FINAL PROGRAM AND ABSTRACTS OF THE 17th ESPCR

Program for the 17 th Meeting of the European Society for Pigment Cell Research
Geneva, Switzerland,
Tuesday 11 th to Thursday 13 th of September 2012.
Auditorium B400
Centre Médical Universitaire
1, Rue Michel-Servet
1211 Geneva 4
The Melanocyte and its Environment

Sponsors:
-Krebsliga Schweiz, Ligue Suisse contre le Cancer, Lega Svizzera contro il Cancro
-Krebsfoschung Schweiz, Recherche Suisse contre le Cancer, Ricerca Svizzera contro
il Cancro
-Colorix SA
-Zeiss AG
-Bucher Biotec AG
-Ligue Genevoise contre le Cancer
-Leica Microsystems
-University of Geneva, Switzerland
-European Society for Pigment Cell Research
Local organizing committee:
Nicole Aebischer (Geneva)
Friedrich Beermann (Lausanne)
Lionel Fontao (Geneva)
Marie-Claude Jacquier (Geneva)
Birgit Kastberger (Geneva)
Severine Tabone-Eglinger (Geneva)
Patricia Vazquez (Geneva)
Monique Wehrle-Haller (Geneva)
Bernhard Wehrle-Haller (Geneva)
International experts:
Friedrich Beermann (CH)
Dot Bennett (UK)
Anja Bosserhoff (GER)
Heather Etchevers (F)
Lionel Larue (F)
Marie-Dominique Galibert (F)
Jose-Carlos Garcia-Borron (E)
Robert Kelsh (UK)
Luisa Lanfrancone (I)
Lluis Montoliu (E)
Alessandra Napolitano (I)
Elisabeth Patton (UK)
Mauro Picardo (I)
Lukas Sommer (CH)
Eirikur Steingrimsson (Iceland)
Alain Taïeb (F)
Bernhard Wehrle-Haller (CH)
FAL = Fritz Anders Lecture, KL = keynote lecture,
IS = invited speaker, OP = oral presentation

Program, sessions and speakers (Lecture Hall B400):
8:00-9:30 ESPCR council meeting
1 st day (Tuesday, 11. September 2012)
9:00 Opening of Registration and Poster set up (foyer)
10:00-12:00 Special interest group meeting supported by ESPCR
-Development (B400) -vitiligo (S1-2)
12:30-13:00 Opening ceremony of the 17 th ESPCR meeting:
-Lionel Larue, President of the ESPCR
-Bernhard Wehrle-Haller, Head of the local organizing committee
13:00 – 13:30 Fritz Anders Lecture:
Chair: Lionel Larue
FAL Friedrich Beermann (Lausanne, Switzerland)
Tyrosinase - transgenic mice and beyond ...
13:30 – 15:15 1 st Session: Development of the Melanocyte:
Chairs: Elizabeth Patton and Friedrich Beermann
IS1 Robert Kelsh (Bath, UK)
Expanding our understanding of the zebrafish melanocyte gene regulatory network
IS2 Tosso Leeb (Bern, Switzerland)
Hypopigmentation phenotypes in domestic animals
OP1 Mitch Levesque (Zürich, Switzerland)
Tissue-specific transcriptomes and extracellular protein interactions identify gene
regulatory networks involved in neural crest development and zebrafish pigmentation
OP2 Verena Kottler (Tübingen, Germany)
Kita and csf1ra mutants provide novel insights into pigment pattern formation in
the guppy, Poecilia reticulata
OP3 Davide Seruggia (Madrid, Spain)
The nuclear structure of the mouse tyrosinase locus
OP4 Edouard Reyes-Gomez et al (Maisons-Alfort, France)
Sbno2 overexpression in Dct-expressing cells alters melanocyte and nerve
development in mice
15:15-15:45 Coffee break
15:45 – 16:45 2 nd Session: The Bright and Dark side of the Sun
Chairs: Mauro Picardo and Alain Taïeb
IS3 Alessandra Napolitano (Naples, Italy)
The bright and dark side of red hair pigments
IS4 Marie-Dominique Galibert (Rennes, France)
How does USF1 orchestrate the UV-response?

16:45-18:00 Flash-Talks:
Chair: Lluis Montoliu
OP5 Toyoko Akiyama (Nagoya, Japan)
Effects of pigmentation related genes on the fibromelanosis phenotype in
Silky chickens
OP6 Melanie Domingues (Paris, France)
Increased motility and invasiveness of melanoma cells overexpressing ICAT,
an inhibitor of β-catenin
OP7 Sara Maia (Lisboa, Portugal)
Optimization of a Retinal Pigment Epithelium differentiation protocol driven
by ectopic expression of eye transcription factors
OP8 Lionel Larribère (Heidelberg, Germany)
Human iPS cell-based modeling of hyperpigmentation in Neurofibromatosis
type 1 (NF1)
OP9 Chandana Basu Mallick (Tartu, Estonia)
An insight on SLC24A5 gene and its role in human skin color variation
OP10 Sabine Druillennec (Paris, France)
Raf signaling in melanocyte and melanoma development
OP11 Kate Brown (Norfolk, UK)
Investigating heterogeneous cell populations in melanoma tumours
OP12 Phil Cheng (Zürich, Switzerland)
DNA methylation in melanoma phenotype switching
OP13 Mohammad Krayem (Brussels, Belgium)
Vemurafenib stimulates pigmentation in V600E BRAF melanoma cell lines through
MITF stimulation and cell differentiation
18:00-20:00 Poster Viewing and Welcome Reception (Foyer)
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2 nd day (Wednesday, 12. September 2012)
9:00-10:30 3 rd Session: Adhesion and Migration of Melanocyte and
Keratinocytes
Chairs: Lionel Fontao and Lukas Sommer
IS5 Laura Machesky (Glasgow, UK)
Role of the Rac1 GTPase in melanoblast motility and melanoma invasion
and metastasis
IS6 Arnoud Sonnenberg (Amsterdam, NL)
Regulation of hemidesmome dynamics during cell migration
OP14 Lionel Larue (Paris, France)
Lack of PTEN overcomes senescence barrier and promotes melanoma
metastasis on a NRAS background
10:30-11:00 Coffee break

11:00-12:30 4 th Session: From Structure to Function and Therapeutics:
Chairs: Robert Kelsh and Alessandra Napolitano
IS7 Eirikur Steingrimsson (Reykjavik, Iceland)
DNA binding specificity and restricted dimerization properties of the melanocyte
master regulator MITF
IS8 Olivier Michielin (Lausanne, Switzerland)
Rational design of new therapeutic strategies in stage IV melanoma
OP15 Francoise Degoul (Clermont-Ferrand, France)
Targeted radionuclide therapy for pigmented melanomas
OP16 Marie Dutreix (Paris, France)
New DNA repair inhibitors for Melanoma treatment: Dbait
OP17 Christine Grill (Reykjavik, Iceland)
MITF and IRF4 regulate human pigmentation
12:30-14:00 Lunch buffet and poster viewing
14:00-14:45 Keynote lecture: Microenvironment and Cancer
Chair: Richard Marais
KL1 Doug Hanahan (Lausanne, Switzerland) Melanoma and its environment
14:45-16:30 5 th Session: Stem Cells
Chairs: Jo Lambert and Colin Goding
IS9 Dot Bennett (London, UK)
Overview: stem cell concepts for melanocytes and melanoma
IS10 Yann Barrandon (Lausanne, Switzerland)
Keratinocyte stem cells
IS11 Colin Goding (Oxford, UK)
A key chromatin remodelling complex controls MITF expression and activity.
OP18 Severine Tabone-Eglinger (Geneva, Switzerland)
Membrane-bound KitL: kinase dependent and independent functions through
the c-kit receptor to control stem cells
16:30-17:00 Coffee break
17:00-19:00 6 th Session: Hyperpigmentation:
Chairs: Heather Etchevers and Dot Bennett
IS12 Jo Lambert (Gent, Belgium)
The melanocyte and its microenvironment : lessons from the clinic
IS13 Alain Taïeb (Bordeaux, France)
Dermal influences on epidermal pigmentation
IS14 Jose Carlos Garcia-Borron (Murcia, Spain)
Relationships of melanocortin 1 receptor signaling to the cAMP and mitogen-activated
protein kinase ERK1 and ERK2 pathways in heterologous and human melanoma cells
OP19 Andre Furger (London, UK)
MC1R poly(A) site and its role to regulate intergenic splicing and the expression of
chimeric MC1R-Tubulin beta III receptors
OP19bis Kasraee Behrooz (Geneva, Switzerland)
Evaluation of a novel colorimetric technology for discrimination and measurement
of cutaneous pigmentation and erythema

19:30 Gala-dinner
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3 rd day (Thursday, 13. September 2012)
08:30-10:30 7 th Session: The Melanocyte Environment in Health and Disease:
Chairs: Eugene Healy and Jose-Carlos Garcia-Borron
IS15 Eugene Healy (Southampton, UK)
Regulating keratinocyte cancer; battles within battles
IS16 Mauro Picardo (Rome, Italy)
Vitiligo, a multi-pathways process: a metabolic view
IS17 Bassam Janji (Luxembourg City, Luxembourg)
Influence of hypoxia on melanoma susceptibility to CTL mediated lysis
OP20 Muriel Cario-André (Bordeaux, France)
Fibroblasts regulate both physiological and pathological skin pigmentation in
vitro and in vivo
OP21 Tag S. Anbar (Al-Minya, Egypt)
Inflammatory cells in vitiligo: is it a rare finding?
10:30-11:00 Coffee break
11:00-12:30 8 th Session: Melanoma and the Role of the Microenvironment:
Chairs: Laura Machesky and Luisa Lanfrancone
IS18 Elisabeth Patton (Edinburgh, UK)
A novel role for the phosphatase PRL-3 in melanocyte regeneration
IS19 Anja Bosserhoff (Regensburg, Germany)
miRNAs in malignant melanoma development and progression
IS20 Corine Bertolotto (Nice, France)
MITF controls the senescence and stem cell-like properties of melanocyte cells
OP22 Ruth Halaban (New Haven, USA)
The Landscape of Mutations in Melanomas Revealed by Exome Sequencing
12:30-14:00 Lunch and poster viewing
13:00-14:00 General Assembly of the ESPCR
14:00-16:00 8 th Session: Melanoma and the Role of the Microenvironment:
Chairs: Anja Bosserhoff and Eirikur Steingrimsson
IS21 Luisa Lanfrancone (Milan, Italy)
Molecular determinants of cell invasion in melanoma: in vitro and in vivo studies
IS22 Lukas Sommer (Zürich, Switzerland)
Common regulators of neural crest stem cells and melanoma formation
IS23 Adam Hurlstone (Manchester, UK)
Novel determinants of melanoma progression from zebrafish

IS24 Donata Rimoldi (Lausanne, Switzerland)
Development of melanoma: lessons from next generation sequencing
OP23 Nallet-Staub et al (Paris, France)
Characterization of the Hippo Pathway in Melanoma: Focus on the effectors
YAP and TAZ
16:00-16:30 Coffee break
16:30-17:30 8 th Session: Melanoma and the Role of the Microenvironment:
Chairs: Marie-Dominique Galibert and Alain Mauviel
IS25 Jose Neptuno Rodriguez Lopez (Murcia, Spain)
Methotrexate promotes MITF-driven phenotype-switching in melanomas
IS26 Manfred Schartl (Würzburg, Germany)
Use of the medaka fish model to identify conserved molecular pathways in
melanoma
OP24 Catherine André (Rennes, France)
Melanoma in predisposed dog breeds: spontaneously occuring models for genetics
and therapies of human melanoma
17:30-18:00 Keynote Lecture
Chair: Bernhard Wehrle-Haller
KL2 Richard Marais (Manchester, UK)
BRAF in melanoma
18:00-18:15 Concluding remarks and welcome to the ESPCR2013
(Bernhard Wehrle-Haller and Graça Raposo)
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Additional abstracts:
IS20; MITF controls the senescence and stem cell-like properties of melanocyte cells
Bertolotto Corine
INSERM U1065, 151, Av. St Antoine de Ginestière, BP3194, 06204 Nice, France, bertolot@unice.fr
The transcription factor MITF is the master gene of the melanocyte lineage. Many reports sustain a
role for MITF in melanoma disease. In this regards, we recently identified a gain of function
mutation of MITF that predisposes to melanoma (MITF E318K ). We demonstrated in vitro that
MITF E318K increases the tumorigenic properties of melanocyte cells. Conversely, we showed that
the sustained inhibition of MITF triggers senescence of melanoma cells. These results indicate that
in some circumstances MITF can overcome the program of senescence and can favor melanoma
development. However, we and other also provide evidences that transient inhibition of MITF
increases the tumorigenic potential of melanoma cells. Indeed, we showed that MITF inhibition by
siRNA in human and mouse melanoma cells triggered an increase in the expression of the stem
cell markers Oct4 and Nanog. Further, low-MITF expressing cells are more prone to form tumors in
mice than high-MITF expressing cells. A slow-growing population with low-Mitf level and high
tumorigenic potential also exists spontaneously in human melanoma samples. Ablation of this
population dramatically decreases tumor formation. I will give an overview of the complex role of
MITF that appears at the heart of programs controlling the senescence and stem cell-like properties
of melanocyte cells.

IS23; Novel determinants of melanoma progression from zebrafish
Adam Hurlstone
University of Manchester, Manchester, UK
Frequent driver mutations detected in BRAF, NRAS, CKIT, GNAQ and ERBB4 in melanoma,
malignant melanocyte neoplasms, present opportunities for personalized medicines in the form of
drugs targeting these oncogene products or their downstream signalling components like MEK.
While promising effects have recently been achieved in the clinic for advanced melanoma
particularly for small molecule inhibitors of BRAF, relapse appears inevitable and spurs the search
for novel targets and mechanistic insight into resistance mechanisms. We and others have shown
that expressing BRAF and RAS driver mutations in zebrafish melanocytes also induces melanocyte
neoplasia with histological and molecular hallmarks of human melanoma. These animal models of
melanoma afford new opportunities to identify novel determinants of progression, which could
potentially be therapeutic targets. We identify the small GTPase Rac as a determinant of
progression. Transcriptome profiling is also providing a systems view of an expression profile
associated with progression that again is likely to harbor novel therapeutic targets.
35(bis). Comparison of the skin depigmenting effect of Cysteamine cream with
Hydroquinone in black guinea pig skin
Pourahmadi M. 1 , Ahmadi S. 1 , Bazafkan S. 1 , Zahraie N. 1 , Hsu C. 2
1 Jahrom University Medical School, Jahrom, Iran, 2 Lignon Medical Center, Geneva, Switzerland
L-cysteamine (alpha-mercaptoethylamine) is a potent skin depigmenting agent known since more than
4 decades. However, due to its very offensive odor, it has never been used as a topical depigmenting
agent. Recently, a new technology has become available that permits to significantly reduce the odor of
L-cysteamine in topical preparations, making it utilizable as a topical depigmenting product. In this
study, we have compared the depigmenting effect of cysteamine cream with 4% topical hydroquinone
in black guinea pig model. Six black guinea pigs were treated once daily for 10 days on one of their
ears with cysteamine cream and on the contralateral ear with 4% hydroquinone cream. Six control
guinea pigs were treated with the vehicle alone. Dermacatch® colorimetry was performed at the
beginning and at the end of the study. Biopsies were taken at the end of the trial and were processed
for H&E, Fontana-Masson, Melan-A, HMB-45 and spectrophotometric melanin quantification in the
splitted epidermis. Dermacatch colorimetry and melanin quantitation in the splitted epidermis confirmed
the significant depigmenting effect of both formulations. Histologic and immunohistologic examinations
confirmed the depigmenting effect of cysteamine cream as well as hydroquinone and provided clues to
the understanding of the mechanism of action of cysteamine. No statistically significant difference was
found between the epidermal melanin content in cysteamine treated samples and hydroquinone treated
skin. Cysteamine cream appears to be a potent depigmenting cream with an efficacy comparable to
hydroquinone in vivo and might be considered for the treatment of skin hyperpigmentary disorders in
future.
72. RNA sequencing and molecular characterization of BRAF V600E melanomas in zebrafish
Amy Capper 1 , James Lister 2 , Zhiqiang Zeng 1 , Jenny Richardson 1 , Marie Mathers 3 , Ian
Jackson 1 , E. Elizabeth Patton 1 .
1. MRC Human Genetics Unit, Crewe Road, Edinburgh, UK. 2. Department of Human and Molecular
Genetics and Massey Cancer Center, Virginia Commonwealth University School of Medicine, Richmond,
Virginia, USA. 3. National Heath Service, Western General Hospital, Crewe Road, Edinburgh, UK.
BRAF V600E expression in zebrafish melanocytes leads to expansion of melanocyte number, similar
to nevi. We have found that additional mutations, such as in the tumour suppressor p53 or in the
master melanocyte regulator mitf lead to melanoma development. We have collected over 80
zebrafish melanomas and used immunohistochemistry to show that these two different mutations
cooperate with BRAF V600E to confer distinct histopathological features, representing two melanoma
subtypes. To understand the differences between these subtypes, we are carrying out RNAsequencing
and exome sequencing (in collaboration with the Sanger Institute) to identify genetic
changes and transcriptome signatures representative of these two subtypes. Transcriptome
sequencing of a total of 34 zebrafish melanoma samples is being carried out and will be analysed
to identify differential expression of key genes between tumour subtypes, alternative splicing and
gene fusion events.

List of posters (N° and presenter underlined):
1 st 
Session
–
Development
of
the
Melanocyte
1. Tomoko Adachi 1,3 , Yusuke Nagao 1,2 , Vicky Chau 3 , Masahiko Hibi 1,2 , Robert N.
Kelsh 3 , Hisashi Hashimoto 1,2
Sox5 regulates the development of neural crest-derived pigment cells in medaka and
zebrafish
2. (OP5) Toyoko Akiyama 1 , Ai Shinomiya 1 , Keiji Kinoshita 2 , Makoto Mizutani 2 , Yoichi Matsuda 2
Effects of pigmentation related genes on the fibromelanosis phenotype in Silky chickens
3. Johansen, P. 1 , Andersen, J.D. 1 , Delgado, M.C. 1 , Christoffersen, S.R. 2 , Harder,
S. 2 , Paulsen, R.R. 2 , Børsting, C. 1 and Morling, N. 1
The genetics of human eye colour using a novel eye colour classification method
4. (OP2) Verena A. Kottler, Detlef Weigel, Christine Dreyer
Kita and csf1ra mutants provide novel insights into pigment pattern formation in the
guppy, Poecilia reticulata
5. (OP1) Mitch Levesque 1,2 , Johannes Junker 1 , Christian Soellner 1 , Georg Otto 1 , Karsten
Borgwart 1 , Christianne Nüsslein-Volhard 1
Tissue-specific transcriptomes and extracellular protein interactions identify gene
regulatory networks involved in neural crest development and zebrafish pigmentation
6. (OP4) Edouard Reyes-Gomez, Nelly da Silva, Stéphanie Gadin-Czerw, Jean-Jacques
Panthier, Geneviève Aubin-Houzelstein
Sbno2 overexpression in Dct-expressing cells alters melanocyte and nerve development
in mice
7. (OP3) Davide Seruggia 1 , Lluís Montoliu 1,2
The nuclear structure of the mouse tyrosinase locus
8. J. Djian-Zaouche 1, 2 , C. Campagne 1, 2 , G. Egidy 1, 2, 3 , S. Jule 1, 2 , E. Reyes-Gomez 1,
2 , S. Gadin-Czerw 1, 2 , F. Bernex 1, 2 , A. Louise 3 , F. Relaix 3 , M. Buckingham 3 , F.
Beermann 4 , J.J. Panthier 3 1, 2, 3
and G. Aubin-Houzelstein
Pax3 GFP : a new reporter for the melanocyte lineage and melanoma cells in the mouse
9. F. Belaubre, S. Poigny, E. Questel, S. Julie, N. Castex-Rizzi, S. Bessau-Touya
A Unique Three-Compound Association to improve Melasma and Solar Lentigo
Hyperpigmentation
10. Jens Hoffmann, Daniel Fuchs, Tanja Klein, Oliver Engelking & Horst W. Fuchs
Characterisation of a new melanocyte containing reconstructed epidermis
2 nd 
Session
–
The
Bright
and
the
Dark
Side
of
the
Sun
11. Seunghyun Bang, Myoung Shin Kim, Sora Choi, Jeong Hwan Kim 1 , Chong Hyun Won,
Mi Woo Lee, Jee Ho Choi, Kee Chan Moon, Sungeun Chang
Inhibition of laser-induced post-inflammatory hyperpigmentation by tranexamic acid: in
vitro study
12. Seunghyun Bang, Woo Jin Youn, Sora Choi, Kyunghyun Min, Chong Hyun Won, Mi
Woo Lee, Jee Ho Choi, Kee Chan Moon, Sungeun Chang
Human epidermal melanocytes express EGF receptor and respond to EGF via Erk
signaling
13. Marta Abrisqueta 1 , Concepción Olivares Sánchez 1 , Ana B Pérez-Oliva 1 , Cecilia
Herraiz 1 , José Carlos García-Borrón 1, 2 and Celia Jiménez-Cervantes 1, 2 .
Mahogunin Ring Finger 1 may contribute to MC1R-dependent ubiquitylation of β-arrestins
3 rd 
Session
–
Adhesion
and
Migration
of
Melanocyte
and
Keratinocyte

14. (OP14) A. Conde Perez 1 , C. Longvert 1 , G.Gros 1 , I. Puig 1 , F. Beermann 2 , Leon Van Kempen 3 ,
L. Larue 1
Lack of PTEN overcomes senescence barrier and promotes melanoma metastasis on
a NRAS background
15. (OP6) M. Domingues, F. Rambow, L. Larue and J. Bonaventure
Increased motility and invasiveness of melanoma cells overexpressing ICAT, an
inhibitor of β-catenin
16. Ino F. 1 , Kaya G. 2 , Piguet V. 3 , Fontao L. 1
Co-expression of Vimentin, Nestin and Plectin in melanomas
17. Valérie Petit* 1 , Fabrice Cordelières* 2 , Stuart Gallagher 1 , Olivier Debeir 3 and Lionel Larue 1
Automated cell tracking and analysis in phase contrast videos: Development of a java
software based on combined mean-shift processes
4 th 
Session
–
From
Structure
to
Function
and
Therapeutics
18. (OP15) Viallard C 1 , Bonnet M 1 , Besse S 1 , Cardamone, E 1 , Mishellany F 2 , Cayre A 2 , Labarre P 1 ,
Miot-Noirault E 1 , Chezal J-M 1 , Sun Y-S 3 , Dutreix M 4 , Degoul F 1
Targeted radionuclide therapy for pigmented melanomas
19. (OP16) Julian Biau 1,2 , Flavien Devun 3 , Amélie Crozet 1,3 , Cécile Laurent 1 , Mano Sayarath 3 ,
Jian-sheng Sun 3 , Simon Saule 1 , and Marie Dutreix 1
New DNA repair inhibitors for Melanoma treatment: Dbait
20. Christine Grill 1 , Kristín Bergsteinsdottir 1 , Margret H. Ögmundsdottir 1 , Veronique
Pingault 2 and Eirikur Steingrimsson 1
Functional analysis of MITF mutations associated with Melanoma and with
Waardenburg and Tietz syndromes
21. Maria S. Correia 1,2 , Giulia Bolasco 3,4 , Alistair N. Hume 3,5 , Abul K. Tarafder 1 , Francisco
Pereira 1 , Clare E. Futter 7 , Duarte C. Barral 1 and Miguel C. Seabra 1,2,3
Molecular characterization of melanin uptake and processing by keratinocytes
22. Anca Filimon, Sabina Zurac 1 , Gabriela Negroiu
The study of TRP-2 antigen expression in cell populations of melanocytic lesions revealed
molecular events with possible implications in melanoma diagnosis and prognosis
23. (OP17) Christian Praetorius 1 , Christine Grill 1 , Alex Metcalf 2 , Alexander Schepsky 1 , Aaron Smith 2 ,
Kathleen Robinson 3 , Eric van Otterloo 4 , David Fisher 3 , Robert Cornell 4 , Rick Sturm 2 , and
Eiríkur Steingrímsson 1
MITF and IRF4 regulate human pigmentation
24. Hiroko Isoda 1,2 , Myra O. Villareal 2 , Junkyu Han 1,2 , Hideyuki Shigemori 1 and Abdelfatteh
EL Omri 1
Hair growth promotion and melanogenesis regulatory effects of Erica multiflora extract
25. (OP7) Cristiana F. Pires 1,2 , Inês P. Rodrigues 1 , Sara Maia 1 , Martim D. Portal 1 , José S.
Ramalho 1 , Miguel C. Seabra 1,3
Optimization of a Retinal Pigment Epithelium differentiation protocol driven by ectopic
expression of eye transcription factors
26. Marieke Raaijmakers 1 , Daniel Widmer 1 , Tania Herrera de Rolo 1 , Reinhard Dummer 1 ,
Mitch Levesque 1
Exploring phenotypic heterogeneity in melanoma cultures
27. Jung-Min Shin 1 , Mi-Yoon Kim 1 , Hye in Choi 1 , Kyung-Cheol Sohn 1 , Chang Deok Kim 1 ,
Jeung-Hoon Lee 1 , Tae-Jin Yoon 2 , Young Lee 1
Effect of Nrf2/Keap1 pathway on melanogenesis
28. L. Ondrušová, J. Réda, J. Vachtenheim, Z. Tuháčková
Involvement of c-Src activity in the mTOR-dependent oncogenicity of melanoma cells

29. Myra O. Villareal 1 , Junkyu Han 1,2 , Kenjiro Ikuta 3 , Hideyuki Shigemori 2 , and Hiroko Isoda 1,2
The role of MAPK and cAMP pathways in the melanogenesis and cell differentiation
effects of hirsein A in B16 murine melanoma cells
5th
Session
–
Stem
Cells
30. (OP18) Séverine Tabone-Eglinger, Nicole Aebischer, Monique Wehrle-Haller, Marie-Claude
Jacquier, David Boettiger and Bernhard Wehrle-Haller
Membrane-bound KitL: kinase dependent and independent functions through the c-kit
receptor to control stem cells
31. (OP8) L. Larribère, J. Utikal
Human iPS cell-based modeling of hyperpigmentation in Neurofibromatosis type 1 (NF1)
32. Daniel Zingg 1 , Olga Shakhova 1 , Lynda Chin 2 , Friedrich Beermann 3 , Haruhiko Koseki 4 ,
Lukas Sommer 1
Role of the epigenetic modifier EZH2 in melanoma
6th
Session
–
Hyperpigmentation
33. (OP19) Martin Dalziel, Marina Kolesnichenko, Nuno Nunes, Ricardo Pires das Neves,
Francisco Iborra, Colin Goding and André Furger
MC1R poly(A) site and its role to regulate intergenic splicing and the expression of
chimeric MC1R-Tubulin beta III receptors
34. Hsu C. 1 , Ahmadi S. 2 , Pourahmadi M. 3 , Ali Mahdi H. 4
Cysteamine cream as a new skin depigmenting product
35. Ahmadi S. 1 , Pourahmadi M. 1 , Aslani F.S. 2 , Kasraee B. 3
Topical isonicotinic acid hydrazide (isoniazid) as a new skin depigmenting compound
35. (bis) Pourahmadi M. 1 , Ahmadi S. 1 , Bazafkan S. 1 , Zahraie N. 1 , Hsu C. 2
Comparison of the skin depigmenting effect of Cysteamine cream with Hydroquinone in
black guinea pig skin
36. (OP19 bis ) Baquié Mathurin 1 , Kasraee Behrooz 2
Evaluation of a novel colorimetric technology for discrimination and measurement of
cutaneous pigmentation and erythema
37. (OP9) Chandana Basu Mallick 1,2
An insight on SLC24A5 gene and its role in human skin color variation
38. Jin Hee Kim *
Down-regulation of melanin synthesis by ori2 and its derivatives in Melan-a cells
7 th 
Session
–
The
melanocyte
environment
in
health
and
disease
39. (OP21) Tag S. Anbar, 1 ; Ibrahim El-Ghareeb, 2 ; Magda I. Assaf, 3 ; Hamza Abdel-Raouf,; Sherif S.
Awad,; Amal T. Abdel-Rahman 1 ; Maha H. Ragaie 1 ; Waleed M. Albalat 2
Inflammatory cells in vitiligo: is it a rare finding?
40. Arunachalam M, Colucci R, Conti R, Dragoni F, Galeone M, Moretti S.
Vitiligo and Psoriasis Comorbidity - a case control study in Italian patients
41. Barbara Bellei, Angela Pitisci and Mauro Picardo
Inhibition of Melanogenesis by the Pyridinyl Imidazole Class of Compounds: Possible
Involvement of the Wnt/β-catenin Signaling Pathway
42. (OP20) Cario-Andre Muriel 1,2,3 , Khaled Ezzedine 1,2,3 , Pain Catherine 1,2 , Guyonnet-Dupérat
Véronique 4 , Bibeyran Alice 4 , Taieb Alain 1,2,3
Fibroblasts regulate both physiological and pathological skin pigmentation in vitro and
in vivo
43. Catherine Pain 1,2,3 , Laila Benzekri 4 , Yvon Gauthier, Khaled Ezzedine 1,2,3 , Alice
Bibeyran 5 , Véronique Guyonnet-Dupérat 5 , Isabelle Lamrissi-Gracia 1,2, ,Alain Taieb 1,2,3
and Muriel Cario-André 1,2,3
Study of CCN3 (Nov) expression in normal skin and vitiligo skin

44. Maria Lucia Dell’Anna, Daniela Kovacs, Giorgia Cardinali Monica Ottaviani, Mauro
Picardo
Vitiligo pathogenesis: are mitochondria relevant players?
45. Duval C, Cohen-Dellarre C, Chagnoleau C, Bernerd F
Demonstration of the role of dermal fibroblasts in regulating pigmentation by means of
a functional full thickness pigmented skin model
46. Jennifer Allouche 1 , Lionel Larribère 2 , Gilles Lemaitre 1 , Xavier Nissan 1 , Marc
Peschanski 1 , Christine Baldeschi 1
Modeling pigmentary defects of neurofibromatosis type I using embryonic stem cells
47. Christian Muteba Baseke
Epidemiology study of vitiligo in three private hospitals in Kinshasa About 25 Cases
48. Hye-In Choi 1 , Mi Yoon Kim 2 , Jung-Min Shin 2 , Gue-In Choi 1 , Hyungwon Kim 1 , Kyung-
Cheol Sohn 2 , Dae-Kyoung Choi 2 , Chang Deok Kim 2 , Jeung-Hoon Lee 2 , Young Ho Lee 1
The role of melanosome in human keratinocytes
49. Colucci R. *, Benvenga S. § , Arunachalam M.*, Dragoni F. *, Galeone M*. Lotti F. δ , Lotti
T. # , Moretti S*.
Increased thyroid hormone autoantibodies in a group of non-segmental vitiligo patients-
Preliminary results
50. Laurence Denat, Laurent Marrot, Christophe Jones, Jean-Roch Meunier
Characterization at the transcription level of metabolic equipment of normal human
melanocytes and keratinocytes in culture
51. Duval C, Cohen-Dellarre C, Chagnoleau C, Bernerd F
Demonstration of the role of dermal fibroblasts in regulating pigmentation by means of
a functional full thickness pigmented skin model
52. Agustín España 1 , Celia Jiménez-Cervantes 1, 2 , André Furger 3 , José C García-Borrón 1, 2 ,
Cecilia Herraiz 1 .
Functional characterization of MC1R-TUBB3 intergenic splice variants
53. Mi Yoon Kim 1 , Jung-Min Shin 1 , Hye-In Choi 2 , Dae-Kyoung Choi 1 , Kyung-Cheol Sohn 1 ,
Jeung-Hoon Lee 1 , Tae-Jin Yoon 3 and Chang Deok Kim 1
Effect of etopic expression of tyrosinase on wound healing
54. Daniela Kovacs 1 , Hamza Abdel-Raouf 2 , Mohamed Al-Khayyat 2 , Eman Abdel-Azeem 2 ,
Pietro Donati 1 , Mauro Picardo 1 , Tag S. Anbar 2 .
Melanocyte characterization in vitiligo punch grafting
55. JY Kim, JY Shin, MR Kim, SK Hann, SH Oh
siRNA-mediated knockdown of COX-2 in melanocytes suppresses melanogenesis
56. Chieh-Shan Wu 1,2,3 , Mayumi Komine 3,4 , Seiki Fujimoto 5 , Hanako Ohmatsu 3 , Kanako
Kikuchi 3 , Hisao Enami 5 , Yayoi Tada 3 , Hsin-Su Yu 2 , Mamitaro Ohtsuki 4 , Kunihiko Tamaki 3
Narrow-band UVB induced skin pigmentation is correlated with epidermal
keratinocyte proliferation: induction of keratinocyte proliferation as a novel
strategy for the treatment of vitiligo.
8 th 
Session
–
Melanoma
and
its
environment
57. (OP24) Edouard Cadieu 1,2* , Marc Gillard 1,2* , Clotilde De Brito 1,2 , Jérome Abadie 3 , Béatrice
Vergier 4 , Anne Sophie Guillory 1,2 , Patrick Devauchelle 5 , Fréderique Degorce 6 , Laëtitia
Lagoutte 1,2 , Benoit Hédan 1,2 , Marie-Dominique Galibert 1,2 and Catherine André 1,2
Melanoma in predisposed dog breeds: spontaneously occuring models for genetics and
therapies of human melanoma
58. (OP10) S. Druillennec 1 , C. Dorard 1 , A. Valluet 1 , C. Barbotin 1 , M. Larcher 1 , F. Beermann 2 , M.
Baccarini 3 , L. Larue 1 , A. Eychène 1 .
Raf signaling in melanocyte and melanoma development

59. (OP22) Michael Krauthammer 1 , Yong Kong 2,3 , Byung Hak Ha 4 , Perry Evans 1 , Antonella
Bacchiocchi 5 , James P McCusker 1 , Elaine Cheng 5 , Matthew J Davis 4 , Gerald Goh 6, 7 ,
Murim Choi 6, 7 , Stephan Ariyan 8 , Deepak Narayan 8 , Ken Dutton-Regester 9,10 , Ana
Capatana 1 , Edna C Holman 5 , Marcus Bosenberg 5 , Mario Sznol 11 , Harriet M Kluger 11 ,
Douglas E Brash 5,6,12 , David F Stern 1 , Miguel A Materin 13 , Roger S Lo 14 , Shrikant Mane 6,15 ,
Shuangge Ma 16 , Kenneth K Kidd 6 , Nicholas K. Hayward 10 , Richard P Lifton 6,7 , Joseph
Schlessinger 4 , Titus J Boggon 4 , Ruth Halaban 5*
The Landscape of Mutations in Melanomas Revealed by Exome Sequencing
60. Fabrice Journe 1,# , Petra El Hajj 1,# , Murielle Wiedig 1 , Ioanna Laios 2 , François Salès 1 ,
Marie-Dominique Galibert 3 , Léon C van Kempen 4 , Bassam Badran 5 , Denis Larsimont
2 , Ahmad Awada 6 , Ghanem Ghanem 1
Tyrosinase-related protein 1 mRNA expression in lymph node metastases predicts
disease-free and overall survival in high risk melanoma patients
61. (OP23) Flore Nallet-Staub, Cristèle Gilbert, Sophie Dodier, Véronique Bataille, Marius Sudol,
Ling Li, Meenhard Herlyn, and Alain Mauviel
Characterization of the Hippo Pathway in Melanoma: Focus on the effectors YAP and TAZ
62. Murielle Wiedig 1,# , Mimoune Berehab 1,# , Batiste Leroy 2 , Ruddy Wattiez 2 , Renato
Morandini 1 , Ahmad Awada 3 , Ghanem Ghanem 1 , Fabrice Journe 1
Proteomic analysis of V600E BRAF versus WT BRAF melanoma cell lines
63. (OP11) K. Brown, V. Sherwood.
Investigating heterogeneous cell populations in melanoma tumours
64. Golovko, Anna 1 , Susanne Brükner 1 , Jörn Völker 1 , Veronica Höiom 2 , Johan
Hansson 2 , Andersson, Leif 1,3
Exon resequencing of PMEL17 in swedish melanoma patients
65. Delphine Javelaud, Dominique Dewolf, Elena Grigorenko and Alain Mauviel
Simultaneous TaqMan® Q-PCR quantitation of hundreds of cancer-related genes in
melanoma cell lines using the OpenArray® technology
66. Delphine Javelaud, Dominique Dewolf, Elena Grigorenko and Alain Mauviel
Melanoma cell miRnome analysis using the TaqMan® OpenArray® technology:
Modulation by TGF-β
67. Marie-Jeanne Pierrat, Véronique Marsaud, Alain Mauviel, and Delphine Javelaud
Molecular Mechanisms underlying Inhibition of Microphthalmia-associated Transcription
Factor (MITF) Expression by Transcription Factor GLI2 and Transforming Growth Factor-β
68. Michal Sarna 1 , Anna Pilat 2 , Magdalena Olchawa 2 , Andrzej Zadlo 2 , Paraskevi
Gkogkolou 3 , Kvetoslava Burda 1 , Markus Bohm 3 , Tadeusz Sarna 2
Melanin modifies nanomechanical properties of melanoma cells
69. Zied Boudhraa 1 , Fabien Rondepierre 1 , Anna Trzeciakiewicz 1 , Christine Merle 2 ,
Elisabeth Miot-Noirault 1 , Jean-Michel Chezal 1 , Michel D’Incan 1 , Bernadette Bouchon 1
and Françoise Degoul 1
Annexin A1 N-terminal peptide in melanoma dissemination
70. (OP12) Phil F Cheng, Daniel S Widmer, Benedetta Belloni, Marieke Raaijmakers, Ossia M
Eichhoff, Keith S Hoek, Reinhard Dummer, Mitchell P Levesque
DNA methylation in melanoma phenotype switching
71. (OP13) Mohammad Krayem, Fabrice Journe, Renato Morandini, Ahmad Awada*,
Ghanem Ghanem
Vemurafenib stimulates pigmentation in V600E BRAF melanoma cell lines through MITF
stimulation and cell differentiation
72. Amy Capper 1 , James Lister 2 , Zhiqiang Zeng 1 , Jenny Richardson 1 , Marie Mathers 3 , Ian
Jackson 1 , E. Elizabeth Patton 1
RNA sequencing and molecular characterization of BRAF V600E melanomas in zebrafish
Venue and Access: