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Pentose-Fermenting Zymomonas mobilis - EERE - U.S. Department ...

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Metabolic Engineering Tools<br />

• Transcriptomics<br />

� Examine gene expression at global level<br />

• Metabolomics<br />

� Examine metabolites at global level<br />

• High-throughput Enzyme Assays<br />

� Compare strains<br />

� Compare activities at different stages of growth<br />

� Compare activities in different growth conditions<br />

� Develop improved enzymes<br />

• Mathematical approaches<br />

− Metabolic flux analysis<br />

− Kinetic modeling

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