I-Rel: a novel rei-related protein that inhibits NF-KB transcriptional ...

1 a3ATTXCX3XXX33CI,TXr)CtrrQ33aXCX3CAXXrCa3303^^ 90
91 cx^cjjxi3Cj^(3&cajTo:x7vccaujxxxjj^^ 180
M L R S G P A S G P S V 12
181 cccKri}xxxxi3XTjKTQaj^Gvc(jcaxxnxy3x:M^^ 270
1 3 P T G R A M P S R R V A R P P A A P E L G A L G S P D L S S 42
271 CTCiDX'iarx37iTia:MX¥G:ACA3via^ATia5GAT^ 360
4 3 L S L A V S R S T D E L E I I D E Y I K E N G F G L D G G Q 72
361 CG33XnD333CGA3333CroXA033GIOGIGIi:niirQ33^^ 450
7 3 P G P G E G L P R L V S R G A A S L S T V T L G P V A P P A 102
451 Aoxxixxx>criiaiix"!aTirc'TriirxrAciMJK?iaxrA3T}cx33xrxxTi^ 540
103 T P P P W G C P L G R L V S P A P G P G P O P H L V T T R O 132
541 CCCA?G::A3CI3:OXAia7JGr:GCIX'mCGAGiarA033XGCT01X^ 630
133 P K C R G M P F R Y E C E G R S A G S I I. G E S S T E A S K 162
63: AaX'IGaXX3CCATa3O.TrCa7XATIt7Tl7.?O3X'IG033^OGra^^ 720
163 T L P A I E L R D C G G L R E V E V T A C L V W K D W P H R 192
"/21 cjj:rMrxxjxy^jj:iry7Ki:xrAAfirACKxy€ix:]A(xrrA'i^^ 8io
153 V H P H 3 L. V G K D C T D G I C R V R L R P H V S P R H S F 222
811 /v^CMCX:'TOXJlA'K:r;^?ir;rGirMIAAGAAa:A'Al'IGatJX"irXT^^ 900
233 N K L G I 0 C V R K K E 1 E A A 1 E R K 1 0 L G 1 D P Y N A 252
931 aj:7iGCCIGAA3AACTJ\7r7«3WG170\CATrAATrjrOGIG,^3CX'K^^ 990
^:'3 G S L K N H 0 E V D M N V V R I C F 0 A S Y R D 0 0 G 0 M R 282
991 aJa^GG^^a.'iG^xl^\^:^7Gf\a:coGlc:A^G^CAAG^AAT3x:A:AAACA'J\lr^^^ 1080
283 R M D P V L S E P V Y D K K S T N T S E L R I C R I N K E S 312
: 081 (XixxjJiayiZv:yjv:iu.^Arr:xjypr'm:n'irx-'v:7iD:xy\C^ 1170
313 G P C T G G E E L Y L L C D K V 0 K E D I S V V F S R A S W 342
117] csAGJV033x:ni:'-AL'm:^nr7\(xxJxyKarKrN.yxxx:N.^j\Tv:xx 1260
343 E G R A P; F g C A D V H R 0 I A I V F K T P P Y E D r. E 1 V 372
1261 CTY3oxiGiGA;w?P.'jV\a?r3TTGG irj:Aix3X':a\Q0:3^ir33X!F.na:7o;rM33iATTCrj;Tnr7o?iAcciox"iaxi^ 1350
373 E P V T V N V F L 0 R L T D G V C S E P L P F T Y 1, P R D H 402
1351. (^k:yix^?A(xaxnuxk''yM:w^xx'yif^;Aaxxix:ATUJXx^(XJiuxviuxr^^ 1440
403 D 5 Y G V P; ;-: K A K R G M P D V L G E I. N S S D P H G I E S 432
1441 AV\03xa'yv\5^?v"jiAx\3Tjxx;AioGixaaACTia7iGax7A(XACQ3;7n:Axtrxxrpirx^ 153 o
433 K R R K K K p A 1 1, D H F L P N H G 3 G P F L P P S A L L P 462
1531 •yv3xncACTiu;\Tncia3:iALTjGiGKXxxxra-3xxia3\axT;(X'iTXXTxixtxt.i;\cci^ 1620
463 D P n F F S G T V S L P G L E P P G G p D L 1, D D G F A Y D 492
1621 CCI7vX03(;iX:ACAC'KTP37£CA10;TX?\CCiaT[aX033XrAa33(rACAC^ 1710
493 P T A P T L F T M L D L L P P A P P H A 3 A V V C S G G A G 522
1711 03JGIaX^KJ3G^^i\XXXXJ:3XXX^a^ACCACT•5^CACIOGACTaJ[ACC^taxa33^^ 1800
523 A V V G E 'P P G P E P L T L D 3 Y Q A P G P G D G G T A S L 552
1801 CTlG333A:XAAc:A7l.TTTXXKAATGATTti03GCGA33CaXCITiaXGXIIXC^^ 1890
553 V G 3 N M F P N R Y R F A A F G G G E P S P G P E A T * 582
1891 (G^'ITXrAG'a^Q3»:ACiaXnQ333G3GA3Gia¥>O3AOC03ia:M 1980
1981 71OGirAlIXriGT7AXTIlXiVj\TATirAGaTniX30GA3AAX:r^^ 2070
2071 AGii5^GiGaiw5Aa?wv\(XGACATOx:rccaxiai\ciAa7riG^ 216O
2161 ma5^TTrXI?AAAGATT!7IA;3AlAlQ33AG3ft333XOO\TICCIQGCCCiaxnr^ 2250
2251 aGIl(XllA^CJTC':'IC03AA,T?vVVGATXAGITITIQ\GO7ICAAAA?\/AAAAA?iAa7V\TIGC 2314
interaction of I-Rel with KB D N A was observed (see be­
low). In parallel reactions, performed with in vitro-trans-
lated RNA encoding the DNA-binding domain of the p50
and p65 subunits of NF-KB, strong association with a KB
probe was apparent as reported previously (Ruben et al.
1991).
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I-Rel inhibits NF-KB transcriptional activity
Figure 1. Nucleotide and predicted
amino acid sequence of the I-Rel cDNA.
The nucleotide sequence corresponding to
the beginning of the cDNA and the deduced
amino acid sequence of the longest
open reading frame is shown. The amino
acids corresponding to the rel homology
domain are underlined. Sequence data
have been submitted to the EMBL/Gen-
Bank data libraries under accession no.
M83221.
The precursor of p50, pl05, shows no binding activity
in vitro and must be truncated before demonstrating
binding activity (Ghosh et al. 1990; Kieran et al. 1990).
Therefore, I-Rel may also need to be processed in a sim­
ilar manner before it can bind DNA. To examine this
possibility, nucleotides encoding residues 1-425 of 1-Rel
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