Abstract Book - Pathology and Laboratory Medicine - University of ...
Abstract Book - Pathology and Laboratory Medicine - University of ...
Abstract Book - Pathology and Laboratory Medicine - University of ...
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ABSTRACT #42CLINICAL SCIENCESGRADUATE STUDENTPOSTER presentationOral epithelial cell telomere lengths (TL) are correlated with peripheral bloodleukocyte TL in HIV+ <strong>and</strong> HIV-exposed uninfected (HEU) children, <strong>and</strong> are notaffected by HIV viremiaDeAnna Zanet 1 , Hugo Soudeyns 3 , Ariane Alimenti 4 , Valérie Lamarre 3 , Norm<strong>and</strong> Lapointe 3 , JasonBrophy 6,7 , Lindy Samson 6,7 , Deborah M Money 1,2,4 , Anona Thorne 5 ; John C Forbes 4 , Hélène CF Côté 1,2<strong>and</strong> the CIHR Emerging Team in HIV therapy <strong>and</strong> aging (CARMA)1UBC; 2 WHRI; 3 Centre de Recherche du CHU Sainte-Justine, Montreal 4 B.C. Women’s Hospital; 5 CIHRCanadian HIV Trials Network; 6 Children’s Hospital <strong>of</strong> Eastern Ontario; 7 <strong>University</strong> <strong>of</strong> Ottawa54Background /objectivesTelomeres cap <strong>and</strong> protect the ends <strong>of</strong> chromosomes, <strong>and</strong> shorten with each somatic cell division, acting as amarker <strong>of</strong> aging. Telomerase, homologous to HIV reverse transcriptase, is expressed in germ stem <strong>and</strong> stem cells,<strong>and</strong> maintains telomere length (TL). Nucleoside reverse transcriptase inhibitors (NRTIs) are given to HIV+pregnant women for their own health <strong>and</strong> to prevent mother to child transmission <strong>of</strong> the virus. HIV-exposeduninfected (HEU) infants also receive NRTI prophylaxis for 6 weeks after birth. NRTI inhibition <strong>of</strong> telomerase<strong>and</strong> HIV-induced inflammation <strong>and</strong>/or oxidative stress can adversely affect TL maintenance, <strong>and</strong> possiblyinfluence tissue aging. We previously showed that although peripheral blood leukocyte (PBL) TL was similarbetween HEU <strong>and</strong> HIV+ children, the latter demonstrated shorter TL if they had a detectable HIV plasma viralload. This study examined whether similar TL dynamics are observed in buccal epithelial cells.MethodsHIV+ <strong>and</strong> HEU children were prospectively enrolled in the CARMA cohort. Mouth swabs <strong>and</strong> PB werecollected from each child <strong>and</strong> their relative TL was measured by qPCR. Univariate linear regressions examinedrelationships <strong>of</strong> potential predictor variables with TL. Three multivariate models were created: one for allsubjects, <strong>and</strong> two others that included HIV+ <strong>and</strong> HEU subjects only.ResultsOral epithelial cell (OEC) TL data was obtained for 90 HIV+ (median [IQR] age (years) 13.1 [9.7-15.6]) <strong>and</strong>231 HEU (1.6 [0.5-3.3]) children. All HEU children were exposed to NRTI in utero <strong>and</strong>/or neonatally. OECTL was ~70% longer (p
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