Microbial Bioinsecticide
Bacillus thuringiensis Bacillus thuringiensis
Bacillus thuringiensis Bacillus thuringiensis
- No tags were found...
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
B. thuringiensis subsp kurstakii<br />
• Protoxin gene on one of 7 different plasmid: 2.0,<br />
7.4, 7.8, 8.2, 14.4, 45, and 71 kb. Which plasmid<br />
carries protoxin gene?......... By Succrose Grdient<br />
• Three fractions: small (2 kb); medium (7.4, 7.8,<br />
8.2, 14.4 kb); large (45, and 71 kb).<br />
• Medium and large are partially digested with<br />
Sau3AI and ligated into BamHI site of plasmid<br />
pBR322.<br />
• Transformation to E. coli, and screening by:
Change language