PRP-X100 Anion Exchange HPLC Columns 4/3/2007 - Western ...

Hamilton HPLC - PRP-X100 Anion Exchange HPLC ColumnsPage 1 of 44/3/2007U S ANew ApplicationsHPLC Application IndexOrdering InformationContact HPLC SupportPolymeric anion exchange packing for separation ofinorganic and organic anions from 10 to 500 ppm:• Easily separate the eight common anions(fluoride through sulfate)• Good separation of fluoride from the water dip• Use with organic solvent from 0 to 100% for elution of hydrophobicanions or column cleaning.• pH stable from 0 to 14.• Use conductivity or UV detection.Product Features• Four particle sizes: 3, 5, 10 and 12-20um.• Eight column internal diameters: 1.0 to 50.8 mm.• Two column materials: 316 stainless steel and PEEK.• Analytical and semiprep/preparative guard columns.What Types of Samples Can PRP-X100 Columns Separate?Common Anions by Conductivity DetectionPRP-X100, 4.1 x 50mm, 3um (P/N 79810) 1. Fluoride2. Chloride3. Nitrite4. Bromide5. NitrateConditions: 4mM p-Hydroxybenzoic Acid pH 8.5w/2.5% MethanolFlow: 1 mL/min.Injection: 100 µLDetection: ConductivityCompatibilityThese columns can be used to determine ions with regular HPLC's and dedicated ion chromatographs.Eight Anions by Indirect UV DetectionPRP-X100, 4.1 x 150mm, 10um (P/N 79434)1. Fluoride 10ppm2. Carbonate 10ppm3. Chloride 10ppm4. Nitrite 10ppm5. Bromide 10ppm6. Nitrate 10ppm7. Phosphate 10ppm8. Sulfate 10ppmConditions: 4mM p-Hydroxybenzoic Acid pH 8.9with 2.5% Methanol. Isocratic. Ambient.Flow:2 mL/min.Injection: 100 µLDetection: Indirect UV 310nm.EDTAPRP-X100, 4.6 x 150mm, 10um (P/N 79354)1. Copper EDTA 10ppmConditions: 3mM Sulfuric AcidIsocratic. Ambient.Flow:2 mL/min.Injection: 100 µLDetection: UV 254nm.

Hamilton HPLC - PRP-X100 Anion Exchange HPLC ColumnsPage 2 of 44/3/2007Organic AcidsPRP-X100, 4.1 x 150mm, 10um (P/N 79434)1. Formic Acid 10 ug2. Fluoroacetic Acid 10 ug3. Isopropylmethyl Phosphonic Acid 10 ug4. Chloroacetic Acid 10 ugConditions: 0.1M Sodium Hydroxidein 5:95 Acetonitrile : Deionized Water.Isocratic. Ambient.Flow:2 mL/min.Injection: 10uLDetection: Conductivity.DetectionFor customers with HPLC instrumentation conductivity and ultraviolet/visible (UV/Vis) detection can be used for anion analysis.The dedicated ion chromatographer can use either chemically suppressed or nonsuppressed detection. PRP-X100 columns can be used by both customers.Germall 115PRP-X100, 4.6 x 150mm, 10um (P/N 79354)1. Germall 115Conditions:7:3 20mM Sodium Hydroxide : Methanol.Isocratic. Ambient.Flow: 2 mL/min.Injection: 50uLDetection: UV 220nm.Bromate and Other AnionsPRP-X100, 4.1 x 150mm, 10um (P/N 79434)1. Fluoride 20ppm2. Chloride 20ppm3. Bromate 40ppm4. Bromide 20ppm5. Nitrate 20ppmConditions: 1.7mM Sodium Bicarbonate,1.8mM Sodium Carbonate, 0.1mM SodiumThiocyanate. Isocratic. Ambient.Flow: 2 mL/min.Injection: 50uLDetection: UV 220nm.Sulfite and SulfatePRP-X100, 4.6 x 150mm, 10um (P/N 79354)1.Sulfite 1.27mg/mL2. Sulfate 0.5mg/mLConditions: 80:20 8mM p-HydroxybenzoicAcid pH 10.0 : Methanol. Isocratic. Ambient.

Hamilton HPLC - PRP-X100 Anion Exchange HPLC ColumnsPage 3 of 44/3/2007Flow: 2 mL/min.Injection: 10uLDetection: ConductivityLong Column LifeThe highly inert polymeric support resists chemical attack from organic solvents and aqueous buffers (0-100% aqueous or organic; pH 0 to 14), effectivelylengthening column life. If column performance should deteriorate (peak broadening and a loss of symmetry), the regeneration protocol will usually returnyour chromatography to its original state.Phosphite, Phosphateand Other AnionsPRP-X100, 4.1 x 150mm, 10um (P/N 79434)1. Fluoride 10ppm2. Phosphate 50ppm3. Phosphite 50ppm4. Chloride 10ppmConditions: 20mM Succinic Acid pH 2.9.Isocratic. Ambient.Flow: 2 mL/min.Injection: 100uLDetection: ConductivityChlorite, Chlorate and Other AnionsPRP-X100, 4.1 x 250mm, 10um (P/N 79433)1. Fluoride2. Chlorite3. Chloride4. Nitrite5. Phosphate6. Bromide7. Nitrate8. ChlorateConditions: 4.0mM 4-Amino-2-HydroxybenzoicAcid pH 5.8. Isocratic. Ambient.Flow: 2 mL/min.Injection: 100uLDetection: Indirect UV 310nmAnalyze Both Difficult and Common AnionsPRP-X100 columns easily separate difficult anions such as cyanide, borate and silicate at high pH (11.5). The polymeric packing is stable from pH 0 to 14, so asingle column can be used for the analysis of both common and difficult anions.Anions at pH 11.5250 x 4.1 mm PRP-X100 (P/N 79433)1. Borate 50 ppm2. Fluoride 50 ppm3. Silicate 50 ppmConditions: 3.2 mM Potassium Hydroxide pH 11.5 Isocratic. Ambient.Flow: 3.0 mL/min.Injection: 100 µLDetection: Conductivity

Hamilton HPLC - PRP-X100 Anion Exchange HPLC ColumnsPage 4 of 44/3/2007NEW APPLICATIONS#294 EDTA in Cold Capsules as Iron EDTA#372 EDTA, Glutathione, Citric Acid#379 EDTA as Copper Complex#300 EDTA, NTA, DTPA#287 EDTA in EYE Drops asCopper Complex#465 Arsenic SpeciationORDERING INFORMATIONPRP-X100 Anion Exchange HPLC ColumnsParticle SizeI.D. x Length 5 µm 10 µm 12-20 µm1.0 x 50 mm 79767 797711.0 x 100 mm 79768 797721.0 x 150 mm 79769 797732.1 x 50 mm 79150 797492.1 x 150 mm 794212.1 x 150 mm* 79852 798532.1 x 250 mm 793462.1 x 250 mm* 791484.1 x 50 mm 793654.1 x 100 mm 79538 794394.1 x 125 mm 795614.1 x 150 mm 794344.1 x 250 mm 79433 793594.6 x 150 mm* 79174 793544.6 x 250 mm* 79181 794557.0 x 305 mm 7936410.0 x 150 mm 7971510.0 x 250 mm 7953421.5 x 100 mm 7954321.5 x 150 mm 7954221.5 x 250 mm 79535 7935350.8 x 150 mm 7955150.8 x 250 mm 79550* PEEK HardwarePRP-X100 Guard ColumnsFor Steel ColumnsFor PEEK ColumnsAnalytical Starter Kits (1 holder, 2 cartridges) 79448 79383Analytical Replacement Cartridges (5/pk) 79446 79385Semiprep/Preparative Starter Kit (1 holder, 1 cartridge) 79125Semiprep/Preparative Replacement Cartridges (2/pk) 79126Guard Column Technical InformationColumn Care and StorageAbout Hamilton Contact Quick Order Service News & PromosCopyright 1998-2004 Hamilton Company. All Rights Reserved.Terms of Use. Privacy Statement.