Adiponectin

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Effect of ET-1 on adiponectin secretion serum free-treated adipocyte preincubated 1 h Inhibitor / Receptor blocker Inhibitor / Receptor blocker wash 3 times incubated with 10 -8 M ET-1 4 h (presence/absence these inhibitor or receptor blocker) determined adiponectin secretion 14
<strong>Adiponectin</strong> mRNA extraction Tri Reagent® kit 1% agarose gel electrophoresis UV light absorbance at 260 nm 30 min 37°c incubated with RNase-free DNase I inactivated DNase 10 min 100°c 15
Page 2 and 3: Adiponectin ‣ ADIPOQ gene product
Page 4 and 5: Adiponectin actively influences met
Page 6 and 7: Endothelin-1 6
Page 8 and 9: Endothelin-1 ‣ Regulation of meta
Page 10 and 11: Materials and Methods 10
Page 12 and 13: Differentiation procedure Cell were
Page 16 and 17: RT-PCR RNA template Heated, 5 min,
Page 18 and 19: Statistical analysis ‣ mean ± SD
Page 20 and 21: Time-Effect of ET-1 on adiponectin
Page 22 and 23: ET-1 acts through ET A R to decreas
Page 24 and 25: Role of ERK activation on ET-1-down
Page 26 and 27: Effect of ET-1 on adiponectin secre
Page 28 and 29: Acute ET-1 treatment stimulates adi
Page 30 and 31: ERK activation was not involved in
Page 32 and 33: 2-APB ET-1 ET-1 2-APB ET-1 signific
Page 34 and 35: Major finding of this study ET-1 ha
Page 36 and 37: Adiponectin secretion Short-term 2-
Page 38 and 39: Bedi et al. ET-1 did not regulate a
Page 40 and 41: Bedi et al. Chronic ET-1 treatment
Page 42 and 43: Lee YC et al, ET-1 ET A R impairs i
Page 44 and 45: Adiponectin insulin-sensitizing adi
Page 46 and 47: Adipocyte-derived adiponectin • p
Page 48 and 49: ET-1 adipocyte differentiation (H
Page 50 and 51: Dysregulation of the action of ET-1
Page 52 and 53: Bost et al. essential and specific
Page 54 and 55: Conclusion ET-1 has distinct regula
Page 56 and 57: Conclusion(cont.) Cardiovascular sy

Adiponectin

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