INCIDENCE OF VIRUS INFECTIONS ON DIFFERENT PEACH CULTIVARS IN MONTENEGRO

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• Sequence analyses of CP gene from PNRSV isolates proved to be 89.3 – 100% identical with corresponding sequences of isolates previously described • 2 PNRSV isolates from cv. Rita Star were most closely related to Chilean NctCl.augl isolate from nectarine (EF565253) • PNRSV isolates from cv.s May Crest and Spring Crest were most closely related to Italian isolate PchIt.may1 from peach cv. May Crest
• Sequences of 4 PNRSV isolates were deposited in GenBank (JX569825 - JX569828) • Isolates clustered within three groups: PE-5, PV-32 and PV- 96 • 2 MNE isolates from cv. Rita Star clustered in PE-5 group • Isolates from cv. May Crest and Spring Crest clustered in PV-96 Phylogenetic tree was constructed using minimum-evolution method with Tamura-Nei corrected nt distance and bootstrap analyses of 10000 replicates within MEGA 4.1 software
Page 1 and 2: INCIDENCE OF VIRUS INFECTIONS ON DI
Page 3 and 4: Investigation on peach viruses Prev
Page 5 and 6: Examined cultivars and viruses Cul
Page 7 and 8: Field survey cv. Ritastar cv. Ritas
Page 10 and 11: Molecular methods used for the dete
Page 12 and 13: Incidence of PPV, PNRSV and PDV in
Page 14 and 15: Purification of PCR products Clonin
Page 16 and 17: Phylogenetic analysis • Phylogene
Page 18 and 19: Molecular analysis for PNRSV • De
Page 22 and 23: Molecular analysis for PDV • RT-P
Page 24 and 25: • Real Time RT-PCR identified mor
Page 26: Thank you for your attention!!!

INCIDENCE OF VIRUS INFECTIONS ON DIFFERENT PEACH CULTIVARS IN MONTENEGRO

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