biotechnology symposium 2005 abstracts - Universität Leipzig

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46 PROTEIN ENGINEERING AND BIOANALYTICS 4.3 Residue specifi c studies of NH exchange rates performed on ubiquitin Torsten Brand, Garreth A. Morris, Eurico J. Cabrita, Hans-Jörg Hofmann, Stefan Berger The aim of the present study was to establish a new method to invesigate NH exchange rates in proteins. These measurements are based on residue specifi c diffusion experiments and were demonstrated on human ubiquitin since it is a small protein (9 kDa, 76 residues) for which resonance assignment as well as NMR structures are available. For NMR studies a 1.8 mM solution of uniformly 13 C, 15 N-labelled ubiquitin in 90 % H 2 O, 10 % D 2 O was used. Experiments were carried out at 300 K on a Bruker Avance 700 spectrometer equipped with a cryo probe. In order to resolve most signals of the protein 1 H, 15 N-DOSY-HSQC spectra were recorded. Diffusion Ordered Spectroscopy was used to obtain an apparent diffusion coeffi cient D for each separated signal. Analysis of the apparent diffusion coeffi cients showed several “fast moving” NH protons. Subsequently, the exchange rates were derived directly from the decay data of the diffusion experiment by applying a model deduced from the assumption of two-site exchange with water and the “pure” diffusion coeffi cients of water and protein. Molecular modelling and MD studies have been performed in an attempt to predict exchange rates from structural or dynamical features of ubiquitin. Several parameters have been checked with respect to their utility for such a prediction, including static parameters like NH bond order, partial charge at the nitrogen and accessibility of the NH protons as well as the dynamics of hydrogen bond breaking. Whereas none of the parameters examined so far yielded a strong correlation, attempts to use a weighted combination of these parameters are topic of current research. Torsten Brand Universität Leipzig Faculty of Chemistry and Mineralogy Institute of Analytical Chemistry E-Mail: tbrand@rz.uni-leipzig.de www.uni-leipzig.de/~nmr/ANALYTIK/
4.4 Developmental pattern of tau isoforms in rat brain Torsten Bullmann, Max Holzer, Wolfgang Härtig, Nicolas Sergeant, Andre Delacourte, Rohan de Silva, Hiroshi Mori, Thomas Arendt Aggregates of hyperphosphorylated microtubule associated protein tau are found in Alzheimer’s disease and other neurodegenerative diseases called tauopathies. These aggregates differ in their tau isoform composition, phosphorylation of specifi c isoforms and fi lament confi rmation as well as in the affected cell types and regional distribution of lesions in brain. Some studies demonstrated a link between regional variation in tau isoform expression and susceptibility to neurodegeneration. Therefore distribution of tau protein isoforms in adult rat brain and during development was studied using recently avaiable specifi c antibodies, western blotting and RT-PCR. Single cell RT-PCR in combination with Laser microdissection may be a useful tool to study tau mRNA splicing in human brain to clearify whether a subset of neurons posses a specifi c tau protein isoform pattern that renders them highly vulnerable to degeneration. Torsten Bullmann Universität Leipzig Faculty of Medicine Paul Flechsig Institute of Brain Research Neuroanatomy E-Mail: bullmann@rz.uni-leipzig.de www.uni-leipzig.de/~pfi / 47
Page 1: Center for Biotechnology and Biomed
Page 4 and 5: Editors Prof. Dr. Andrea A. Robitzk
Page 6 and 7: 3 BIOMEDICAL AND CELL ENGINEERING 3
Page 8 and 9: 4.17 Crystal structure of the 2-oxo
Page 10 and 11: 4.37 Purifi cation and structural c
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Page 14 and 15: 5.22 Extended tropism of HIV vector
Page 16 and 17: 5.40 Quantitative assessment of the
Page 18 and 19: 5.55 Molecular genetic diagnosis of
Page 20 and 21: 6.8 Evidence for abnormal iodinatio
Page 22 and 23: 7.3 Binding specifi city of peptide
Page 25: FOREWORD The Center for Biotechnolo
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Page 35 and 36: 2.2 NAD + -dependent regulatory pro
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Page 41 and 42: 3.2 Generation of reliable diagnost
Page 43: 3.4 Dopaminergic neurons from midbr
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98 MOLECULAR MEDICINE AND THERAPEUT
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100 MOLECULAR MEDICINE AND THERAPEU
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166 BIOMEDICAL AND CELL ENGINEERING
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POSTERS 7. NANOBIOTECHNOLOGY AND NA
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7.2 Lipophilic nucleic acids as bui
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7.4 Nano- and microstructured surfa
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7.6 Proton microscopy - Quantitativ
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7.8 Investigation of the elastic pr
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POSTERS 8. BIOINFORMATICS
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8.2 Bio-Imaging platform LabImaging
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8.4 On the potential role of biomec
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8.6 Non-coding RNAs in Ciona intest
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8.8 Functional adaptations of dendr
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8.10 Multiple alignments of partial
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CENTER FOR BIOTECHNOLOGY AND BIOMED
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The following heads of research gro
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INDEX
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Dössel, Olaf 38 Drasdo, Dirk 205 E
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Lehmann, Jörg 65, 90 Lein, Sandro
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Spemann, Daniel 195 Stadler, Peter
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