September 2007 - Wadsworth Center

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Sequences alignment:Query 1 TCCGTAGGTGAACCTGCGGAAGGATCATTACTGATTTGCTTAATTGCACCACATGTGTTT 60||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||AY139791 1 TCCGTAGGTGAACCTGCGGAAGGATCATTACTGATTTGCTTAATTGCACCACATGTGTTT 60Query 61 TTTACTGGACAGCTGCTTTGGCGGTGGGGACTCGTTTCCGCCGCCAGAGGTCACAACTAA 120||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||AY139791 61 TTTACTGGACAGCTGCTTTGGCGGTGGGGACTCGTTTCCGCCGCCAGAGGTCACAACTAA 120Query 121 ACCAAACTTTTTATTACCAGTCAACCATACGTTTTAATAGTCAAAACTTTCAACAACGGA 180||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||AY139791 121 ACCAAACTTTTTATTACCAGTCAACCATACGTTTTAATAGTCAAAACTTTCAACAACGGA 180Query 181 TCTCTTGGTTCTCGCATCGATGAAGAACGCAGC 213|||||||||||||||||||||||||||||||||AY139791 181 TCTCTTGGTTCTCGCATCGATGAAGAACGCAGC 213Alignment of primary sequence of the ITS1 regions of C. viswanathii ATCC 22981 and PT specimen C. viswanathii NYSDOH0907.Query 1 GATATTCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCAAAGGGC 60||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||DQ249200 1 GATATTCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCAAAGGGC 60Query 61 ATGCCTGTTTGAGCGTCATTTCTCCCTCAAGCCCGCGGGTTTGGTGTTGAGCAATACGCC 120||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||DQ249200 61 ATGCCTGTTTGAGCGTCATTTCTCCCTCAAGCCCGCGGGTTTGGTGTTGAGCAATACGCC 120Query 121 AGGTTTGTTTGAAAGACGTACGTGGAGACTATATTAGCGACTTAGGTTCTACCAAAACGC 180||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||DQ249200 121 AGGTTTGTTTGAAAGACGTACGTGGAGACTATATTAGCGACTTAGGTTCTACCAAAACGC 180Query 181 TTGTGCAGTCGGCCCACCACAGCTTTTCTAACTTTTGACCTCAAATCAGGTAGGACTACC 240||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||DQ249200 181 TTGTGCAGTCGGCCCACCACAGCTTTTCTAACTTTTGACCTCAAATCAGGTAGGACTACC 240Query 241 CGCTGAACTTAAGCATATCAATAAGCGGAGGAAAA 275|||||||||||||||||||||||||||||||||||DQ249200 241 CGCTGAACTTAAGCATATCAATAAGCGGAGGAAAA 275Alignment of primary sequence of the ITS2 regions of C. viswanathii WM 239 and PT specimen C. viswanathii NYSDOH 0907.Further Reading:1. Lee, F.L., Fu, H.M., and Hsu, W.H. 1998. DNA hybridization and electrokaryotype study of someCandida species. Int. J. Syst. Bacteriol. 48: 1463-1466.2. Maiwald, M., Kappe, R., and Sonntag, H.G. 1994. Rapid presumptive identification of medically relevantyeasts to the species level by polymerase chain reaction and restriction enzyme analysis. J. Med. Vet.Mycol. 32: 115-122.3. McGinnis, M.R. 1983. Detection of fungi in cerebrospinal fluid. Am. J. Med. 75(1B):129-138.4. Quindos, G., Lipperheide, V., and Ponton, J. 1993. Evaluation of two commercialized systems for therapid identification of medically important yeasts. Mycoses 36: 299-303.5. Sandhu, D.K., Sandhu, R.S., and Misra, V.C. 1976. Isolation of Candida viswanathii from cerebrospinalfluid. Sabouraudia14: 251-254.40
Figure 17. Four-day-old, white, a little wrinkled, and moist colony of Candida viswanathii on Sabouraud’sdextrose agar.A. B.Figure 18. Microscopic morphology of Candida viswanathii on corn meal agar with Tween 80 showing longpseudohyphae and oval shaped blastoconidia (A, 400 X magnification; B, line diagram not to scale).41
Page 3 and 4: CONTENTSContents 3Schedules 4Test S
Page 6 and 7: A failure to attain an overall scor
Page 8 and 9: LABORATORY PERFORMANCE SUMMARYMycol
Page 10 and 11: MOLD DESCRIPTIONSM-1 Malbranchea sp
Page 12 and 13: 3. Kaufman, L., Standard, P.G., Hup
Page 14 and 15: A. B.Figure 1. (A) One-week-old col
Page 16 and 17: Query 121 GTCCGCCGGGGAAAACCAAACCCTG
Page 18 and 19: A. B.Figure 3. (A) Seven-day-old, p
Page 20 and 21: ectangular, of the same diameter as
Page 22 and 23: TABLE 2: Differentiation of Chrysos
Page 24 and 25: M-4 Fusarium sp.Source: NailsScorin
Page 26 and 27: A. B.Figure 7. (L) Seven-day-old, w
Page 28 and 29: A. B.Figure 9. (A) Seven-day-old, w
Page 30 and 31: Query 180 TTGGTTCTGGCATCGATGAAGAACG
Page 32 and 33: YEAST DESCRIPTIONSY-1 Candida dubli
Page 34 and 35: Alignment of primary sequence of th
Page 36 and 37: Y-2 Rhodotorula minutaSource: Cathe
Page 38 and 39: Figure 15. Seven-day-old, soft, smo
Page 42 and 43: Y-4 Candida parapsilosisSource: Sto
Page 44 and 45: 8. Yalaz, M., Akisu, M., Hilmioglu,
Page 46 and 47: Y-5 Trichosporon asahiiSource: Bloo
Page 48 and 49: 5. Panagopoulou, P., Evdoridou, J.,
Page 50 and 51: ANTIFUNGAL SUSCEPTIBILITY TESTINGIn
Page 52 and 53: DIRECT DETECTION (CRYPTOCOCCUS NEOF
Page 54 and 55: Table 4. Summary of quantitative as
Page 56 and 57: 25. Rebell, G. and Taplin, D. 1972.

September 2007 - Wadsworth Center

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