INCIDENCE OF VIRUS INFECTIONS ON DIFFERENT PEACH CULTIVARS IN MONTENEGRO

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• Real Time RT-PCR identified more PPV infected samples than RT-PCR indicating higher sensitivity • Amplification of different amplicons from the detected viruses by RT-PCR, followed by cloning of complete and partial sequences of CP gene allowed phylogenetic analysis of isolates of different detected viruses: PPV (8), PNRSV (4) and PDV (1) • Sequence analyses revealed that all Montenegrin PPV isolates shared from 92.2 to 99.1% nucleotide identity with corresponding PPV-M strain sequences deposited in GenBank
• Results indicate the most probable introduction of PPV in Montenegro through peach propagation material which is mainly imported from Greece where this strain is the most prevailing one • Phylogenetic analysis suggesting two possible introductions of PNRSV in Montenegro • Molecular grouping of PNRSV isolates wasn’t in correlation with geographical region and host • Urgent sanitation measures should be taken: eradication of infected trees severe control related to importation of plant material
Page 1 and 2: INCIDENCE OF VIRUS INFECTIONS ON DI
Page 3 and 4: Investigation on peach viruses Prev
Page 5 and 6: Examined cultivars and viruses Cul
Page 7 and 8: Field survey cv. Ritastar cv. Ritas
Page 10 and 11: Molecular methods used for the dete
Page 12 and 13: Incidence of PPV, PNRSV and PDV in
Page 14 and 15: Purification of PCR products Clonin
Page 16 and 17: Phylogenetic analysis • Phylogene
Page 18 and 19: Molecular analysis for PNRSV • De
Page 20 and 21: • Sequence analyses of CP gene fr
Page 22 and 23: Molecular analysis for PDV • RT-P
Page 26: Thank you for your attention!!!

INCIDENCE OF VIRUS INFECTIONS ON DIFFERENT PEACH CULTIVARS IN MONTENEGRO

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