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Figure 5.25: Transverse sections of the embryo of Prumnopitys taxifolia showthe arrangement of the developing vascular tissues in relation tothe cotyledons.A - H : A mature embryo within the female gametophyte (fg), in a non-adj<strong>ac</strong>entseries of TS. The gametophyte centreline has been drawn on e<strong>ac</strong>h image to showthat this particular embryo has diagonal orientation with respect to the broad axis (ba)of the gametophyte. Scale bar (A) = 500 pm. Scale bars (B-H) = 100 pm.A : Whole female gametophyte (fg) with the embryo (e), sectioned at the level ofthe upper hypocotyl (see also E). The dark line around the perimeter is allthatremains of the parietal layers (pl) of the nucellus, reduced to a papery'skin'. Notethat the difference between the broad and narrow axes of the gametophyte has beenexaggerated by sectioning pressure. The gametophyte was also damaged duringremoval of the hard seed coat.B : The crushed suspensor (s) lies in the cavity toward the micropylar end of thegametophyte.C : Dead cells are present at the tip of the root cap (rt).D : ln the lower hypocotyl, the developing vascular tissues are radially symmetrical.Recognisable tissues are: epidermis (ep), isolated tanniniferous cells (tc) under theepidermis, cortex (cx), six secretory canals (arrowheads), and pith (pi) at the centre ofthe procambialtissue.E : Detail of the same section as shown in A. In the upper hypocotyl, the vasculartissue arrangement becomes a rectangle defined by the six secretory canals(arrowheads) and two bands of procambial tissues (pc), with pith at the centre.F : Just below the cotyledons, only the four outer secretory canals (arrowheads) arepresent, with four zones of procambialtissue (pc).G : At the base of the cotyledons (cot), the shoot apex (sa) extends the full width ofthe embryo, with zones of meristematic tissue (arrows) at both ends. E<strong>ac</strong>h cotyledonhas two vascular bundles (vb), e<strong>ac</strong>h <strong>ac</strong>companied by a secretory canal.H : The cells at the tip of the shoot apex (sa) are quiescent. At this level, the twovascular bundles are more clearly defined in e<strong>ac</strong>h cotyledon (cot). The adaxialf<strong>ac</strong>esof the cotyledons meet above the shoot apex.

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