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25 uV<br />
cps<br />
175<br />
150<br />
125<br />
100<br />
cps<br />
75<br />
50<br />
25<br />
cps<br />
175<br />
150<br />
125<br />
100<br />
cps<br />
175<br />
150<br />
125<br />
100<br />
175<br />
150<br />
125<br />
100<br />
100 G<br />
50<br />
25<br />
75<br />
50<br />
25<br />
80<br />
60<br />
40<br />
20<br />
75<br />
50<br />
25<br />
75<br />
0<br />
ADC1 B, raga 10mL tube (AXEL<br />
\11090220.D)<br />
5 10 15 20 25 30 35 40<br />
\<br />
min<br />
min<br />
INDUCED CHEMICAL DEFENSES IN CONIFERS 15<br />
E. coli control<br />
G<br />
GG<br />
PaIDS1<br />
G<br />
F<br />
PaIDS4<br />
GG<br />
PaIDS5/6<br />
F GG<br />
Standard<br />
10 20 30 40<br />
Retention time<br />
Fig. 1.8: Catalytic activities of the isoprenyl diphosphate synthases<br />
PaIDS1, PaIDS4, PaIDS5, and PaIDS6 after heterologous expression in E.<br />
coli. Products were measured by radio-gas chromatography (plotted in<br />
Bequerel, upper four panels) and identified by co-injection of nonradioactive<br />
terpene standards, detected via a thermal conductivity detector<br />
(plotted as detector response, bottom panel). The main products after acid<br />
hydrolysis are listed: G, geraniol; F, farnesol; and GG, geranylgeraniol.<br />
Bacteria containing only the expression vector without a isoprenyl<br />
diphosphate synthase sequence showed no enzyme activity (top panel).
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