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TDS - BD Biosciences

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Materials Provided<br />

Page 2 of 8<br />

(Version 030626)<br />

MicroFlow BASIC Kit (Mouse)<br />

Kit Component Quantity a Storage Condition<br />

Solution A (fixative) b 250 ml (10 - 25 ml) Room temperature<br />

Solution B (anticoagulant) 30 ml 4 °C<br />

15-ml conical polypropylene tubes 120 Room temperature<br />

Shipping container c 1 Room temperature<br />

Small clear plastic bag for shipping protocol 1<br />

Large clear plastic bags for shipping samples 6 Room temperature<br />

Protocol Acceptance Form 1 Room temperature<br />

a. Sufficient materials are provided to collect 60 blood samples. Enough fixative is included to fix each sample in duplicate. (Duplicate<br />

(backup) samples are an important precaution against shipping problems.)<br />

b. Warning! Methanol. Flammable liquid and vapor. Toxic. See MSDS.<br />

c. It is important to save the insulated shipping box in which the kit was received. This insulated box should be used for shipping fixed<br />

blood samples to Litron. When filled to capacity with dry ice, this container is designed to ensure that 50 – 60 fixed samples will<br />

remain sufficiently cold for 48–72 hours, which is sometimes necessary due to shipping delays.<br />

Additional Materials Required<br />

• –75 to –85 °C freezer (a chest freezer is preferred)<br />

• 4 °C refrigerator<br />

• Micropipet (20–1000 µl)<br />

• Standard pipet tips<br />

First-Time Users<br />

• Microcentrifuge tubes<br />

• Dry ice for shipment of samples<br />

• Shipping forms for overnight delivery service<br />

• Blood collection supplies<br />

We recommend reading the entire instruction manual before performing these<br />

procedures. First-time users of this kit should perform the following steps on five to<br />

ten test samples to practice fixing and shipping the blood samples to Litron.<br />

Please do not deviate from the procedures described in this manual. It is important that<br />

these steps are followed exactly using the reagents supplied with this kit in order to<br />

achieve accurate results. If you have questions, please contact Litron Laboratories by<br />

calling (585) 442-0930, faxing us at (585) 442-0934, or sending an email to<br />

info@litronlabs.com.<br />

Before analysis can be<br />

initiated, a Protocol<br />

Acceptance Form (see<br />

Materials Provided, page 2)<br />

must be filled out and<br />

returned to Litron<br />

Laboratories, preferably with<br />

the fixed blood samples.

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