ab112114 Calcium Assay Kit (Luminometric) - Abcam

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ab112114 Calcium Assay Kit -Luminometric2. Protocol SummarySummary for One 96-well Plate..Prepare cellsRemove growth mediumAdd coelenterazine-loading solution(100 µL /well/96-well plate or25 µL/well/384-well plate)Incubate at room temperature for 3 - 4 hours.Monitor aequorin luminescence intensityNote: Thaw all the kit components to room temperature beforestarting the experiment.5
3. Kit Contentsab112114 Calcium Assay Kit -LuminometricComponentsAmountComponent A: Coelenterazine Analog1 vial (lyophilized)Component B: 100% ETOH 1 x 500 µLComponent C: Assay Buffer100 mL (1X ready to use)4. Additional Materials Required• 96 or 384 – well microplates: Tissue culture microplateswith white wall and clear bottom• Luminecence microplate reader• HHBS (1X Hank’s with 20 mM Hepes Buffer, pH 7.0)5. Storage and HandlingKeep at -20°C. Avoid exposure to light.6
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Page 5: Kit Key Featuresab112114 Calcium As

ab112114 Calcium Assay Kit (Luminometric) - Abcam

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