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VALIDATION REPORT-IDEXX PRRS OF (IDEXX and UMN-VDL)Figure 1 shows the measurement discrimination plots and intra-class correlation coefficients (r icc ) for thepanel testing at IDEXX. Each of the kit pairs demonstrated an r icc value above 0.99, which indicates ahigh degree of correlation.Figure 1: Kit-kit agreement with the 20-sample panel- IDEXX Test ResultsUniversity of Minnesota Veterinary Diagnostic Laboratory (UMN-VDL) – 20-sample panelTable 2 shows sample ID, sample status, and IDEXX PRRS OF test results from testing of IDEXX 20-sample panel at the UMN-VDL. Categorical agreement with sample status for all three kits was 100%.Table 2: UMN-VDL Testing of PRRS OF Field Trial 20-sample panelIDEXX Panel A MJF-00890 SIK-0828 SIK-0843Rep 1 Rep 2 Ave Rep 1 Rep 2 Ave Rep 1 Rep 2 AveNeg 0.039 0.035 0.037 Neg 0.037 0.034 0.036 Neg 0.029 0.031 0.030Pos 0.618 0.637 0.628 Pos 0.64 0.644 0.642 Pos 0.664 0.658 0.661Sample SampleSample-NCSample-NCSample-NCODS/P Result ODS/P Result ODID statusODODODS/P Result1 Neg 0.036 -0.001 -0.002 Neg 0.040 0.004 0.006 Neg 0.033 0.003 0.005 Neg20 Neg 0.040 0.003 0.005 Neg 0.037 0.001 0.002 Neg 0.041 0.011 0.017 Neg13 Neg 0.035 -0.002 -0.003 Neg 0.032 -0.004 -0.006 Neg 0.036 0.006 0.009 Neg15 Neg 0.033 -0.004 -0.006 Neg 0.028 -0.008 -0.012 Neg 0.033 0.003 0.005 Neg14 Neg 0.033 -0.004 -0.006 Neg 0.028 -0.008 -0.012 Neg 0.032 0.002 0.003 Neg17 Neg 0.031 -0.006 -0.010 Neg 0.028 -0.008 -0.012 Neg 0.034 0.004 0.006 Neg9 Neg 0.025 -0.012 -0.019 Neg 0.019 -0.017 -0.026 Neg 0.015 -0.015 -0.023 Neg12 Neg 0.025 -0.012 -0.019 Neg 0.018 -0.018 -0.028 Neg 0.017 -0.013 -0.020 Neg3 Pos 0.386 0.349 0.556 Pos 0.535 0.499 0.777 Pos 0.439 0.409 0.619 Pos11 Pos 0.384 0.347 0.553 Pos 0.521 0.485 0.755 Pos 0.435 0.405 0.613 Pos2 Pos 0.629 0.592 0.943 Pos 0.670 0.634 0.988 Pos 0.689 0.659 0.997 Pos10 Pos 0.608 0.571 0.909 Pos 0.658 0.622 0.969 Pos 0.691 0.661 1.000 Pos5 Pos 0.903 0.866 1.379 Pos 1.015 0.979 1.525 Pos 1.111 1.081 1.635 Pos8 Pos 0.898 0.861 1.371 Pos 0.961 0.925 1.441 Pos 1.045 1.015 1.536 Pos16 Pos 1.416 1.379 2.196 Pos 1.502 1.466 2.283 Pos 1.722 1.692 2.560 Pos19 Pos 1.570 1.533 2.441 Pos 1.501 1.465 2.282 Pos 1.839 1.809 2.737 Pos7 Pos 1.812 1.775 2.826 Pos 2.114 2.078 3.237 Pos 2.116 2.086 3.156 Pos18 Pos 1.856 1.819 2.896 Pos 2.084 2.048 3.190 Pos 2.225 2.195 3.321 Pos4 Pos 3.102 3.065 4.881 Pos 2.947 2.911 4.534 Pos 3.084 3.054 4.620 Pos6 Pos 2.995 2.958 4.710 Pos 2.944 2.908 4.530 Pos 3.406 3.376 5.107 PosPrinted: 3/13/2013 Page 2 of 4