Fonseca et al., Supplementary Information FRAP data analysis

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184648, Figure S5, Fonseca 50 pmol 10 pmol 2 pmol Ponceau S (50 pmol) H3 unmodified H3 S28ph H3K27me3 H3K27meS28ph H3K27me2S28ph H3K27me3S28ph H3K9me3S10ph
Section Variable ID Variable NB NBmet SOP SOPmet pIIa pIIb NB Nbmet SOP SOPmet pIIa pIIb NB Nbmet SOP SOPmet pIIa pIIb Image-based parameters Kinetic parameters Quantification Modeling parameters PH::GFP PC::GFP A Volume (µm 3 ) 239.55 ± 26.43 682.99 ± 97.67 149.26 ± 12.12 752.52 ± 31.53 72.71 ± 2.65 53.86 ± 4.40 176.33 ± 9.56 726.72 ± 74.88 171.60 ± 5.94 590.66 ± 37.02 97.15 ± 11.59 68.62 ± 9.76 B # GFP 139409 ± 16220 74930 ± 8811 119147 ± 25089 133038 ± 22613 37372 ± 2367 25656 ± 2059 73350 ± 8201 118877 ± 12065 37461± 3090 39228 ± 3145 20902 ± 2222 14749 ± 1484 C µM GFP 0.98 ± 0.06 0.19 ± 0.03 1.36 ± 0.39 0.29 ± 0.04 0.86 ± 0.02 0.80 ± 0.01 0.70 ± 0.08 0.27 ± 0.02 0.36 ± 0.02 0.11 ± 0.01 0.36 ± 0.03 0.36 ± 0.03 D # end 24369 ± 2725 39494 ± 4008 20359 ± 1679 21320 ± 1709 11360 ± 1208 8015 ± 807 E µM end 0.23 ± 0.03 0.09 ± 0.01 0.20 ± 0.01 0.06 ± 0.002 0.20 ± 0.01 0.20 ± 0.02 F # end yw 40615 ± 9306 65823 ± 14762 48474 ± 13310 50762 ± 13904 27048 ± 7646 19083 ± 5355 G µM end yw 0.38 ± 0.09 0.15 ± 0.03 0.48 ± 0.13 0.14 ± 0.04 0.48 ± 0.13 0.48 ± 0.13 H Radius (µm) 2.27 4.38 2.86 3.43 2.28 2.28 2.99 3.45 2.91 6.36 2.37 2.19 4.19 8.18 2.91 5.52 2.78 2.80 I Df 1 (µm 2 .s -1 ) 1.05 ± 0.17 1.26 ± 0.15 0.76 ± 0.23 1.52 ± 0.56 1.12 ± 0.31 1.11 ± 0.43 5.10 ± 0.92 2.17 ± 0.45 3.00 ± 0.34 2.43 ± 0.30 2.42 ± 0.38 2.90 ± 0.35 11.43 ± 0.96 10.50 ± 0.45 8.17 ± 0.64 9.15 ± 0.50 6.82 ± 0.72 5.77 ± 0.61 J Df 2 (µm 2 .s -1 ) 1.14 ± 0.07 1.05 ± 0.06 0.68 ± 0.05 0.77 ± 0.06 0.57 ± 0.04 0.48 ± 0.04 3.41 ± 0.41 3.13 ± 0.38 2.80 ± 0.17 2.53 ± 0.15 2.33 ± 0.14 1.97 ± 0.12 K k off (s -1 ) 0.23 ± 0.05 0.002 ± 0.0007 0.10 ± 0.01 0.24 ± 0.06 0.15 ± 0.01 0.13 ± 0.01 2.19 ± 0.75 0.30 ± 0.18 0.72 ± 0.40 0.002 ± 0.0007 0.24 ± 0.11 0.27 ± 0.14 L k* on (s -1 ) 0.10 ± 0.04 0.0001 ± 0.00005 0.13 ± 0.04 0.15 ± 0.08 0.29 ± 0.05 0.27 ± 0.06 0.51 ± 0.38 0.06 ± 0.06 0.08 ± 0.08 0.0003 ± 0.00003 0.07 ± 0.04 0.04 ± 0.03 M Rtime (s) 4.26 607.72 9.77 4.17 6.52 7.78 0.46 3.35 1.39 431.33 4.10 3.72 N Fbound chr (%) 29.67 7.78 56.59 37.87 65.74 67.82 18.93 17.60 10.44 9.78 21.92 13.29 O Fbound total (%) 29.67 0.41 56.59 1.81 65.74 67.82 18.93 0.53 10.44 0.94 21.92 13.29 P # GFP bound 41360 ± 4812 310 ± 36 67421 ± 14197 2410 ± 410 24569 ± 1556 17399 ± 1396 13885 ± 1552 627 ± 64 3912 ± 321 367 ± 29 4581 ± 487 1960 ±197 Q #GFP + end bound 18498 ± 2068 835 ± 85 6038 ± 496 566 ± 45 7070 ± 752 3026 ± 304 R # end bound yw 7688 ± 860 347 ± 35 5062 ± 416 475 ± 38 5928 ± 630 2537 ± 255 S Volume chr (µm 3 ) 33.24 31.58 14.15 30.59 T # GFP chr 3,977 6,365 3,562 3,750 U Fbound chr (%) 8.73 12.84 35.74 48.32 V Fbound total (%) 0.46 0.61 1.07 4.62 W # GFP chr bound 347 817 1273 1,812 X k* 1 (s -1 ) 0.51 0.06 0.08 0.0003 0.06856 0.041193 Y k -1 (s -1 ) 2.19 0.3 0.72 0.002 0.24429 0.26871 Z # PC 40615 1216 48474 4633 27048 19083 AA Volume Cell (µm3) 176.33 14.15 171.60 30.59 97.15 68.62 AB Volume Chr (µm3) 176.33 14.15 171.60 30.59 97.15 68.62 AC # chromatin sites 80000 80000 80000 80000 80000 80000 AD Kd (µM) 11.23 35.04 18.28 36.67 7.71 14.13 GFPnls
Page 1 and 2: Fonseca et al., Supplementary Infor
Page 3 and 4: 2.5) Robustness of extracted k*on,
Page 5 and 6:   Fonseca, Steffen, Müller, Lu,
Page 13 and 14: NB PH NB PC SOP PH SOP PC 184648, F
Page 15: 184648, Figure S4, Fonseca

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