ab112114 Calcium Assay Kit (Luminometric) - Abcam
ab112114 Calcium Assay Kit (Luminometric) - Abcam
ab112114 Calcium Assay Kit (Luminometric) - Abcam
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
<strong>ab112114</strong> <strong>Calcium</strong> <strong>Assay</strong> <strong>Kit</strong> -<strong>Luminometric</strong>8. TroubleshootingProblem Reason Solution<strong>Assay</strong> notworkingUnexpectedresults<strong>Assay</strong> buffer atwrong temperatureProtocol step missedPlate read atincorrect wavelengthUnsuitable microtiterplate for assayMeasured at wrongwavelengthSamples containimpeding substancesUnsuitable sampletypeSample readings areoutside linear range<strong>Assay</strong> buffer must not be chilled- needs to be at RTRe-read and follow the protocolexactlyEnsure you are usingappropriate reader and filtersettings (refer to datasheet)Fluorescence: Black plates(clear bottoms);Luminescence: White plates;Colorimetry: Clear plates.If critical, datasheet will indicatewhether to use flat- or U-shapedwellsUse appropriate reader and filtersettings described in datasheetTroubleshoot and also considerdeproteinizing samplesUse recommended samplestypes as listed on the datasheetConcentrate/ dilute samples tobe in linear range11
Change language