Pleurotus ostreatus invaluable source of laccases for industrial production
A.101 - SANNIA Giovanni
A.101 - SANNIA Giovanni
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P. <strong>ostreatus</strong> laccase functional evolution<br />
Mutant Generation activity<br />
pH stability<br />
pH3 pH5 pH7 pH10<br />
Mutant Generation activity<br />
T<br />
stability<br />
1M9B I 1.5x Χ Χ Χ Χ Χ<br />
pH stability<br />
pH3 pH5 pH7 pH10<br />
T stability<br />
1L2B II 2.5x Χ √ Χ √ √<br />
1M10B II 2.5x Χ √ √ √ √<br />
3M7C II 2.5x Χ √ √ √ √<br />
Three generation <strong>of</strong> libraries<br />
(3,300 variants) have been<br />
screened using different<br />
criteria, and seven variants<br />
endowed with improved<br />
features have been selected<br />
Mutant Generation activity<br />
pH stability<br />
pH3 pH5 pH7 pH10<br />
T<br />
stability<br />
R4 R4 2.5x Χ √ √ √ √<br />
Mutant Generation activity<br />
pH stability<br />
pH3 pH5 pH7 pH10<br />
T<br />
stability<br />
1H6C III 4.5x Χ √ √ √ √<br />
4M10G III 4.5x Χ √ √ √ √<br />
Functional evolution<br />
Festa G., et al., Proteins 2008<br />
Miele A., et al., Enz. Microb. Technol. 2009<br />
Miele A., et al., Mol Biotechnol. 2010<br />
IPBT 2011, Milan Giovanni Sannia
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