Pleurotus ostreatus invaluable source of laccases for industrial production
A.101 - SANNIA Giovanni
A.101 - SANNIA Giovanni
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P. <strong>ostreatus</strong> laccase functional evolution<br />
Specific<br />
Activity<br />
(U/mg)<br />
Stability pH<br />
t ½ (days)<br />
Stability T<br />
t ½ (h)<br />
pH3 pH5 pH7 pH10 pH7, 60°C<br />
Wild type 155 ± 1 5.8 10.3 11.3 30.0 4.5<br />
1H6C 700 ± 1 3 35 39 63 7.2<br />
The best selected mutant, 1H6C, and the wild-type enzyme, POXA1b, were<br />
heterologously expressed in Aspergillus niger<br />
Aspergillus niger<br />
POXA1b<br />
1H6C<br />
Extracellular Activity 38,500 U L -1 55,500 U L -1<br />
IPBT 2011, Milan Giovanni Sannia
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