koff - LEPA

provide information concerning the biological activity of the sample. ELISAs are not generally
used to discriminate active or non-active forms of a protein. It may also detect degraded proteins
that have intact epitopes”.
8. Protein array (from Arrait.com)
“Antibody, Antigen, Peptide and other Protein Microarrays Custom Made to Order. Antibody
microarrays to Cancer, Apoptosis, Growth Factors, Cytokines, Receptors and More... Users can
to assay thousands of specific proteins with the ArrayIt Protein Microarrays. A wide variety of
proteins representing a range of biological functions are detectable. ArrayIt microarrays allow
users to measure differences in protein abundance using a two-color fluorescent labeling
technique that can be provided with the microarrays. “Sandwich” detection schemes can also be
implemented, please discuss your application goals with us. Detection is typically fluorescentbased
using microarray scanners, but microarrays for other detection platforms can be
provided. The protocols are simple, and the detection limits are extremely high.
Figure 15. Protein array scheme
http://www.arrayit.com/Products/Microarrays/
Figure 15. Two color antibody microarray. Compare two biological samples to measure the absolute and relative
differences in protein expression. This procedure is fluorescence-based and compatible with all current microarray
scanners. Extracted proteins are directly labelled (kit provided) and analysed. The covalently immobilized
antibodies capture fluorescently labelled antigens during the reaction step. Normal and cancerous cells can be
compared. The raw data provide a measure of proteins from samples A & B.
22