Microfluidic single-cell real-time PCR for comparative analysis of ...

protocolFigure 5 | Interpretation of heat map results.The heat map shows C Tvalues encoded bydifferent colors. Positive controls will showresults for all the studied genes, whereasnegative controls will not show any C Tvalue(encoded in black). Good samples will havehigh C Tvalues for the housekeeping gene,whereas a bad sample or chip run will notshow housekeeping gene C Tvalues or thesewill be contradictory. Among the goodsamples, there will be genes that are highlyexpressed compared with others that arebarely detected. It is also possible thatC Tvalues are not obtained for a specificgene, which can be interpreted as a lack ofexpression in this particular cell sample.Samples25 : S2526 : S2640 : S4032 : S3247 : S47Gene assays48 : A4847 : A4746 : A4645 : A4544 : A4443 : A4342 : A4241 : A4140 : A4039 : A3938 : A3837 : A3736 : A3635 : A3534 : A3433 : A3332 : A3231 : A3130 : A3029 : A2928 : A2827 : A2726 : A2625 : A2524 : A2423 : A2322 : A2221 : A2120 : A2019 : A1918 : A1817 : A1716 : A1615 : A1514 : A1413 : A1312 : A1211 : A1110 : A1009 : A0908 : A0807 : A0706 : A0605 : A0504 : A0403 : A0302 : A0201 : A01Housekeeping genein triplicatePositive controlNegative controlBad sample OR Chip runSample with C T values for all the genesSample with some undertermined genesUndeterminedgeneHighgene expressionVery lowgene expression© 2012 Nature America, Inc. All rights reserved.Acknowledgments We are grateful to P.E. de Almeida for FACS discussion.We acknowledge funding support from the Swiss National Science FoundationPBBEP3_129803 (V.S.-F.); the German Research Foundation (A.D.E.); the HowardHughes Medical Institute (S.R.Q.); the US National Institutes of Health (NIH)DP2OD004437, RC1AG036142, R01AI085575 (J.C.W.), and the Burroughs WellcomeFoundation (J.C.W.).AUTHOR CONTRIBUTIONS V.S.-F. and A.D.E. prepared most of the paper. T.K.,S.R.Q. and J.C.W. provided advice and proofread the paper.COMPETING FINANCIAL INTERESTS The authors declare competing financialinterests: details accompany the full-text HTML version of the paper at http://www.natureprotocols.com/.Published online at http://www.natureprotocols.com/.Reprints and permissions information is available online at http://www.nature.com/reprints/index.html.1. Kalisky, T. & Quake, S.R. Single-cell genomics. Nat. Methods 8, 311–314(2011).2. 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