The Adventures of AntiBobby - Thomson Instrument Company

We Start with many Ideas or Variables of DNAAntiBobbyOur DNA AntiBobby gets wrapped in aBLANK Ecoli cell. Kind of like ajacket!1 2The clones of AntiBobbyWhat Happens in the Mega-Prep® Column?5AntiBobby is placed into a Thomson Ultraget pushed through aYield Flask and placed in a shaker. FlaskMega-Prep® Column andsize is determined by the size of theirlike a food processor itexperiment, over a day AntiBobby grows manyunwraps the Ecoli jacketmany clones of himself.and cleans any impurities, sowe have only the AntiBobbyDNA. And thanks toThomson Products we haveLOTS to work with!Now we can put AntiBobbyChemicals are added to the Column thatinto a Mammalian or Insectbreak up the jacket around AntiBobby.Jacket.Now you have AntiBobby and all of theEcoli jacket pieces.62.5L Ultra Yield FlaskPlasmid+ Media!Inside the column the Ecoli jacketpieces get stuck to the columnresin. When they push a rinseagent through only AntiBobby34Thomson Instrument Company is not affiliated with Qiagen or their products.exits the column.

But if we wanted AntiBobby in aMammalian or Insect cell, why didn‛t wejust start the process in the cell jacketwe wanted?Well.. It‛s based on a principle called doubling. Yousee an Ecoli jacket is thicker walled so we canagitate AntiBobby faster without hurting him. AndEcoli‛s doubling rate is 2 hours, so every Two hoursour amount of AntiBobbys doubles.8As apposed to our mammalian and insect cell walls thatare thinner and so we have to agitate the growth at aslower rate. The doubling rate is 24 hours! So yousee that is why we start the process with Ecoli to geta large batch to play with. Then start puttingAntiBobby into our Mammalian and insect cells.7But how do we do that?9Labs create reservoirs of BLANK Mammalian & Insect cells towrap around only the BEST AntiBobbys.Now to get AntiBobby into an insector mammalian cell!12Reservoirs ofMammalian& Insect cellsFirst we coat AntiBobby in PEI or Liptofectin. Thismakes AntiBobby invisible to the host cell. This allowsAntiBobby to enter the cell and pass all of its securityguards to the nucleus.10To get AntiBobby into a mammalian or insect cellfirst we need to find the best AntiBobby, To dothat we use Elisa Testing. Basicaly we putAntiBobby up against a sample and see if he isidentical.11AntiBobby sneaking intomammalian or insect cellundetected!AntiBobby inside mammalian or insect cell and hemade it into the nucleus to implant our DNA intothe cell.Now we just have to grow more of these withincreasingly larger batches of AntiBobby and useElisa Testing to determine which batch goes on thethe next larger batch.

13Every time you grow DNA in batches you get some GOODbatches, some BAD ones, & some are the BEST of the BEST ofthe BEST in DNA batches.Batches are scored on concentration of DNA. We use the ElisaTesting to take a look at the batch and determine if it containsour AntiBobby or not and how much. Only the best batchesare used to grow the next larger batches.So to get a final product of our AntiBobby in a Mammalian orInsect jacket and ONLY have the BEST samples we have tostart small...Starting small means using a Thomson 24 (P/N 931565-1X) or 96 (P/N982090) well plate.We take the BEST ofthe BEST of the BESTfrom the Thomson WellPlate...We grow a small batch of AntiBobby in his new mammalian or insectjacket and see which well has the greatest concentration of AntiBobbyby using Elisa Testing.From there it is just repeating the same process in a larger then largercontainer. Eventually we get to a production run. But by then we wantto make sure that it is going to produce a high concentration ofAntiBobby so we start small and end with the best of thebest of the best in batches to grow with.1516...And start the process and growAntiBobby in 64 Thomson 125mL OptimumGrowth Flasks. Then use Elisa testingto determine which flask has the highestconcentration of AntiBobby and go larger!14

17 18 2019...36x...24x...16x...Production250mL...500mL...2.5 L...8 x 5 L Flasks...16 x 2.5L ThomsonOptimum GrowthFlasks... Elisa Testing...You‛re starting to get theidea by now how we getNext up!Thomson 250mL OptimumGrowth Flasks & 36 of themto get a good amount of cellslarge batches of goodAntiBobby aren‛t you.Well guess what?PRODUCTION! We have a STRONG strain ofAntiBobby in a mammanilan or insect cell and wehave 8 x 2.5L Flasks of him.and Elisa testing to get to thenext batch...We now have 16 x 2.5LFlasks with AntiBobby inmammalian or insect celljackets and we‛ll take thebest 8 and go to aproduction run...So production is produced in 8 Thomson 5L OptimumGrowth Flasks. 2 Flasks = 1 Wave Bag® and youcan fit 7 Flasks in an ATR shaker. That means thatin one ATR shaker you can produce 17.5L of productinstead of the 10L production in a 20L Wave Bag®.If you‛re keeping track that‛s an increase inproduction of 55%!...24 x 500mLThomson OptimumGrowth Flasks...Elisa Testing... andthe Best get sentdown the line to...