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Bacterial Meningitis

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Diagnosis (cont.)<br />

• METHODS FOR DETECTING BACTERIA IN CSF:<br />

• Gram staining<br />

• Acridine orange stain<br />

• Fluorochrome stain (bacteria appear bright red)<br />

• More sensitive than gram stain<br />

• Reduction in the time of examination of CSF smear<br />

• Requires fluorescence microscope<br />

• Wayson stain<br />

• Simple & sensitive stain for screening CSF smears for<br />

bacteria<br />

• Bacteria appear dark blue

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