biotechnology symposium 2005 abstracts - Universität Leipzig

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26 PROTEIN ENGINEERING AND BIOANALYTICS 1.1 Creating novel proteins for therapeutic applications Rainer Rudolph Rudolph, Hauke Lilie, Ulrike Fiedler Recombinant proteins are by now widely used for the treatment of various diseases. For some therapeutic applications (e.g. cancer treatment) natural proteins offer only limited strategies for therapeutic intervention. For these applications, we designed novel modular proteins. We used polyionic adaptor peptides containing cysteine residues as highly specifi c heterodimerization motifs. Fusion of these polyionic peptides to both a protein module recognizing specifi c cell surface epitopes, and another module inducing cell death resulted in disulfi de-bonded immunotoxins, which possess a very high and specifi c cytotoxicity. The polyionic adaptor motif was also used to specifi cally modify virus like particles. As modular delivery systems, composed of a specifi c recognition domain, in vitro assembled capsids, and the therapeutic cargo (e. g. DNA) specifi c cell transformation could be achieved. Finally, novel binding molecules were created. For this purpose, two very stable human proteins, γ-crystallin from the eye lens, and ubiquitin were randomized in a solventexposed beta-sheet area. Applying suitable display and screening techniques specifi cally binding molecules can be isolated for both low molecular weight substances (e.g. estradiol) and globular proteins. Prof. Dr. Rainer Rudolph Martin-Luther-University Halle-Wittenberg Faculty of Mathematics, Natural Sciences and Technology Institute of Biotechnology Protein Technology E-Mail: rudolph@biochemtech.uni-halle.de www.biochemtech.uni-halle.de/proteintech/
1.2 Dark matter of the genome – The human genome contains thousands of non-coding RNAs Peter F. Stadler In contrast to the fairly reliable and complete annotation of the protein coding genes in the human genome, comparable information is lacking for non-coding RNAs. A computational screen of vertebrate genomes evaluating sequence conservation, secondary structure conservation, and thermodynamic stability of the putative RNA structures predicts more than 30,000 structured RNAs in the human genome, almost 1,000 of which are conserved across all vertebrates. Roughly a third is found in introns of known genes, a sixth are potential regulatory elements in untranslated regions, about half are located far away of any known gene. Only a small fraction of these sequences has been described previously. EST data demonstrate, however, that the majority of them is at least transcribed. We estimate, therefore, that the human genome contains at least as many RNA genes as protein coding genes. Prof. Dr. Peter F. Stadler <strong>Universität</strong> <strong>Leipzig</strong> Faculty of Mathematics and Computer Science Institute of Computer Science Bioinformatics E-Mail: studla@bioinf.uni-leipzig.de www.bioinf.uni-leipzig.de 27
Page 1: Center for Biotechnology and Biomed
Page 4 and 5: Editors Prof. Dr. Andrea A. Robitzk
Page 6 and 7: 3 BIOMEDICAL AND CELL ENGINEERING 3
Page 8 and 9: 4.17 Crystal structure of the 2-oxo
Page 10 and 11: 4.37 Purifi cation and structural c
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Page 18 and 19: 5.55 Molecular genetic diagnosis of
Page 20 and 21: 6.8 Evidence for abnormal iodinatio
Page 22 and 23: 7.3 Binding specifi city of peptide
Page 25: FOREWORD The Center for Biotechnolo
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98 MOLECULAR MEDICINE AND THERAPEUT
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166 BIOMEDICAL AND CELL ENGINEERING
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POSTERS 7. NANOBIOTECHNOLOGY AND NA
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7.2 Lipophilic nucleic acids as bui
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7.4 Nano- and microstructured surfa
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7.6 Proton microscopy - Quantitativ
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7.8 Investigation of the elastic pr
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POSTERS 8. BIOINFORMATICS
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8.2 Bio-Imaging platform LabImaging
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8.4 On the potential role of biomec
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8.6 Non-coding RNAs in Ciona intest
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8.8 Functional adaptations of dendr
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8.10 Multiple alignments of partial
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CENTER FOR BIOTECHNOLOGY AND BIOMED
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The following heads of research gro
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INDEX
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Dössel, Olaf 38 Drasdo, Dirk 205 E
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Lehmann, Jörg 65, 90 Lein, Sandro
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Spemann, Daniel 195 Stadler, Peter
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