2012 Program (PDF) - International Society of Chemical Ecology

Monday 23rd July, 16:45 – 17:00 hChemical analysis of methyl jasmonate treated Scots pine Pinus sylvestrisusing GC-MS and LC-MSLUNDBORG L 1 , MOREIRA X 2 , ZAS R 3 , SAMPEDRO L 4 , BJÖRKLUND N 5 , HELLQVIST C 5 ,NORDLANDER G 5 , AND BORG-KARLSON A-K 1,61Royal Institute of Technology, Department of Chemistry, Ecological Chemistry group,Stockholm, SWEDEN2Department of Ecology and Evolutionary Biology, University of California, 92697 Irvine,California , USA3Misión Biológica de Galicia, Consejo Superior de Investigaciones Científicas, Pontevedra,SPAIN4Centro de Investigación – Forestal de Lourizán – Unidad Asociada MBG-CSIC, Pontevedra,SPAIN5Swedish University of Agricultural Sciences, Department of Ecology, Uppsala, SWEDEN6 Tartu University, Institute of Technology Tartu, ESTONIAlinalun@kth.seIn my PhD work I analyze conifer constituents that seedlings produce and emit into theenvironment. The aim is to identify chemical resistance markers in Scots pine Pinussylvestris against the large pine weevil Hylobius abietis. This insect is one of theeconomically most important forest pests in Sweden and almost 90% of the seedlings coulddie without protective measures such as shelterwood and soil scarification (1). Insecticidesadd to the protection of the seedlings but the future prohibition call for replacement. Thesignal hormone methyl jasmonate is exogenously applied to young conifers in order toinduce chemical defence by mimicking insect herbivory (2,3). Based on biological resultsfrom field trials in Sweden and in Spain, I evaluate stress responses from seedlings usingGC-MS and LC-MS. From this I have found induction of β-pinene in Scots pine, afterpretreatment with methyl jasmonate, and in Maritime pine Pinus pinaster and Monterey pinePinus radiata after pine weevil infestation. The induction is observed in both stem andneedles. Further I investigate possible activation of the (-)-pinene-synthase using chiralanalysis with 2D-GC-MS.References1. Peterson M and Örlander G. Canadian Journal of Forest Research (2003) 33(1):64–732. Moreira X et al. Plant Defence: Biological Control, Vol. 12 (2012):345–3623. Moreira X et al. Quantitative comparison of chemical, biological and mechanical inductionof secondary compounds in Pinus pinaster seedlings (2012). Trees, Structure and Function26:677-683AcknowledgementsThe research is supported by the Swedish Foundation of Strategic Research.44