ab112114 Calcium Assay Kit (Luminometric) - Abcam
ab112114 Calcium Assay Kit (Luminometric) - Abcam
ab112114 Calcium Assay Kit (Luminometric) - Abcam
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<strong>ab112114</strong> <strong>Calcium</strong> <strong>Assay</strong> <strong>Kit</strong> -<strong>Luminometric</strong>Problem Reason SolutionStandardcurve is notlinearNot fully thawed kitcomponentsPipetting errors whensetting up thestandard curveIncorrect pipettingwhen preparing thereaction mixAir bubbles in wellsConcentration ofstandard stockincorrectErrors in standardcurve calculationsUse of otherreagents than thoseprovided with the kitWait for components to thawcompletely and gently mix prioruseTry not to pipette too smallvolumesAlways prepare a master mixAir bubbles will interfere withreadings; try to avoid producingair bubbles and always removebubbles prior to reading platesRecheck datasheet forrecommended concentrations ofstandard stocksRefer to datasheet and re-checkthe calculationsUse fresh components from thesame kitFor further technical questions please do not hesitate tocontact us by email (technical@abcam.com) or phone (select“contact us” on www.abcam.com for the phone number foryour region).13
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