28409480 AA Äpurifier BR.indd - GE Healthcare Life Sciences

GE Healthcare
Design your own FPLC
purification system
ÄKTApurifier and
automation kits

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Versatility and automation
Automating FPLC protein purification using a chromatography system
ensures more reliable results and greater versatility. Automation eliminates
the problem of sample loss through, for example, spillage or misplaced tubes,
ensuring you get more of the target protein. Furthermore, automation makes
screening of optimal purification conditions much easier. You’ll also save hours
on purification, so you can focus on other important tasks.
Tailor-made solutions for your
purification needs
ÄKTApurifier core systems combined with automation kits allow personalized
system configurations, which reduces or eliminates a range of problems associated
with FPLC purification using a chromatography system—long sample loading time,
frequent column cleaning, and problems caused by air entering the system will
all become things of the past. ÄKTApurifier, together with the new ÄKTAdesign
automations kits and hardware ensures that your purification efforts are always
focused on your target protein(s) of interest. Purification can be performed
at microgram, milligram, or gram scale, depending on the core system and
automation kits you select. All this translates into improved versatility and tailormade
solutions for your target protein(s) of interest, with lower costs as an added
bonus.

Keep costs down
Optional ÄKTAdesign automation kits and hardware make sure you only build a
system for your specific protein purification, which both minimizes purchasing
costs and saves you time. The versatility provided by the automation kits allows
you to add on functionality at a later stage as your needs grow.
Reproducible results
Optimized purification protocols and automation kits translate to consistently high
yield and purity.
Fewer time-consuming steps
Functionalities allowing automated buffer selection, sample application, and media
screening remove tedious, labor-intensive steps, giving you more time to work on
other important tasks.
Prepacked columns increase your chances of
successful purification
Using ÄKTApurifier together with GE Healthcare’s extensive range of prepacked
columns for purification of almost every type of protein not only provides
convenience, but will allow you to resolve more protein.
Intuitive system design
ÄKTApurifier systems ensure that every column is run optimally and is protected
from damaging events such as overpressure. Select a prepacked column from the
extensive column library and ÄKTApurifier automatically sets the optimal running
parameters for that column. New columns can be added to the library and will be
run in the same secure way.
Simplified system control with UNICORN
software
The system’s UNICORN software automatically provides method Wizards for
simple method creation and controls and manages system hardware, saving you
time and giving you better control of each purification run. Results are presented in
a simple-to-follow report format, ready for publication.
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Start with a core system . . .
Advanced pumps ensure reliable results
The main challenges of a chromatography system pump are to achieve accurate
gradient formation over a broad flow rate range and avoid introducing air into the
system. ÄKTApurifier 10 and UPC 10 systems are equipped with Pump P-901, which
enables a wide flow rate range for both laboratory- and pilot-scale applications.
ÄKTApurifier 100 and UPC 100 systems use Pump P-903, which operates at the
lower flow rate and the higher pressure required for running high-resolution
analyses at laboratory scale.
Visualize more protein
ÄKTApurifier 10 and 100 core systems are equipped with Monitor UV-900,
which allows multiple UV-wavelength monitoring of up to three wavelengths
simultaneously. Now you can visualize more protein when adequate detection
cannot be achieved at 280 nm. The novel xenon flash lamp of Monitor UV-900
ensures long lamp lifetime and that no heating of the sample in the flow cell occurs.
ÄKTApurifier UPC 10 and 100 core systems each offer convenient UV-wavelength
monitoring, pH, and conductivity measurement at the same time using Monitor
UPC-900. This monitor gives greater flexibility and provides an economical
alternative to Monitor UV-900.
ÄKTApurifier 10 and 100 core system ÄKTApurifier UPC 10 and 100 core system

. . . and expand
Match an ÄKTApurifier system to
your scale of purification
For purification of proteins at microgram and milligram scale, choose ÄKTApurifier
10 and UPC 10 core systems. Purification of larger, gram-scale quantities of protein
is easily achieved with ÄKTApurifier 100 and UPC 100 systems.
The core configurations of ÄKTApurifier can be easily expanded into advanced
setups to meet your individual purification needs using ÄKTAdesign automation
kits. The range of ÄKTAdesign automation kits enables easier buffer control,
automation, and fractionation.
ÄKTApurifier 10/100 ÄKTApurifier UPC 10/100
10 or 100 ml/min
Pump & Base
Monitor
UV-900
Monitor pH/C-900
(optional)
BufferPrep Kit
ÄKTAdesign
automation kits
Fraction Collectors
Autosamplers
10 or 100 ml/min
Pump & Base
Monitor
UPC-900
ÄKTApurifier core system configurations are advanced chromatography
systems that give you almost unlimited expansion possibilities.
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ÄKTAdesign automation kits
—spend less time at the lab bench
ÄKTApurifier systems eliminate much of the tedious and time-consuming
manual steps involved in sample application by bringing a greater degree of
automation to the system’s setup. Optional ÄKTAdesign automation kits and
hardware are available to add functionality to your ÄKTApurifier core systems
and make sure you get the most from your protein purification.
Examples of additional functionality that can be applied to ÄKTApurifier core systems using
ÄKTAdesign automation kits.
Keep your system and buffers free from air
Air in an expensive gel filtration column can be devastating, ruining the sample run.
Air usually enters the system when the sample is completely applied to a column
and the sample reservoir is empty. Replacing or replenishing depleted stocks
of buffer is time-consuming and makes overnight, unmanned purification runs
difficult. In a worst-case scenario, air in the system resulting from depleted buffer
stocks can lead to irreversible column damage.
Air sensor Kit contains all you need to exclude air from ÄKTApurifier during
purification. Air sensor Kit, which is controlled by UNICORN software, detects air
in the system, and pauses the purification run. The kit therefore prevents column
damage caused by air and secures that the sample is completely injected.
Buffer selection Kit manages buffer reagents effectively, allowing buffer screening
and minimizing the risk of air entering the system. The kit enables automatic
selection among up to eight different buffers to allow optimized purification. Buffer
selection Kit eliminates manual changing of buffers and moving inlet tubing,
ensuring that no air enters the system.

Speed up buffer preparation with BufferPrep
The accuracy of pH is crucial in many separations. BufferPrep Kit, which is optional
for ÄKTApurifier systems, eliminates time-consuming manual buffer preparation
and titration usually needed for pH changes in chromatography. For any pH and
salt concentration entered, BufferPrep automatically calculates the composition of
the buffer online. The accuracy of BufferPrep greatly increases the reproducibility
of results from day to day by eliminating and correcting errors that arise when
mixing buffers manually. BufferPrep enables automatic buffer titration and
eliminates errors occurring during manual preparation.
The BufferPrep function can also be used for pH scouting in rapid method
optimization, all under the control of UNICORN software.
Reliable sample application
Sample application presents a number of significant challenges in protein
purification. Loading samples is time-consuming and managing setups for
different sample volumes can be difficult. Viscous samples provide an additional
risk of system overpressurization and inadequate sample loading can lead to air
inadvertently entering the system.
Increased automation using the optional Sample application Kit can eliminate
many of the problems associated with sample loading. Sample application Kit
gives you the versatility of loading small, medium, or large sample volumes directly
to the column or via capillary or Superloop. When speed is an issue, the pump
supplied in the kit can be modified for automatic, multiple sample injection.
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Convenient media and column screening
Finding the best column for your gel filtration, optimal pH for your ion exchange
purification, or right binding and elution buffer concentration for your histidinetagged
protein purification is laborious, especially when performed manually. Or
perhaps you are unsure as to what column to use?
Column screening Kit provides the necessary motor valves to allow automated
method optimization, multistep purification, and easier column selection. Up to
eight columns can be connected to the system in one go. Column performance can
be easily compared while changing conditions in a run. Preset protocols prevent
system overpressurization and prevent column breakage during use.
Connecting two columns to one of the motor valves included in the kit enables
automated column selection for optimizing both column and media performance,
as well as column switching for two-step purification protocols.
Reversing flow speeds up affinity
chromatography purification
If the full capacity of affinity columns is not used, dilution may occur during
dilution of the target protein, which increases elution time and broadens peaks. To
combat this, elution is preferentially performed in the reverse direction to sample
application.
Reverse flow Kit contains motor valves for automated reverse flow through a
column. Strongly bound target proteins elute more effectively, speeding up your
purification. Resulting peaks are sharper and more concentrated target protein is
recovered. The kit increases the life expectancy of your columns since reversing the
flow efficiently rinses impurity away from the top of the column.
Collect any volume of purified product
Large sample volumes inevitably lead to large elution volumes. If your protein of
interest elutes in a large volume, collecting in small sample tubes becomes timeconsuming
and prone to error.
Fractionation Kit enables collection of large volumes of purified sample. Up to
eight vessels of any size can be used to collect fractions. Collection in large vessels
rather than smaller vessels minimizes the risk of losing valuable target protein,
improving protein recovery and giving you greater piece-of-mind.

Simplify FPLC purification even
further with ÄKTA accessories
In addition to the ÄKTAdesign automation kits, a wide range of
hardware accessories is available to simplify protein purification
with ÄKTApurifier systems.
Flexible fraction collection
Automated fraction collection speeds up fraction collection as well as minimizes
peak dilution and cross-contamination.
Fraction Collector Frac-950 is specifically designed for collection in 96-well
microplates, as well as 12-, 18-, and 30-mm diameter tubes. Fraction Collector
Frac-920 allows collection of up to 175 fractions in 12-mm diameter tubes, up to 95
fractions in 10–18 mm tubes, as well as 40 fractions in 30-mm diameter tubes. Both
fraction collectors are available as options for ÄKTApurifier core systems.
Monitor pH/C-900
Monitor pH/C-900 expands ÄKTApurifier 10 and 100 systems to allow measurement
of conductivity, pH, and temperature. Since temperature can influence both pH
and conductivity, the monitor compensates for temperature variations of these
readings. Self-diagnostics ensure continual reliability and reproducibility. Both pH
and conductivity/temperature flow cells feature low cell volume and high linearity
for accurate measurements.
Faster sample injection using autosamplers
Autosamplers give you more time to work on other projects during the day, and
increase throughput by enabling overnight purification runs.
Autosampler A-900 is available with Peltier cooling, which effectively protects
heat-labile proteins during sampling. The autosampler is used with 7 or 12 mm
outer diameter vials. Autosampler A-905 is designed for sample injection from
microplates, as well as microvials, with Peltier cooling as standard.
Add additional instruments easily
AD-900 Converter is an analog/digital converter for connecting external
instruments to UNICORN control software. The AD-900 Converter allows easier
monitoring of signals received as voltage inputs from external sources such as
fluorescence detectors, radioactivity monitors, or diode arrays.
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Rest easy—let UNICORN
manage the system
Select technique
Select column
Design your own
ÄKTApurifier online
Bringing control software into the Windows environment
and of course onto a PC means things get a lot simpler.
Designed for Windows 2000 and Windows XP, UNICORN
Control System software gives you real-time control of
protein purification systems. UNICORN control software
gives the user an overview of the system and actual system
status, while the method Wizard allows simple control of your
purification. The software provides an interface that allows
the user to better manage and understand the hardware, its
operation and configuration.
UNICORN control software:
• Makes every step easy
• Provides simple interface and method wizard
• Automatically evaluates and presents results
• Scouts for optimal purification conditions overnight
• Shares data on a corporate network
• Compliant with 21 CFR part 11
Now you can configure an ÄKTApurifier system directly
on the on the World Wide Web using the new ÄKTApurifier
configurator tool at www.gehealthcare.com/akta. This
new, intuitive function guides you through ÄKTApurifier core
systems and available options with the help of multiplechoice
questions, allowing you to find exactly the right
system for your research needs. The tool allows you to select
a system and appropriate ÄKTA automation kits without
slowing you down with in-depth technical specifications.

Ordering information
Product Code No.
ÄKTApurifier 10 28-4062-64
ÄKTApurifier 100 28-4062-66
ÄKTApurifier UPC 10 28-4062-68
ÄKTApurifier UPC 100 28-4062-71
Monitor pH/C-900 18-1107-76
Increased automation: automatic column, media, or buffer scouting
ÄKTAextension equipment holder 18-1158-31
Buffer selection Kit
(Includes Valve IV-908, 1 UniNet cable) 11-0035-96
Column screening Kit
(Includes 2 valves PV-908, 1 UniNet cable) 11-0035-95
Sample application Kit
(includes Pump P-960, Sample valve Kit) 11-0036-00
BufferPrep Kit
(includes valve SV-903 and tubing) 28-4044-73
Air sensor Kit
(includes Air-900 control box, purge valve,
and air sensor Air-925 N) 11-0035-98
Reverse flow Kit
(includes valve INV-907, tubing, and connectors) 11-0035-97
Sample application
Superloop 10 ml (ÄKTAdesign), load 1–10 ml 18-1113-81
Superloop 50 ml (ÄKTAdesign), load 1–50 ml 18-1113-82
Superloop 150 ml, load 1–150 ml
(requires Union 1/16” female/M6 male fitting)
18-1023-85
Union 1/16” female/M6 male fitting 18-1112-57
Fraction collection
Fraction Collector Frac-950 18-6083-00
Fraction Collector Frac-920 18-1177-40
Autosamplers
Autosampler A-900 18-1116-61
Autosampler A-900 with cooling 18-1144-61
Autosampler A-905 (for ÄKTAdesign systems) 18-1175-93
Additional items
UNICORN analysis module 18-1134-74
AD-900 Converter
(includes UniNet cable 0.7 m, Mini-DIN cable 1.5 m) 18-1148-62
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www.gehealthcare.com/akta
www.gehealthcare.com
GE Healthcare Bio-Sciences AB
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751 84 Uppsala
Sweden
ÄKTAdesign, ÄKTApurifier, Drop design, FPLC, Superloop, and UNICORN are trademarks of
GE Healthcare companies. GE, imagination at work, and GE monogram are trademarks of
General Electric Company.
© 2006 General Electric Company – All rights reserved.
GE Healthcare Bio-Sciences AB, a General Electric Company.
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28-4094-80 AA 05/2006