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Pentose-Fermenting Zymomonas mobilis - EERE - U.S. Department ...

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Concentration (g/L)<br />

Adaptation of <strong>Zymomonas</strong> 39676(pZB4L) to above 60% (v/v) OL Hydrolyzate<br />

50<br />

40<br />

30<br />

20<br />

10<br />

0<br />

CSL=2% (v/v) D = 0.015 (1/hr)<br />

Strain Improvement through Adaptation<br />

Feed Mixture: Glucose (8 g/L) + Xylose (40 g/L)<br />

T=33C, pH=5.8<br />

glucose<br />

xylose<br />

ethanol<br />

% hydrolyzate<br />

0 10 20 30 40 50 60 70<br />

Time (Days)<br />

80<br />

70<br />

60<br />

50<br />

40<br />

30<br />

20<br />

10<br />

0<br />

Hydrolysate (%, V/V)<br />

Concentration (g/L)<br />

50<br />

40<br />

30<br />

20<br />

10<br />

0<br />

Comparison of Adapted and Parent Strain 39676(pZB4L) Grown on cCSL (2%),<br />

60% Sawdust Hydrolysate at pH 5.8, T 30°C, Initial Sugar Conc. (G:X 1%:5%)<br />

Glucose<br />

Xylose<br />

Ethanol<br />

Ethanol<br />

Adapted Parent<br />

Ethanol

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