PALM Applications Focus: Plant Sciences
PALM Applications Focus: Plant Sciences
PALM Applications Focus: Plant Sciences
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<strong>PALM</strong> <strong>Applications</strong><strong>Focus</strong>: <strong>Plant</strong> <strong>Sciences</strong>Laser Microdissection & Pressure Catapulting of Z. Mais Tapetal Cell Anthersa) b)Zea mais anthers horizontal section, mounted onregular glass slides. Laser microdissection of thetapetum.a), b) Drawing tools in <strong>PALM</strong> software outlinedesired cells (solid line) and set LPC sites (dots).d)c), d) Section after catapulting (with and withoutdrawing, respectively)e) Molecular analysis of RNA harvested from 5,10, 25 and 50 tapetal cells. RP: Rf2a RNApositive control, GP: Rf2a genomic DNA positivecontrol, N: Negative control for PCR, Rf2a primerpair: RF2C1 and RF2C2(D. Skibbe, P. Schnable Lab, Iowa State University)Page 3
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