira de Biologia do Desenvolvimento em Plantasna re-modelação que teve lugar em 2004/2005, de que foi e continua a ser regente(2008/09, 2009/10, 2010/11).

- Along 8 years taught theoretical and practical course on the subjects of CellBiology, Advanced Cell Biology, Plant Biotechnology and Plant Tissue Culture- In 1995 created the undergrad optional Course “Plant Development” (6 ECTS:3T+3P). This course has meanwhile become mandatory curricula in all Biologybranches (except environment specialities) and runs yearly with ca.120 students.- In 2003 created the undergard course “Introduction to Systems Biology”- Member of the scientific board of the degree on “Health Sciences”; co-creatorand coordinator the Master “BioInformatics and Computational Biology” (bothto end in December 2012).Supervision and Grants(1996-…)- 20+ Master thesis (or equivalent 1 year full-time research projects)(unless otherwisespecified all in Biology/Biochem degrees)Rui M.Malhó(87-89), Cristina M.Marques(87/88), Adelaide Simões(89/90),Rui G. Martinho(92/93), Ana Lourenço and M.Cristina Gonçalves(99/00)(Applied Physics), Sofia Cordeiro (98/99), Ricardo Almeida (99/00),Ana Margarida M.Prado (99/00), Sílvia C.S.P. Costa (99/00), Ana MariaVieira (99/00), Nuno Geraldo (00/01), Catarina Silva (00/01), Joana Vital(00/01), José Antão(01/02), Daniel Vieira(02/03), Liliana Freitas (02/03),Ricardo Henriques (Physics Eng., 04/05), Renato Colaço (05/06), BárbaraSantos (05/06), Pedro Dias (Physics Eng., 05/06),- 15+ PhD successful projects (and 20+ co-orientations)- 12+ PostDoc supervisions- 12 national grants; 3 european grants; participation in one NSF grant; 2 grants withforestry Companies(fellowships and grants generating a gross total of ca. 3.2 M€ (~4.2 M USD) from 1997-2010; in the same period managed institutional equipment grants in a gross total of ca.2.5 million Euro, and a gross total budget of ca. 1.5 million Euro for the exhibition“Darwin’s Evolution”)Funding (2013- ):0. Fellowships- In the portuguese system, fellowships can be obtained independentlyof grant proposals. Over the past 10 years I successively sponsored 10 PhD/4yrfellowships (~0.5M€) and 12 PostDoc/3yr fellowships (~0.8M€)1. “Whole genome approach to ion dynamics regulation of pollen tube growthand morphogenesis” 2002-2005 (225.000 €; included leverage granting for the firstAffymetrix station in Iberia)2. “A genetic and biophysics approach to cell-cell communication in sexual plantreproduction” 2003-2006 FCT POCTI/BCI/46453/2002 (92.040 €)

3. “Oscillatory behaviour of pollen tubes: bfundamental principles of biophysicaland biochemical mechanisms underpining the regulation of cellular growth andmorphogenesis” POCI/BIA-BCM/60046/2004 (120.000 €)4. “A comparative analysis of the Arabidopsis Sperm Cell Transcriptome todecipher the role of the male gametes in double fertilization” POCI/BIA-BCM/61270/2004 (120.000 €)5. “Electrophysiological characterization of membrane transporters fromArabidopsis thaliana pollen tube” PTDC/QUI/64339/2006 (65.000 €)6. “A systems approach to apical cell growth” PTDC/BIA-BCM/108044/2008(200.000 €)(CURRENT FUNDING; includes research networks)7. PlantOrigins; EU-PITN-GA-2009-238640 (coordinator: Prof. Liam Dolan,U.Oxford; other partners: Nat.HistoryMuseum-London; Univ. Wurzburg; CzechAcad Sci-Prague)(IGC total- 220.000€)8. Harnessing plant reproduction for crop improvement; EU COST Action FA0903;(total of 35 european partners)(IGC total- 50.000€)9. RCN on Integrative Pollen Biology; NSF 0955910 (coordinator: Prof. AliceCheung, Univ. Massachusetts, Amherst, US)(no direct costs, cover trips andparticipation in courses)10. The role and function of pollen tube glutamate receptor-like (GLR) channels;Jan2013-Dec2014; 200K€ (261K USD)(FCT- BEX-BCM/0376/2012)11. Cell size measurement during pollen tube growth and the evolutionary success ofthe angiosperms; Jan2013-Dec2014; 180K€ (235K USD) (FCT- PTDC/BIA-PLA/4018/2012)12. (Coordinator, multi-PI equipment grant) Imaging structure and dynamics in livingorganisms; Jan2013-Dec2015; 0.5M€ (652K USD); involves the acquisition andfabrication of two systems of super-resolution (STORM) and FluorescenceCorrelation Spectroscopy/Microscopy (FCT- RECI/BEX-BCM/0083/2012)International Course Organization- 1997-2000: International Workshop on “Plant Development: the cellular andmolecular basis”; offered yearly to the Gulbenkian and other PhD programs;organizer- 2001-2004: EMBO Practical Course on “Plant Development: the cellular andmolecular basis” 3 week practical course, offered yearly to 20 internationalstudents; organizer- 2003/2005/2007/2009: EMBO Practical Course on “Live Cell Imaging”; coorganizer- 2008/2010/2012: EMBO Practical Course on “3-D Developmental Imaging”;co-organizer- 2009/2011/2013- EMBO workshop on “The Biophysical basis ofDevelopment”; member of the Scientific Committee.

Bibliometrics (WoK, March 2013)

Peer-Reviewed Original Papers(1) Becker JD, Takeda S, Borges FS, Dolan L and JA Feijó (2012) TranscriptionalProfiling of Arabidopsis Root Hairs and Pollen Defines an Apical GrowthSignature. Molecular Plant (submitted)(2) Vieira AM, Feijó JA- (to be submitted January 2013 to Molecular PlantspecialIssue on Plant Reproduction)(3) Boavida, LC, Becker JD and José A. Feijó (2012) The Arabidopsis TOCA-E-FOGE is required for pollen-stigma adhesion by affecting cell wall and cuticlebiosynthesis. Plant Physiology (under review for re-submission)(4) Calarco JP*, Borges FS*, Donoghue MTA, Van Ex F, Jullien PE, Lopes T,Gardner R, Tang C, Berger F, Feijó JA, Becker JD, Martienssen RA (2012)Reprogramming of DNA methylation in pollen guides epigenetic inheritancevia small RNA. Cell, 151: 194–205(5) Tavares B, PN Dias, TF Moura, JA Feijó and A Bicho (2011) CalciumRegulated Anion Channels from Pollen of Lilium longiflorum. New Phytologist192: 45–60(6) MICHARD, E., LIMA, P.T., PORTES, M.T., BORGES, F.S., SILVA, A.C.,CARVALHO, J.E., LIU, L.H., GILLIHAM, M, OBERMEYER, G. andFEIJÓ, J.A. (2011) Glutamate Receptors and D-serine modulate the Ca2+signature and growth of pollen tubes. Science 332:434-437 Cover issue.F1000-FFa-14(7) Boavida, L, Borges, FS, Becker, JD, Feijó, JA (2011) A spatial and temporalprofile of gene expression during pollen-pistil interactions in Arabidopsis.Plant Physiology, 155:2066–2080(8) Zhao Y, Yan A, Feijo JA, Furutani M, Takenawa T, Hwang I, Fu Y, Yang Z(2010) Phosphoinositides regulate clathrin-dependent endocytosis by at the tipof pollen tubes in Arabidopsis and Tobacco. Plant Cell, 22:4031-4044(9) Cheung, AY, Boavida L, Aggarwal M, Wu HM, Feijo JA (2010) The PollenTube Journey and Guidance to the Female Gametophyte. J Exp Bot 61:1907-1915; Cover Issue(10) Ramos, AC, Lima, PT, Dias, PN, Kasuya, MCM, Feijó, JA (2009) A pHsignaling mechanism involved in the spatial distribution of calcium and anionfluxes in ectomycorrhizal roots. The New Phytologist 181 (2): 448-462(11) Ramos AC, Martins MA, Okorokova-Façanha AL, Olivares FL, OkorokovLA, Sepúlveda N, Feijó JA, Façanha AR (2009) Arbuscular mycorrhizal fungiinduce differential activation of the plasma membrane and vacuolar H + -pumpsin maize root sytem. Mycorrhyza 19:69-80

(12) Slotkin RK, Vaughn M, Tanurdžić M, Borges FS, Becker JD, Feijó JA,Martienssen RA (2009) Reactivation of transposable elements in thevegetative nucleus of pollen. Cell 136:461-472. F1000- FFa- 13(13) Certal, AC, Almeida, RB, Wong, S, Moreno, N, Carneiro, J, Rodriguéz-Léon, J, Wu, HM, Cheung, AY, Feijó, JA (2008) Exclusion of a protonATPase from the apical membrane is associated with cell polarity and tipgrowth in Nicotiana tabacum pollen tubes. The Plant Cell 20(3): 614-634.F1000- FFa-6(14) Ramos, AC, Façanha, AR, Feijó JA (2008) A H + Ion Flux Signature for theHyphal Asymbiotic Development of the Mycorrhizal Fungus Gigasporamargarita. The New Phytologist 178(1):177-188 Cover Issue(15) Borges F, Gomes G, Gardner R, Moreno R, McCormick S, Feijó JA, BeckerJD (2008) Comparative Transcriptomics of Arabidopsis Sperm Cells. PlantPhysiology 148:1168-1181(16) Michard E, Dias P, Feijó JA (2008) Tobacco pollen tubes as cellular modelsfor ion dynamics: improved spatial and temporal resolution of extracellularflux and free cytosolic concentration of calcium and protons using pHluorinand YC3.1 CaMeleon. Sexual Plant Reproduction. 3:169-181(17) Prado, AM, Colaço, R, Moreno, N, Silva, AM, Feijó JA (2008) Targeting toovules is dependent on nitric oxide signalling. Molecular Plant. 1:703-714(18) Cheung, AY, Duan, QH, Costa, SC, De Graaf, B, Di Stilio, VS, Feijó, JA,Wu, HM (2008) The Pollen Tube Cytoskeleton: Live Cell Studies. MolecularPlant 1:686-702(19) Ramos, AC, Façanha, AR, Lima, PT, JA Feijó (2008) pH signature for theresponses of arbuscular mycorrhizal fungi to external stimuli. Plant Signallingand Behaviour 3:850-852(20) Ribeiro MJ, Silva Pereira C, Soares N, Viera AM, Feijó JA and Jackson P(2006). The contribution of extensin network formation to rapid, hydrogenperoxide-mediated increases in grapevine callus wall resistance to fungal lyticenzymes J.Exp.Bot. 57:2025-2035(21) Pina C, Pinto F, Feijó JA, Becker JD (2005) Gene family analysis of theArabidopsis pollen transcriptome reveals novel biological implications for cellgrowth and division control and gene expression regulation. Plant Physiology138: 744–756 (highlighted as an “High Impact” paper; Grennan AK (2007) AnAnalysis of the Arabidopsis Pollen Transcriptome. Plant Physiology 145:3-4)(22) Cox G, Moreno N, Feijó JA (2005) Second harmonic imaging of plantpolysaccharides. Journal of Biomedical Optics, 10(2):024013/1-6

(23) Raya A, Kawakami Y, Rodriguez-Esteban C, Ibañez M, Rasskin-Gutman D,Rodriguez-Leon J, Büscher D, Feijo JA, Izpisua-Belmonte JC (2004) Notchactivity acts as a sensor for extracellular calcium during vertebrate left-rightdetermination. Nature 427:121-128. F1000- FFa- 12(24) Prado, AM, Porterfield, DM, Feijó, JA (2004) Nitric Oxide is involved inpollen tube growth regulation and re-orientation. Development 131(11):2707-2714.Highlighted in “In this issue” (Development, 131:27 ), “News& Views In Brief” (Nature, 429:620), New Scientist, BBCWildlife, and over 50 media titles(25) Moreno, N, Feijó, JA, Cox, G (2004) Implementation and evaluation of adetector for forward propagated second harmonic signals. Micron 35:721-724(26) Cox, G & Feijó, JA (2004) Second harmonic imaging of plantpolysaccharides. SPIE 5323: 335-342(27) Becker JD, Boavida LC, Carneiro J, Haury M, Feijó JA (2003)Transcriptional profiling of Arabidopsis tissues reveals the uniquecharacteristics of the pollen transcriptome. Plant Physiology 133:713–725(28) Zonia, L., Cordeiro, S., Tupy, J., Feijó, J.A. (2002) Oscillatory ChlorideEfflux At The Pollen Tube Apical Tip Has A Role In Growth AndOsmoregulation And Is Linked To Inositol Polyphosphate Signaling Pathways.Plant Cell 14(9):2233-2249. F1000- FFa- 7(29) Certal, A.C., L. R. Almeida, R. Boskovic, M.M. Oliveira, J.A. Feijó (2002)Structural and Molecular Analysis of Self-incompatibility in almond (Prunusdulcis) Sexual Plant Reproduction 15:13-20(30) FEIJÓ, J.A., G. COX (2001) Visualization of meiotic events in intact freshanthers by means of two-photon microscopy. Micron 32(7):679-684. Coverissue(31) H. TRINDADE, L.C. BOAVIDA, N. BORRALHO, J.A. FEIJÓ (2001) Successfulfertilisation and seed setting from pollination on immature non-dehiscedflowers of Eucalyptus globulus. Annals of Botany 87(4):469-475(32) Boavida, L., Silva, JP, FEIJÓ, J.A (2001) Sexual Reproduction on cork-oak(Quercus suber L.): II- Crossing intra and interspecific barriers Sexual PlantReproduction 14(3):143-152(33) Vissenberg K, FEIJÓ JA, Weisenseel MH, Verbelen JP (2001) Polar fluxesduring the induction of elongation in Nicotiana tabacum cells Journal ofExperimental Botany 52:2161-2167

(34) Antoine, AF, Faure, JE, Dumas, C, FEIJÓ, JA (2001) Differentialcontributions of free cytosolic and extracellular fluxes of calcium to gametefusion and egg activation in flowering plants. Nature-CellBiology 3:1120-1124(35) Cox G, Dibbayawan T, Feijo JA (2001) Multiphoton microscopy of celldivision in plant cells. SPIE 4262:146-154(36) ANTOINE AF, J.-E. FAURE, S. CORDEIRO, C. DUMAS, M. ROUGIER, J.A. FEIJÓ(2000) A calcium influx is triggered and propagates as a wavefront in thezygote after in vitro fertilization of flowering plants. Proc. Natl. Acad. Sci.USA, 97(19):10643-10648(37) FEIJÓ, J.A., J.SAÍNHAS, G. R. HACKETT, J. G. KUNKEL, P.K. HEPLER (1999)Growing pollen tubes have a constitutive alkaline band on the clear cap and agrowth-dependent acidic tip. Journal of Cell Biology 144(3): 483-496(38) BOAVIDA, L., LC, MC VARELA, JA FEIJÓ (1999) Sexual Reproduction oncork-oak (Quercus suber L.): I- The progamic phase. Sexual PlantReproduction 11(6):347-353(39) CERTAL, AC, AM SANCHEZ, H KOKKO, W BROTHAERTS, MM OLIVEIRA, JAFEIJÓ (1999) S-RNAses in apple are expressed in the pistil along the pollentube growth path. Sexual Plant Reproduction 12(2):94-98(40) CÁRDENAS, L, FEIJÓ, J.A., KUNKEL, J., SÁNCHEZ, F., HOLDAWAY-CLARKE,T., HEPLER P.K., QUINTO, C. (1999) Rhizobium Nod Factors Induce Increasesin Intracellular Free Calcium and Extracellular Calcium Influxes in Bean RootHairs. Plant Journal 19:347-352(41) HOLDAWAY-CLARKE T.L., FEIJÓ J.A., HACKETT G.A., KUNKEL J.G., HEPLERP.K. (1997) Pollen tube growth and the intracellular-cytosolic calcium gradientoscillate in phase while extracellular calcium influx is delayed. The Plant Cell.9:1999-2010.(42) MALHÓ, R., J.A. FEIJÓ, M.S. PAIS (1992) "Effect of electrical fields andexternal ionic currents on pollen tube orientation". Sexual Plant Reproduction,5:57-63(43) FEIJÓ, J.A., R. MALHÓ, M.S. PAIS (1992) A cytochemical study on therole of ATPases during pollen germination in Agapanthus umbellatus L´Her.Sexual Plant Reproduction. 5:138-145(44) FEIJÓ, J.A. e M.S.S. PAIS (1989) "Cytomixis in meiosis during themicrosporogenesis of Ophrys lutea: an ultrastructural study". Caryologia 42:37-48(45) FEIJÓ, J.A. e M.S.S. PAIS (1988) "Ultrastructural modifications of plastidsand starch metabolism during the microsporogenesis of Ophrys lutea Cav.(Orchidaceae)". Annals of Botany, 61: 215-219

(46) PAIS, M.S.S., e J.A. FEIJÓ (1987) "Microbody proliferation during themicrosporogenesis of Ophrys lutea". Protoplasma, 138: 149-155Invited Reviews(47) Feijo et al- Cold Spring Harbor Symposia in Quantitavive Biology (to besubmitted, Dec 2012)(48) Maitê- J.Exp. Bot. (2012- submitted to JXB)(49) Colaço R, Moreno, N, Feijó JA (2012) On the fast lane: mitochondriastructure, dynamics and function in growing pollen tubes. J. Microscopy247:106–118(50) Tavares B, Domingos P, Dias PN, Feijó JA and Bicho A (2011) TheEssential Role of Anionic Transport in Plant Cells: The Pollen Tube as a casestudy. J.Exp.Bot. 62: 2273-2298(51) McCue AD, Cresti M, Feijó JA and Slotkin RK (2011) Cytoplasmicconnection of sperm cells to the pollen vegetative cell nucleus: potential rolesof the male germ unit revisited. J. Exp.Bot. 62, 1621–1631,(52) Konrad K, Wudick MM, Feijó JA (2011) Calcium Regulation Of TipGrowth: New Genes For Old Mechanisms. Current Oppinion in Plant Biology14:721–730(53) Feijó JA (2010) The mathematics of sexual attraction. Journal of Biology.9:18(54) Michard, E, Alves, R.F., Feijó, JA (2009) The role of ion fluxes in polarizedcell growth: the pollen tube as an experimental paradigm Int.J.Dev.Biol. 53:1609-1622, doi: 10.1387/ijdb.072296em(55) Lima PT, Faria VG, Patraquim P, Ramos AC, Feijó JA, Sucena E (2009)Plant-microbe symbioses: new insights into common roots. BioEssays31(11):1233-1244(56) Becker, JD, Feijó, JA (2007) How many genes are needed to make a pollengrain? Lessons from transcriptomics. Annals of Botany, 100:1117-1123 CoverIssue(57) Boavida, L, Becker, JD, Feijó, JA (2005) The making of gametes in higherplants. Int.J.Dev.Biol. 49:595-614(58) Boavida, L, Becker, JD, Vieira, AM, Feijó, JA (2005) Gametophyteinteraction and Sexual Reproduction: how plants make a zygote. Int.J.Dev.Biol.49:615-632(59) Feijó, JA, Costa, S, Prado, AM, Becker, JD, Certal, AC (2004) Signaling bytips. Curr.Op.Plant Biol. 7:589-598

(60) Feijó, JA, Moreno, N (2004) Two-photon microscopy applications to plantcells. Protoplasma 223(1):1-32(61) Zonia, L., S.Cordeiro and J.A. FEIJÓ (2001) Ion dynamics and the control ofhydrodynamics in the regulation of pollen tube growth. Sexual PlantReproduction 14(1-2):111-116(62) Antoine AF, Dumas C, Faure J-E, FEIJÓ JA, Rougier M (2001) Eggactivation in flowering plants. Sexual Plant Reproduction 14(1-2):21-26(63) FEIJÓ, J.A., J. SAINHAS, T. HOLDAWAY-CLARKE, S. CORDEIRO, J.G. KUNKEL,P.K. HEPLER (2001) Cellular oscillations and the regulation of growth: thepollen tube paradigm. BioEssays 23(1):86-94(64) CARDENAS, L., T. HOLDAWAY-CLARKE, F. SANCHEZ, C. QUINTO, J. A. FEIJÓ,J. G. KUNKEL, P. K. HEPLER (2000) Ion changes in legume root hairsresponding to Nod factors. Plant Physiology 123:443-452(65) FEIJÓ, J.A., R. MALHÓ e G. OBERMEYER (1995) “Ion dynamics and itspossible role during in vitro pollen germination and tube growth”. Protoplasma187:155-167Invited Book Chapters(66) Prado, MP, Feijó, JA, Porterfield, MD (2010) Calcium dependent NOsignaling in plant cell polarity and sexual reproduction. Nitric Oxide in PlantPhysiology. S. Hayat, M. Mori, J. Pichtel, and I. Ahmad (eds.) WILEY-VCHVerlag GmbH & Co. KGaA, Weinheimin. pp. 31-50(67) Cruz, C, Ramos, AC, Façanha, A, Feijó, JA, Martins-Loução, MA (2009)Enzyme activity modulated by AMF colonization: the urea cycle andmembrane-bound phosphatase activities. In Varma A , Verma E (eds.)Microbes for human life, Springer-Verlag, ch25, 10 pp(68) Ramos, AC, Façanha, AR, Feijó JA (2008) Ion dynamics during thepolarized growth of arbuscular mycorrhizal fungi: from presymbiosis tosymbiosis. In Varma, A (ed.), MYCORRHIZA: Structure Function andBiotechnology, Springer-Verlag, Heidelberg. pp.214-260(69) Moreno N, Colaço R, Feijó JA (2007) The pollen tube oscillator: integratingbiophysics and biochemistry into cellular growth and morphogenesis. InMancuso, S, and Shabala, S (eds.) Rhythms in Plants: Phenomenology,Mechanisms, and Adaptive Significance,Springer-Verlag,Heidelberg, pp.39-62(70) Moreno, N, Bougourd, S, Haseloff, J, Feijó, JA (2006) Imaging Plant Cells.in J.Pawley (ed.) Handbook of Biological Confocal Microscopy, 3 rd . edition,ch.44, Springer, NY, pp. 769-787. Cover image

(71) Kunkel, J.G., Cordeiro, S., Xu, J., Shipley, A.M. and Feijó, J.A. (2006) Theuse of non-invasive ion-selective microelectrode techniques for the study ofplant development. In Volkov, V. (ed.) Plant Electrophysiology – Theory andMethods, Springer-Verlag, Berlin, Heidelberg, pp. 109-137(72) Kunkel, J.G., L.-Y. Linx , Y. Xu, A. M. M. Prado, J. A. FEIJÓ, P. P. Hwangxand P. K. Hepler. (2001) The strategic use of Good buffers to measure protongradients about growing pollen tubes. In Geitmann, A. et al. (eds.) Cell Biologyof Fungal and Tip Growth. NATO Science Series I:Life and Behavioural Sci.,Vol.328, IOS Press, Amsterdam, Netherlands, pp.81-94(73) FEIJÓ, J.A. (1999) The pollen tube oscillator: towards the molecularmechanism of tip growth?. In M.Cresti, G.Cai and S.Moscatelli (eds.)“Fertilization in Higher Plants: Molecular and Cytological Aspects”. Springer-Verlag. Heidelberg, Berlin. pp.317-336(74) SHIPLEY, A.M., J.A. FEIJÓ (1999) The use of the vibrating probe technique tostudy steady extracellular currents during pollen germination and tube growth.In M.Cresti, G.Cai and S.Moscatelli (eds.) “Fertilization in Higher Plants:Molecular and Cytological Aspects”. Springer-Verlag. Heidelberg, Berlin.pp.235.252(75) FEIJÓ, J.A., A.C. CERTAL, L. BOAVIDA, I. VAN NERUM, T. VALDIVIESSO,M.M. OLIVEIRA, W. BROOTHAERTS (1999) Advances on the study of sexualreproduction in the cork-tree (Quercus suber L.), chestnut (Castanea sativaMill.) and in Rosaceae (apple and almond). In M.Cresti, G.Cai andS.Moscatelli (eds.) “Fertilization in Higher Plants: Molecular and CytologicalAspects”. Springer-Verlag. Heidelberg, Berlin. pp.377-396(76) PAIS, M.S.S. e J.A. FEIJÓ (1991) "Some cytological aspects of pollenbiology in Ophrys lutea ". in G.Blanca et al. (eds.) Pollen, esporas y susaplicaciones, A.P.L.E., Granada, pp. 73-85(77) FEIJÓ, J.A. e M.S. PAIS (1990) "Rejuvenation of adult specimens ofCastanea sativa Mill. through in vitro micropropagation". in R.Rodriguez et al.(eds.) Plant aging, basic and applied aspects. NATO ASI series, series A (lifesciences). 186: 383-387Invited scientific seminars/ talks(2004-…; does not include pedagogic/ teaching seminars)(1) Mexico, Irapuato+Cuarnavaca, May 2013, seminar

(2) Intl Workshop Plant Membrane Biology, Kurashiki, Japan, March 2013,keynote speaker (http://www.iwpmb2013.jp/)(3) Hokaido University, Japan, March 2013, seminar(4) Pollen Network RCN-III, Tucson, US, March 2013, plenary conference(http://pollennetwork.org/?q=content/third-rcn-meeting-march-1-3-2013)(5) Symposium+Workshop - Frontiers in Pollen Tube Biology, Shangai, October2012, conference+practical seminar on methods to study ion regulation ofgrowth (http://www.iasprr.org/index.php?browse=news&nid=20)(6) 10 th Intl Congress Plant Mol Biol (ASPB) October 2012, Jeju Island, SouthKorea, keynote speaker (my.aspb.org/events/event_details.asp?id=147855)(7) InternationalCrop Genomics Conference, July 1-3, 2012, Wuhan (China) June2012, plenary conference(8) China Agric. Univ., Dept. Biosciences, Beijing, China, June 2012, seminar(9) China Academy of Sciences, Beijing, China, June 2012, seminar(10) 77 th Cold Spring Harbor Symposia- The Biology of Plants, May/June 2012,plenary speaker(11) Univ. Lausanne, CH, Dept Plant Biology, May, 2012, seminar(12) Univ. Zurich, Dept. Plant Genetics, CH, May 2012, seminar(13) Univ. Wurzburg, D, Bot Inst, March 2012, seminar(14) Univ. Cologne, D, PhD program, March 2012, seminar(15) Norwich, UK, John Innes RC, January 2012, seminar(16) U.Maryland, USA, 2011, Dept Cell and Molecular Biology, seminar(17) Wagenigen, 2011, “Botanical Microscopy Meeting” (under the auspices ofthe Royal Microscopical Society, http://www.rms.org.uk/) Plenary Speaker(18) Granada, Est.Experim.Zaidin, Seminar, Apr 2011(19) Biophysical Basis of Development, IGC-Oeiras, EMBO workshop, PlenarySpeaker, member of the scientific board, May 2011(20) Soc.Experimental Biology/UK, Glasgow, keynote speaker, July 2011(21) The Harold Woolhouse Lecture 2011 “Space and time coordination ofcellular growth processes by free cytosolic ions in pollen tubes” Univ.Adelaide, Australia, 1st August 2011(22) Cologne University/Max Planck Institute, Seminar, June 2011(23) Intl Botany Meeting, Melbourne, Australia, Symposium keynote speaker,chairman and co-organizer of the symposium on pollen-pistil interactions, July2011(24) “Of models and mechanisms of ion dynamics and signaling in pollen tubes “RCN pollen workshop; satellite meeting to the ASPB, Minnesota, August,2011

(25) “Excitement in sexual plant reproduction: the remarkable biology of pollentubes” Genetics Society, Coimbra, 2011(26) Univ. Bristol, IASPR, Augusto 2010, Plenary Speaker(27) Univ. Valencia, FESPB, July 2010, (www.fespb2010.org), Plenary Speaker(28) Agropolis Foundation, Montpellier, Imaging, phenotyping and modellingplant organ morphogenesis, Março 2010, Plenary Speaker(29) GARNet 2010 Meeting - Cells and Systems - Durham UK Monday 6 thSeptember, plenary speaker(30) 15 th International Workshop on Plant Membrane Biology, 19 th -24 thSeptember, 2010, plenary speaker(31) Norwich, John Ines Research Institute, Seminar, May 2010(32) Univ. Cambridge, Dept. Plant Sciences, UK, Pollen tube growth: of malegametophytes and their urges for models, Jan 2009(33) Univ. York, Biomechanics of Growth in Plant Biology, March 2009,(34) Plant Molecular Biology, St. Louis, Missouri, USA, Oct 2009, keynotespeaker(35) EMBO- Live Cell Imaging, IGC, Oeiras, May 2009. The perfect tool for theright experiment(36) Biophysical Basis of Development, IGC, Oeiras, May 2009.(37) Congresso Iberico Fisiologia Vegetal, Zaragoza, Setembro 2009, Plenaryspeaker, member of the scientific committee.(38) EMBO-Developmental Imaging, June, 2008, Plant Imaging(39) Biophysics Society, Lisboa, July, 2008, “Excitement in sexual plantreproduction: the remarkable biophysics of pollen tubes”(40) MBI- Columbus, Ohio, USA, September, 2008, “Excitement in sexual plantreproduction: the remarkable biology of pollen tubes and their urges formodels”(41) FEBS- Wagenigen, NL, September, 2008, Two-photon fluorescence in plantsciences.(42) FSPR III- Tucson, AZ, USA, October, 2008 Of male gametophytes and theirurges for models(43) Feijó, JA (2007) “Ions-in, ions-out, the way pollen tubes use water flow tounderlie growth and morphogenesis regulation”, Gordon Research Conferenceon Cellular Osmoregulation: Sensors, Transducers & Regulators, Aussois,França, Junho.(44) Feijó, JA (2007) “Plants, sex and pollen tubes: how evolution shapes specialcells for special purposes”. I Encontro Cientifico Multidisciplinar da Univ. deVigo, Espanha, Junho

(45) Feijó JA (2007) Member of the international scientific committee, SessionChairman, International Congress on Plant Membranes, Valencia, Espanha,Junho.(46) Feijó, JA (2007) “Live cell imaging methods: new tools and old tricks”.Advanced. Live Cell Microscopy Workshop, CNIO- Madrid, Espanha,Outubro(47) Feijó, JA (2007) “The control of apical cell growth and morphogenesis byion dynamics”, Génoscope/Génopole, Évry, Paris, France(48) Feijó JA (2006) “The control of apical cell growth and morphogenesis byion dynamics” Friday seminar lecture series, John Innes Research Center,Norwich, UK, Abril(49) Feijó JA (2006) “The control of apical cell growth and morphogenesis byion dynamics”, Genetics Society- Arabidopsis Meeting, Univ. Cambridge,UK, Maio(50) Feijó, J.A. (2006) “Live cell imaging methods: new tools and old tricks”CNIO-Leica Advanced. Live Cell Microscopy Workshop, Madrid, Espanha,Junho 2006(51) Feijó, JA (2006) “The control of apical cell growth and morphogenesis byion dynamics” IGC, Setembro 2006(52) Feijó, JA (2006) “The control of apical cell growth and morphogenesis byion dynamics”, seminar at Dept. of Molecular Genetics, Univ. Utrecht,Setembro 2006(53) Feijó, JA (2006) “Two-photon fluorescence in plant sciences” FEBS Courseon Microspectroscopy, Univ. Wagenigen, Holanda, Setembro de 2006(54) Feijó JA (2005) “The control of apical cell growth and morphogenesis byion dynamics”, Padova, 6 Junho.(55) Feijó JA (2005) "Live cell imaging methods: new tools and old tricks",Pleanry lecture for the course “L'IMAGING DALLA CELLULA ALLACOSCIENZA”, PhD. Program on pharmacology, chemotherapy andtoxicology, UNIVERSITÀ DI MILANO, ISTITUTO DI NEUROSCIENZE, Milano, Itália, 8Junho(56) Feijó JA (2005) “The control of apical cell growth and morphogenesis byion dynamics” Siena, 10 June.(57) Feijó JA (2005) “The control of apical cell growth and morphogenesis byion dynamics”, keynote lecture on the session “apical growth”, XVIIInternational Botanical Congress, Viena, Julho(58) Feijó JA (2005) “The control of apical cell growth and morphogenesis byion dynamics” Soc.Exp.Biol. meeting on Plant Channels, Glasgow, Setembro

(59) Feijó JA (2005) “The control of apical cell growth and morphogenesis byion dynamics” Conferência por convite no Iberian Meeting of the Spanish andPortuguese Societies of Plant Physiology, Évora, Setembro(60) Feijó JA (2005) “The control of apical cell growth and morphogenesis byion dynamics” Seminar, Univ. Wagenigen, Holanda, Setembro(61) Feijó JA (2004) Seminar “1st. GABBA workshop”, IBMC, Porto(62) Feijó JA (2004) Plenary lecture. Reunião Anual da Soc. BioquímicaBrasileira, Maio, Caxambu, Brasil(63) Feijó JA (2004) Opening plenary lecture and Chairman of the session “ionsand development”, International Congress on Plant Membranes, July,Montpellier, França(64) Feijó JA (2004) Plenary lecture and Chairman of the session “Advancedapproaches to SPR”. Frontiers on Sexual Plant Reproduction, Outubro,Albany, USA(65) Feijó JA (2004) Biology Seminar Series. PennState Univ, USA(66) Feijó JA (2004) Plant Biology Graduate Program Seminar, U.Massachussets,Amhesrt, USA(67) Feijó JA (2004) Plenary lecture. FEBS course on microspectromics,Outubro, Wagenigen, Holanda(68) Feijó JA (2004) Plenary lecture, Chairman and member of the scientificcommittee. Reunião da Soc. Portuguesa de Bioquímica, Novembro,VilamouraGrant reviewing-Agence Nationale de Recherche- Programme Plates-formes Technologiques duVivant, 2007, France-Austrian Science Fund (FWF)-BARD- United States- Israel Binational Agricultural Research & DevelopmentFund (Israel)-BBRSC- British Biotechnology Research and Scientific Council (UK)-BMBF (German Federal Ministry of Education and Research); GABI-FUTURE-Conselho de Reitores das Universidades Portuguesas (CRUP-Portugal)-European Community- ERANET-PG (Plant Genomics) 2006- SCIENTIFIC BOARDMEMBER-ERC- European Research Council: scientific evaluation panel member for startinggrants (Jan-May 2013)(top-level research council in the EU)

-Fundação para a Ciência Tecnologia (FCT- Portugal)-Grant Agency, Academy of Sciences of the Czech Republic-Natural Sciences and Engineering Research Council of Canada(NSERC)-NSF- National Science Foundation (USA)-NWO- Nederlandse Organisatie voor Wetenschappelijk Onderzoek (Netherlands)-Research Foundation Flanders (FWO)(Belgium)-USDA- U.S. Dept. Agriculture (USA)Tenure-track or Full-professorship external evaluation-2009- Brown University, Dept. Mol.Cell Biology-2011- Arizona State University (Tucson), Dept. Plant Biology-2012- University of Massachusetts (Amherst), Dept.Biochem.Mol.Biology“Ad-hoc” Peer-Reviewing-Advances in Botanical Research (Elsevier)-Annals of Botany (Pergamon)-Annals of Forestry Science (INRA-EDP Sciences)-Australian Journal of Botany (CSIRO)-BBA- Molecular Cell Research (Elsevier)-BioEssays (John Wyley)-Biophysical Journal (Cell Press- The Biophysical Society)-BMC Biology (BioMed Central)-BMC Plant Biology (BioMed Central)-Bulletin of Mathematical Biology (Springer)-Critique Internationale (Ed. SceancesPo, Paris)-Development (The Company of Biologists)-Environmental and Experimental Botany (Elsevier)-EvoDevo (BioMed Central)-Euphytica (Springer)-FEBS Letters (Elsevier)-FEMS Microbiology Reviews (Fed.Eur.Microb.Soc.)-Frontiers in Plant Physiology (www.frontiers.org)-Functional & Integrative Genomics (Springer)-Genes and Development (Cold Spring Harbor Lab. Press)

-HFSP Journal (Human Frontiers Science Program Society)-International Journal of Developmental Biology (Univ. Basque Country)-Journal of Biology (BioMed Central)-Journal of Cell Science (The Company of Biologists)-Journal of Experimental Botany (Oxford Univ. Press)-Journal of Integrative Plant Biology (Chinese Academic of Sciences)-Journal of Plant Physiology (Elsevier)-Journal of the Royal Society Interface (Royal Soc. London)-Molecular Biology Reports (Springer)-Molecular Plant (Oxford Journals)-Journal of Proteome Research (American Chemical Society)-Nature Communications (NPG)-Nature Reviews on Molecular and Cellular Biology (NPG)-NATO Science Series (Elsevier)-Organic & Biomolecular Chemistry (Royal Soc. Chemistry)-Physiologia Plantarum (Blacwell Pub.Co.)-Plant and Cell Physiology (Oxford Univ. Press/ Jap. Soc. Plant Physiol.)-Plant and Soil (Springer)-Plant Biology (Thieme)-Plant Journal (Blackwell Pub.Co.)-Plant Physiology (American Society of Plant Biologists)-Plant Breeding (Springer-Verlag)-Plant Molecular Biology (Springer)-Plant Science (Elsevier)-Planta (Springer-Verlag)-PLOS Biology (PLOS)-PLoS Genetics (PLOS)-PLOS One (PLOS)-Proceedings of the Natural Academy of Sciences USA (Natl.Acad.Sci. USA)-Protoplasma (Springer)-Science (American Association for the Advancement of Science)-Sexual Plant Reproduction (Springer-Verlag)-The New Phytologist (The New Phytologist Trust)-The Plant Cell (American Society of Plant Biologists)-Trees (Springer-Verlag)-Trends in Cell Biology (Elsevier)-Trends in Plant Science (Elsevier)

Editorial Activity:-Sexual Plant Reproduction (Editorial Board) (Springer-Verlag); Fev 2005-2012-Biology Image Library (Editorial Board- Plant Biology/Cell Biology;http://images.biomedcentral.com/editorial/#4937); 2005-…-Frontiers in Plant Physiology (Review Editor) (www.frontiers.org); 2011-…-Frontiers in Plant Biophysics. and Modeling (Review Editor) (www.frontiers.org);2011-…-Molecular Plant (Editorial Board)(Oxford Univ.Press); 2011-…Prizes and other distinctions- Best Master Thesis, 1989, Portuguese Society of Biotechnology- National Forestry Prize, 1992- Fellow of the Linnean Society of London (2009-…)