Supplement

‘‘RAD’’) or INK128 (mTORC1/2 inhibitor, ‘‘INK’’) by daily oral gavage
(I.G.) for 4 weeks with or without 75mg/kg CY I.P. weekly. 8 weeks
following the final treatment, mice were harem-bred with proven male
breeders. Mice were given up to 8 weeks to breed and give birth. Infertility
was defined as no sign of pregnancy by 8 weeks in a harem-breeding cage
with a fertile male partner. The following data were recorded: number of
pups, percent of living pups, weight, and days from male interaction to birth.
Data analysis was performed using student’s t-test (n¼5 mice/group, mean
SEM, Prism 6.0f). A power calculation determined that four female mice per
treatment group would provide 80% power to detect a 50% difference in litter
size with an alpha of 0.05.
RESULTS: 40% of CY-treated mice were infertile, and CY-treated mice
had significantly fewer pups per litter compared to control (3.4 1.7
[n¼17 pups] vs. 8.8 0.5 [n¼44 pups] respectively, P