APPENDIX IINVESTIGATION PROCEDURESThe <strong>in</strong>vestigation <strong>of</strong> suspected pesticide poison<strong>in</strong>g <strong>in</strong>cidents relies on a scheme, which allowsmembers <strong>of</strong> the public and <strong>in</strong>terested organisations to submit carcases, suspected baits orother samples for pesticide analysis. The Wildlife Incident Investigation Scheme is operated<strong>in</strong> Scotland by the Chemistry Branch at <strong>SASA</strong> on behalf <strong>of</strong> the Scottish Government.Agricultural Staff <strong>in</strong> area <strong>of</strong>fices located throughout Scotland, provide support whennecessary for field <strong>in</strong>vestigations, and also act as an additional po<strong>in</strong>t <strong>of</strong> contact fornotification <strong>of</strong> <strong>in</strong>cidents.A number <strong>of</strong> environmental and animal welfare organisations, such as the RSPB-Scotlandand the Scottish Society for the Prevention <strong>of</strong> Cruelty to <strong>Animals</strong>, play an active role <strong>in</strong> some<strong>in</strong>cident <strong>in</strong>vestigations. These bodies act not only by assist<strong>in</strong>g members <strong>of</strong> the public tonotify <strong>in</strong>cidents, but also by screen<strong>in</strong>g out <strong>in</strong>appropriate cases prior to notification.The Scottish Agricultural College (SAC) Veter<strong>in</strong>ary Service acts <strong>in</strong> partnership with thescheme, <strong>in</strong> forward<strong>in</strong>g relevant samples to <strong>SASA</strong> from potential <strong>in</strong>cidents notified <strong>in</strong>directlyvia its laboratories, and by screen<strong>in</strong>g out <strong>in</strong>cidents that are unlikely to <strong>in</strong>volve pesticides. TheLasswade Veter<strong>in</strong>ary Laboratory is used to provide specialist pathological support to <strong>SASA</strong>on wild animals and also provides an additional route <strong>in</strong>to the scheme. The post mortemexam<strong>in</strong>ations undertaken by these laboratories may identify disease, trauma, starvation orother causes <strong>of</strong> death, elim<strong>in</strong>at<strong>in</strong>g the need for expensive analytical <strong>in</strong>vestigation.As well as <strong>in</strong>vestigat<strong>in</strong>g <strong>in</strong>cidents <strong>in</strong>volv<strong>in</strong>g wildlife, the scheme covers suspected poison<strong>in</strong>g<strong>of</strong> livestock, companion animals, and honeybees, suspicious materials and substances andsuspected baits. Incidents may be rejected if they fall out with the remit <strong>of</strong> the scheme, or ifother acceptance criteria are not met.<strong>SASA</strong> makes use <strong>of</strong> various analytical techniques, methods and <strong>in</strong>strumentation to identifyand quantify s<strong>in</strong>gle or multiple pesticide-residues. Two different multi-pesticide residuemethods are currently used to determ<strong>in</strong>e and quantify:(1) Organochlor<strong>in</strong>e, organophosphorus, carbamate and pyrethroid compounds(2) Anticoagulant rodenticides.These multi-residue methods are supplemented by compound-specific (i.e. s<strong>in</strong>gle-residue)analytical methods developed for the determ<strong>in</strong>ation <strong>of</strong> chloralose, cyanide, metaldehyde,paraquat, phosph<strong>in</strong>e and strychn<strong>in</strong>e. Wherever possible, residues are confirmed us<strong>in</strong>g analternative analytical technique or measurement parameter.Field <strong>in</strong>vestigations are normally only triggered follow<strong>in</strong>g the positive detection andidentification <strong>of</strong> pesticide(s) <strong>in</strong> or on the test specimen. However, field <strong>in</strong>vestigations mayalso be <strong>in</strong>itiated if sufficient evidence <strong>of</strong> pesticide <strong>in</strong>volvement is available e.g. follow<strong>in</strong>gdirect notification or after post-mortem exam<strong>in</strong>ation.Analytical results, post-mortem f<strong>in</strong>d<strong>in</strong>gs and field <strong>in</strong>vestigation reports are collated and<strong>in</strong>terpreted by <strong>SASA</strong> Chemistry Branch <strong>in</strong> order to categorise an <strong>in</strong>cident and to determ<strong>in</strong>e11
whether residue levels detected contributed to the death or illness <strong>of</strong> the animal <strong>in</strong>volved. Insome cases, the presence <strong>of</strong> residues <strong>in</strong> association with typical post-mortem f<strong>in</strong>d<strong>in</strong>gs may beused to determ<strong>in</strong>e mortality.The results <strong>of</strong> <strong>in</strong>vestigations <strong>in</strong> Scotland are presented annually <strong>in</strong> this report series and formpart <strong>of</strong> the Advisory Committee on <strong>Pesticide</strong>s - Environmental Panel report series publishedby Defra. The regulatory body, The Chemicals Regulation Directorate, is able to assessrelevant <strong>in</strong>cident <strong>in</strong>formation for any implications for the approval status <strong>of</strong> a particularpesticide or family <strong>of</strong> pesticides. Where legal proceed<strong>in</strong>gs are used as part <strong>of</strong> enforcementaction, the evidence gathered by <strong>SASA</strong> Chemistry Branch and SG Agricultural Staff, ispresented <strong>in</strong> reports to the Procurator Fiscal Service. Police forces are active partners <strong>in</strong>counter<strong>in</strong>g pesticide abuse and frequently take the lead <strong>in</strong> <strong>in</strong>vestigations and presentation <strong>of</strong>such cases to the Procurator Fiscal.12