PALM Applications Focus: Plant Sciences

PALM ApplicationsFocus: Plant SciencesLaser Microdissection & Pressure Catapulting of Giant Cells induced by root Nematodes (aparasitic worm) in infected tomato roots.a) Tissue section (paraffin embedded) of aJ2 nematode (N) surrounded by severalGiant Cells (*).b) Laser microdissection of cytoplasmiccontents of the Giant Cellsc) Actin and MAPK gene expression by RT-PCR from control healthy tissue and GiantCell extracted by LMPC. M: 100-bp laddermarker.1, 4: Positive controls2, 5: LMPC samples indicating the twogenes expressed in Giant Cells3, 6: Negative controls for actin (3) and forMAPK (6)Ramsay et al., 2004Laser Microdissection & Pressure Catapulting of stomata cells from Arabidopsis epidermal peel(Toluidine blue staining)a) b) c)a) Overview before lasermicrodissection. Autocircleoutline of stomata cell;b) Selected area after LMPC,c) Stomata cell in collectionvial ready for analysisPage 4