1 DAVID WILLIAM CHRISTIANSON Roy and Diana Vagelos ...

Education:
DAVID WILLIAM CHRISTIANSON
Roy and Diana Vagelos Laboratories, Department of Chemistry
University of Pennsylvania, 231 South 34 th Street
Philadelphia, Pennsylvania 19104-6323
Phone: (215) 898-5714; email: chris@sas.upenn.edu
Ph.D. (chemistry), Harvard University, 1987.
M.A., University of Pennsylvania (honoris causa), 1993.
A.M., Harvard University, 1985.
A.B. (chemistry), magna cum laude, Harvard College, 1983.
Academic Appointments:
Fellow in Natural Sciences (Chemistry), Sidney Sussex College, University of Cambridge, 2006.
Roy and Diana Vagelos Professor in Chemistry and Chemical Biology, University of
Pennsylvania, 2003 - .
Edmund and Louise Kahn Professor in the Natural Sciences, University of Pennsylvania, 1999 -
2002.
Adjunct Professor, Wistar Institute, 1996 - .
Professor of Chemistry, University of Pennsylvania, 1996 - .
Associate Professor of Chemistry, University of Pennsylvania, 1993 - 1996.
Assistant Professor of Chemistry, University of Pennsylvania, 1988 - 1993.
Honors:
Citation from the Massachusetts State Senate for the Joseph P. Martin Award, 2009.
Visiting Professor (Underwood Fellowship), Department of Biochemistry,
University of Cambridge, 2006 - 2007.
Senior Fellow, American Asthma Foundation, 2006.
Fellow of the John Simon Guggenheim Memorial Foundation, 2006 - 2007.
Pfizer Award in Enzyme Chemistry, American Chemical Society, 1999.
Camille and Henry Dreyfus Teacher-Scholar Award, 1993 - 1994.
Faculty Dissertation Opponent, University of Lund, Sweden, 1992.
Alfred P. Sloan Research Fellow, 1992 - 1994.
Young Investigator Award, Office of Naval Research, 1989 - 1992.
Searle Scholar Award, Chicago Community Trust, 1989 - 1992.
AT&T Bell Laboratories Scholar, 1984 - 1987.
Oliver Wolcott Gibbs Prize in Chemistry, Harvard University, 1983.
American Institute of Chemists Student Awardee of Harvard College, 1983.
Blumberg Prize in Creative Science, Mather House, Harvard College, 1983.
Professional Experience:
Founder, Arginetix, Inc., 2008.
Postdoctoral Fellow, Harvard University, 1987 - 1988.
Resident Tutor in Chemistry, Mather House, Harvard College, 1983 - 1988.
Research Interests:
Structure and mechanism of metal-requiring enzymes; terpenoid biosynthesis and metabolism.
Structural and chemical biology of arginine metabolism in human disease.
1

DAVID WILLIAM CHRISTIANSON
BIBLIOGRAPHY
1. Shoham, G.; Christianson, D.W.; Bartsch, R.A.; Heo, G.S.; Olsher, U.; Lipscomb, W.N.
"Crystal and Molecular Structure of the Complex between sym-Dibenzo-14-crown-4-oxyacetate
and Li + , [C20H21O7] -. Li +. 7.5H2O". J. Am. Chem. Soc. 1984, 106, 1280-1285.
2. Christianson, D.W.; Lipscomb, W.N. "A Molecular Orbital Evaluation of Possible
Factors Affecting the Homolytic Activation of Coenzyme B12". J. Am. Chem. Soc. 1985, 107,
2682-2686.
3. Christianson, D.W.; Lipscomb, W.N. "Binding of a Possible Transition State Analogue
to the Active Site of Carboxypeptidase A". Proc. Natl. Acad. Sci. USA 1985, 82, 6840-6844.
4. Christianson, D.W.; Kuo, L.C.; Lipscomb, W.N. "Novel Structure of the Complex
between Carboxypeptidase A and a Ketonic Substrate Analogue". J. Am. Chem. Soc. 1985,
107, 8281-8283 (Communication to the Editor).
5. Christianson, D.W.; Lipscomb, W.N. "Structure of the Complex between an
Unexpectedly Hydrolyzed Phosphonamidate Inhibitor and Carboxypeptidase A". J. Am.
Chem. Soc. 1986, 108, 545-546 (Communication to the Editor).
6. Christianson, D.W.; Lipscomb, W.N. "Mechanistic Inferences from the Binding of
Ligands to the Active Site of Carboxypeptidase A". In Zinc Enzymes, Bertini, I.; Luchinat, C;
Maret, W.; Zeppezauer, M., Eds. Birkhauser: Boston, 1986; pp. 121-132.
7. Christianson, D.W.; Lipscomb, W.N. "The Complex between Carboxypeptidase A and a
Possible Transition State Analogue: Mechanistic Inferences from High Resolution X-ray
Structures of Enzyme-Inhibitor Complexes". J. Am. Chem. Soc. 1986, 108, 4998-5003.
8. Christianson, D.W.; Lipscomb, W.N. "X-ray Crystallographic Investigation of Substrate
Binding to Carboxypeptidase A at Subzero Temperature". Proc. Natl. Acad. Sci. USA 1986,
83, 7568-7572.
9. Christianson, D.W.; David, P.R.; Lipscomb, W.N. "Mechanism of Carboxypeptidase A:
Hydration of a Ketonic Substrate Analogue". Proc. Natl. Acad. Sci. USA 1987, 84, 1512-1515.
10. Christianson, D.W.; Lipscomb, W.N. "Carboxypeptidase A: Novel Enzyme-Substrate-
Product Complex". J. Am. Chem. Soc. 1987, 109, 5536-5538 (Communication to the Editor).
11. Shoham, G.; Christianson, D.W.; Oren, D.A. "Complex between Carboxypeptidase A
and a Hydrated Ketomethylene Substrate Analogue". Proc. Natl. Acad. Sci. USA 1988, 85,
684-688.
12. Christianson, D.W.; Lipscomb W.N. "Comparison of Carboxypeptidase A and
Thermolysin: Inhibition by Phosphonamidates". J. Am. Chem. Soc. 1988, 110, 5560-5565.
13. Lipscomb, W.N.; Christianson, D.W.; Shoham, G.; Oren, D.A. "Recent Studies of
Ligand Binding to Carboxypeptidase A". Biol. Chem. Hoppe-Seyler 1988, 369, 202-203.
2

DAVID WILLIAM CHRISTIANSON
14. Christianson, D.W.; Lipscomb, W.N. "Structural Aspects of the Tetrahedral Intermediate
in the Carboxypeptidase A Mechanism". In Molecular Structure: Chemical Reactivity and
Biological Reactivity; Stezowski, J.J.; Huang, J.-L.; Shao, M.-C., Eds. International Union of
Crystallography, Oxford University Press: Oxford, 1988; pp. 143-154.
15. Christianson, D.W.; Lipscomb, W.N. "Reaction Coordinate Approach to the Binding of
Ligands to Carboxypeptidase A". In 39. Mosbacher Colloquium, Protein Structure and
Protein Engineering; Winnaker, E.-L.; Huber, R., Eds. Springer-Verlag: New York, 1988; pp.
65-74.
16. Christianson, D.W.; Lipscomb, W.N. "Structural Aspects of Zinc Protease Mechanism".
In Mechanistic Principles of Enzyme Activity; Liebman, J.F.; Greenberg, A., Eds. VCH
Publishers, Inc.: New York, 1988; pp. 1-25.
17. Christianson, D.W.; Mangani, S.; Shoham, G.; Lipscomb, W.N. "Binding of
D-Phenylalanine and D-Tyrosine to Carboxypeptidase A". J. Biol. Chem. 1989, 264, 12849-
12853.
18. Christianson, D.W.; Lipscomb, W.N. "Carboxypeptidase A". Acc. Chem. Res. 1989,
22, 62-69.
19. Christianson, D.W.; Alexander, R.S. "Carboxylate-Histidine-Zinc Interactions in Protein
Structure and Function". J. Am. Chem. Soc. 1989, 111, 6412-6419.
20. Alexander, R.S.; Kanyo, Z.F.; Chirlian, L.E.; Christianson, D.W. "Stereochemistry of
Phosphate-Lewis Acid Interactions: Implications for Nucleic Acid Structure and Recognition".
J. Am. Chem. Soc. 1990, 112, 933-937.
21. Christianson, D.W.; Alexander, R.S. "Another Catalytic Triad?" Nature 1990, 346, 225.
22. Ippolito, J.A.; Alexander, R.S.; Christianson, D.W. "Hydrogen Bond Stereochemistry in
Protein Structure and Function". J. Mol. Biol. 1990, 215, 457-471.
23. Kanyo, Z.F.; Christianson, D.W. "Biological Recognition of Phosphate and Sulfate". J.
Biol. Chem. 1991, 226, 4264-4268.
24. Christianson, D.W.; Ippolito, J.A. "Structure-Function Relationships between the
Carbonic Anhydrases and the Zinc Proteases". In Carbonic Anhydrase: From Biochemistry
and Genetics to Physiology and Clinical Medicine; Botre, F.; Gros, G.; Storey, B.T., Eds.
VCH Publishers Inc.: New York, 1991; pp. 95-110.
25. Christianson, D.W. "Structural Biology of Zinc". Adv. Prot. Chem. 1991, 42, 281-355.
26. Alexander, R.S.; Nair, S.K.; Christianson, D.W. "Engineering the Hydrophobic Pocket
of Carbonic Anhydrase II". Biochemistry 1991, 30, 11064-11072.
27. Krebs, J.F.; Fierke, C.A.; Alexander, R.S.; Christianson, D.W. "Conformational Mobility
of His-64 in the Thr-200→Ser Mutant of Human Carbonic Anhydrase II". Biochemistry 1991,
30, 9153-9160.
3

DAVID WILLIAM CHRISTIANSON
28. Nair, S.K.; Calderone, T.L.; Christianson, D.W.; Fierke, C.A. "Altering the Mouth of a
Hydrophobic Pocket: Structure and Kinetics of Human Carbonic Anhydrase II Mutants at
Residue Val-121". J. Biol. Chem. 1991, 266, 17320-17325.
29. Nair, S.K.; Christianson D.W. "Unexpected pH-Dependent Conformation of His-64, the
Proton Shuttle of Human Carbonic Anhydrase II". J. Am. Chem. Soc. 1991, 113, 9455-9458.
30. Nair, S.K.; Christianson, D.W. "Structural Properties of Human Carbonic Anhydrase II
at pH 9.5". Biochem. Biophys. Res. Commun. 1991, 181, 579-584.
31. Kanyo, Z.F.; Chen, C.-Y.; Daghigh, F.; Ash, D.E.; Christianson, D.W. "Crystallization
and Oligomeric Structure of Rat Liver Arginase". J. Mol. Biol. 1992, 224, 1175-1177.
32. Wei, A.; Rubin, H.; Cooperman, B.S.; Schechter, N.; Christianson, D.W.
"Crystallization, Activity Assay, and Preliminary X-ray Diffraction Analysis of the Uncleaved
Form of the Serpin Antichymotrypsin". J. Mol. Biol. 1992, 226, 273-276.
33. Ippolito, J.A.; Christianson, D.W. "The Contribution of Halogen Atoms to Protein-
Ligand Affinity". Int. J. Biol. Macromol. 1992, 14, 193-197.
34. Cappalonga, A.M.; Alexander, R.S.; Christianson, D.W. "Structural Comparison of
Sulfodiimine and Sulfonamide Inhibitors in their Complexes with Zinc Enzymes". J. Biol.
Chem. 1992, 267, 19192-19197.
35. Alexander, R.S.; Kiefer, L.L.; Fierke, C.A.; Christianson, D.W. "Engineering the Zinc
Binding Site of Human Carbonic Anhydrase II: Structure of the His-94→Cys Apoenzyme in a
New Crystalline Form". Biochemistry 1993, 32, 1510-1518.
36. Nair, S.K.; Christianson, D.W. "Structural Consequences of Hydrophilic Amino Acid
Substitutions in the Hydrophobic Pocket of Human Carbonic Anhydrase II". Biochemistry
1993, 32, 4506-4514.
37. Nair, S.K.; Christianson, D.W. "Crystallographic Studies of Azide Binding to Human
Carbonic Anhydrase II". Eur. J. Biochem. 1993, 213, 507-515.
38. Lee, M.H.; Pankratz, H.S.; Wang, S.; Scott, R.A.; Johnson, M.K.; Ippolito, J.A.;
Christianson, D.W.; Hausinger, R.P. "Purification and Characterization of Klebsiella aerogenes
UreE Protein: a Nickel-Binding Protein that Functions in Urease Metallocenter Assembly".
Protein Science 1993, 2, 1042-1052.
39. Ippolito, J.A.; Christianson, D.W. "Structure of an Engineered His3Cys Zinc Binding
Site in Human Carbonic Anhydrase II". Biochemistry 1993, 32, 9901-9905.
40. Katz, D.S.; Christianson, D.W. "Modeling the Uncleaved Serpin Antichymotrypsin and
its Chymotrypsin Complex". Protein Eng. 1993, 6, 701-709.
41. Tweedy, N.B.; Nair, S.K.; Paterno, S.A.; Fierke, C.A.; Christianson, D.W. "Structure
and Energetics of a Non-proline cis-Peptidyl Linkage in a Proline-202→Alanine Carbonic
Anhydrase II Variant". Biochemistry 1993, 32, 10944-10949.
4

DAVID WILLIAM CHRISTIANSON
42. Katz, D.S.; Wei, A.; Zhong, Q.; Rubin, H.; Cooperman, B.S.; Christianson, D.W.
"Crystallization and Atomic Resolution X-Ray Diffraction Analysis of Antichymotrypsin
Variants". Biophys. Biochem. Res. Commun. 1993, 196, 752-757.
43. Kiefer, L.L.; Ippolito, J.A.; Fierke, C.A.; Christianson, D.W. "Redesigning the Zinc
Binding Site of Human Carbonic Anhydrase II: Structure of a His2Asp-Zn 2+ Metal Coordination
Polyhedron". J. Am. Chem. Soc. 1993, 115, 12581-12582 (Communication to the Editor).
44. Krebs, J.F.; Ippolito, J.A.; Christianson, D.W.; Fierke, C.A. "Structural and Functional
Importance of a Conserved Hydrogen Bond Network in Human Carbonic Anhydrase II". J.
Biol. Chem. 1993, 268, 27458-27466.
45. Smith, G.M.; Alexander, R.S.; Christianson, D.W.; McKeever, B.M.; Ponticello, G.S.;
Springer, J.P.; Randall, W.C.; Baldwin, J.J.; Habecker, C.N. "Positions of His-64 and a Bound
Water in Human Carbonic Anhydrase II upon Binding Three Structurally Related Inhibitors".
Protein Science 1994, 3, 118-125.
46. Christianson, D.W. "Three-Dimensional Structure of Uncleaved Antichymotrypsin:
Implications for Serpin Function". J. Cell. Biochem. 1994, 18D, 124 (Abstract).
47. Bunn, A.M.C.; Alexander, R.S.; Christianson, D.W. "Mapping Protein-Peptide
Interactions: Binding of Peptidylsulfonamide Inhibitors to Human Carbonic Anhydrase II". J.
Am. Chem. Soc. 1994, 116, 5063-5068.
48. Wei, A.; Rubin, H.; Cooperman, B.S.; Christianson, D.W. "Crystal Structure of an
Uncleaved Serpin Reveals the Conformation of an Inhibitory Reactive Loop". Nature Struct.
Biol. 1994, 1, 250-257.
49. Nair, S.K.; Ludwig, P.A.; Christianson, D.W. "Two-Site Binding of Phenol in the Active
Site of Human Carbonic Anhydrase II: Structural Implications for Substrate Association". J.
Am. Chem. Soc. 1994, 116, 3659-3660 (Communication to the Editor).
50. Jain, A.; Whitesides, G.M.; Alexander, R.S.; Christianson, D.W. "Identification of Two
Hydrophobic Patches in the Active-Site Cavity of Human Carbonic Anhydrase II by Solution-
Phase and Solid-State Studies and Their Use in the Development of Tight-Binding Inhibitors".
J. Med. Chem. 1994, 37, 2100-2105.
51. Katz, D.S.; White, S.P.; Huang, W.; Kumar, R.; Christianson, D.W. "Structure
Determination of Aquomet Porcine Hemoglobin at 2.8 Å Resolution". J. Mol. Biol. 1994, 244,
541-553.
52. Christianson, D.W.; Fierke, C.A. "Molecular Biosensors: The Roles of Molecular and
Structural Biology". Naval Research Reviews 1994, 46, 30-39 (cover story).
53. Ippolito, J.A.; Christianson, D.W. "Structural Consequences of Redesigning a Protein-
Zinc Binding Site". Biochemistry 1994, 33, 15241-15249.
54. Katz, D.S.; Christianson, D.W. "Structural Aspects of the Serpin Reaction Coordinate".
Perspectives in Drug Discovery and Design 1994, 2, 459-474.
5

DAVID WILLIAM CHRISTIANSON
55. Christianson, D.W.; Christianson, A.M.K. "Architecture and Design of Zinc Protein-
Ligand Complexes". In Chemical and Structural Approaches to Rational Drug Design;
Weiner, D.B.; Williams, W.B., Eds. Boca Raton: CRC Press Inc., 1995; pp. 215-236.
56. Ippolito, J.A.; Baird, T.T.; McGee, S.A.; Christianson, D.W.; Fierke, C.A. "Structure-
Assisted Redesign of a Protein-Zinc Binding Site with Femtomolar Affinity". Proc. Natl. Acad.
Sci. USA 1995, 92, 5017-5021.
57. Lesburg, C.A.; Christianson, D.W. "X-ray Crystallographic Studies of Engineered
Hydrogen Bond Networks in a Protein-Zinc Binding Site". J. Am. Chem. Soc. 1995, 117,
6838-6844.
58. Nair, S.K.; Krebs, J.F.; Christianson, D.W.; Fierke, C.A. "Structural Basis of Inhibitor
Affinity to Variants of Human Carbonic Anhydrase II". Biochemistry 1995, 34, 3981-3989.
59. Boriack, P.A.; Christianson, D.W.; Kingery-Wood, J.; Whitesides, G.M. "Secondary
Interactions Significantly Removed from the Sulfonamide Binding Pocket of Carbonic
Anhydrase II Influence Inhibitor Binding Constants". J. Med. Chem. 1995, 38, 2286-2291.
60. Boriack-Sjodin, P.A.; Heck, R.W.; Laipis, P.J.; Silverman, D.N.; Christianson, D.W.
"Structure Determination of Murine Mitochondrial Carbonic Anhydrase V at 2.45 Å Resolution:
Implications for Catalytic Proton Transfer and Inhibitor Design". Proc. Natl. Acad. Sci. USA
1995, 92, 10949-10953.
61. Ippolito, J.A.; Nair, S.K.; Alexander, R.S.; Kiefer, L.L.; Fierke, C.A.; Christianson, D.W.
"Structure of His-94→Asp Carbonic Anhydrase II in a New Crystalline Form Reveals a Partially-
Occupied Zinc Binding Site". Protein Eng. 1995, 8, 975-980.
62. Lesburg, C.A.; Lloyd, M.D.; Cane, D.E.; Christianson, D.W. "Crystallization and
Preliminary X-ray Diffraction Analysis of Recombinant Pentalenene Synthase". Protein
Science 1995, 4, 2436-2438.
63. Nair, S.K.; Elbaum, D.; Christianson, D.W. "Unexpected Binding Mode of the
Sulfonamide Fluorophore 5-Dimethylamino-1-Naphthalene Sulfonamide to Human Carbonic
Anhydrase II: Implications for the Development of a Zinc Biosensor". J. Biol. Chem. 1996,
271, 1003-1007.
64. Huang, C.-C.; Lesburg, C.A.; Kiefer, L.L.; Fierke, C.A.; Christianson, D.W. "Reversal
of the Hydrogen Bond to Zinc Ligand Histidine-119 Dramatically Diminishes Catalysis and
Enhances Metal Equilibration Kinetics in Carbonic Anhydrase II". Biochemistry 1996, 35,
3439-3446.
65. Lukacs, C.M.; Christianson, D.W. "Is the Binding of β-Amyloid Protein to
Antichymotrypsin in Alzheimer Plaques Mediated by a β-Strand Insertion?" Proteins: Struct.,
Funct. Genet. 1996, 25, 420-424.
66. Elbaum, D.E.; Nair, S.K.; Patchan, M.W.; Thompson, R.B.; Christianson, D.W.
"Structure-Based Design of a Sulfonamide Probe for Fluorescence Anisotropy Detection of Zinc
with a Carbonic Anhydrase-Based Biosensor". J. Am. Chem. Soc. 1996, 35, 8381-8387.
6

DAVID WILLIAM CHRISTIANSON
67. Christianson, D.W.; Fierke, C.A. "Carbonic Anhydrase: Evolution of the Zinc Binding
Site by Nature and by Design". Acc. Chem. Res. 1996, 29, 331-339.
68. Christianson, D.W. "Structural Aspects of Divergent Proton Translocation Trajectories
in Carbonic Anhydrases II and V". Rigaku Journal 1996, 13, 8-15.
69. Stavridi, E.; O'Malley, K.; Lukacs, C.M.; Moore, W.T.; Lambris, J.D.; Christianson,
D.W.; Rubin, H.; Cooperman, B.S. "Structural Change in α-Chymotrypsin Induced by
Complexation with α1-Antichymotrypsin as Seen by Enhanced Sensitivity to Proteolysis".
Biochemistry 1996, 35, 10608-10615.
70. Thompson, R.B.; Ge, Z.; Patchan, M.W.; Fierke, C.A.; McCall, K.A.; Elbaum, D.;
Christianson, D.W. "Determination of Multiple Analytes Using a Fiber Optic Biosensor Based
on Fluorescence Energy Transfer". Prog. Biomed. Optics (Proceedings of the SPIE Conference
on Ultrasensitive Biochemical Diagnostics, Cohn, G.E.; Soper, S.A.; Chen, C.H.W., Eds.) 1996,
2680, 47-56.
71. Lukacs, C.M.; Zhong, J.Q.; Rubin, H.; Cooperman, B.S.; Christianson, D.W. "Arginine
Substitutions in the Hinge Region of Antichymotrypsin Affect Serpin β-Sheet Rearrangement".
Nature Struct. Biol. 1996, 3, 888-893.
72. Heck, R.W.; Boriack-Sjodin, P.A.; Qian, M.; Tu, C.; Christianson, D.W.; Laipis, P.J.;
Silverman, D.N. "Structure-Based Design of an Intramolecular Proton Transfer Site in Murine
Carbonic Anhydrase V". Biochemistry 1996, 35, 11605-11611.
73. Kanyo, Z.F.; Scolnick, L.R.; Ash, D.E.; Christianson, D.W. "Structure of a Unique
Binuclear Manganese Cluster in Arginase". Nature 1996, 383, 554-557.
74. Stams, T.; Nair, S.K.; Okuyama, T.; Waheed, A.; Sly, W.S.; Christianson, D.W. "Crystal
Structure of the Secretory Form of Membrane-Associated Human Carbonic Anhydrase IV at 2.8
Å Resolution". Proc. Natl. Acad. Sci. USA 1996, 93, 13589-13594.
75. Scolnick, L.R.; Christianson, D.W. "X-ray Crystallographic Studies of Alanine-65
Variants of Carbonic Anhydrase II Reveal the Structural Basis of Compromised Proton Transfer
in Catalysis". Biochemistry 1996, 35, 16429-16434.
76. Christianson, D.W. "Structural Chemistry and Biology of Manganese Metalloenzymes".
Prog. Biophys. Mol. Biol. 1997, 67, 217-252.
77. Scolnick, L.R.; Kanyo, Z.F.; Cavalli, R.C.; Ash, D.E.; Christianson, D.W. "Altering the
Binuclear Cluster of Arginase Diminishes Thermostability and Catalytic Function".
Biochemistry 1997, 36, 10558-10565.
78. Christianson, D.W. "Structure-Function Relationships in the Zinc and Manganese
Metalloenzymes of Mammalian Nitrogen Metabolism". J. Inorg. Biochem. 1997, 67, 216
(Abstract).
79. Scolnick, L.R.; Clements, A.M.; Liao, J.; Crenshaw, L.; Hellberg, M.; May, J.; Dean,
T.R.; Christianson, D.W. "Novel Binding Mode of Hydroxamate Inhibitors to Human Carbonic
Anhydrase II". J. Am. Chem. Soc. 1997, 119, 850-851 (Communication to the Editor).
7

DAVID WILLIAM CHRISTIANSON
80. Lesburg, C.A.; Huang, C.-C.; Christianson, D.W.; Fierke, C.A. "Histidine→ Carboxamide
Ligand Substitutions in the Zinc Binding Site of Carbonic Anhydrase II Alter Metal
Coordination Geometry but Retain Catalytic Activity". Biochemistry 1997, 36, 15780-15791.
81. Baggio, R.; Elbaum, D.; Kanyo, Z.F.; Carroll, P.J.; Cavalli, R.C.; Ash, D.E.;
Christianson, D.W. "Inhibition of Mn 2+ 2-Arginase by Borate Leads to the Design of a Transition
State Analogue Inhibitor, 2(S)-Amino-6-boronohexanoic Acid". J. Am. Chem. Soc. 1997, 119,
8107-8108 (Communication to the Editor).
82. Lesburg, C.A.; Zhai, G.; Cane, D.E.; Christianson, D.W. "Crystal Structure of
Pentalenene Synthase: Mechanistic Insights on Terpenoid Cyclization Reactions in Biology".
Science 1997, 277, 1820-1824.
83. Lukacs, C.M.; Rubin, H.; Christianson, D.W. "Engineering an Anion-Binding Cavity in
Antichymotrypsin Modulates the 'Spring-Loaded Serpin-Protease Interaction". Biochemistry
1998, 37, 3297-3304.
84. Stams, T.; Chen, Y.; Boriack-Sjodin, P.A.; Hurt, J.D.; Liao, J.; May, J.A.; Dean, T.;
Laipis, P.; Silverman, D.N.; Christianson, D.W. "Structures of Murine Carbonic Anhydrase IV
and Human Carbonic Anhydrase II Complexed with Brinzolamide: Molecular Basis of Isozyme-
Drug Discrimination". Protein Science 1998, 7, 556-563.
85. Stams, T.; Niranjanakumari, S.; Fierke, C.A.; Christianson, D.W. "Ribonuclease P
Protein Structure: Evolutionary Origins in the Translational Apparatus". Science 1998, 280,
752-755.
86. Ash, D.E.; Scolnick, L.R.; Kanyo, Z.F.; Vockley, J.G.; Cederbaum, S.D.; Christianson,
D.W. "Molecular Basis of Hyperargininemia: Structure-Function Consequences of Mutations in
Human Liver Arginase". Mol. Genet. Metabolism 1998, 64, 243-249 (cover article).
87. Boriack-Sjodin, P.A.; Zeitlin, S.; Chen, H.-H.; Crenshaw, L.; Gross, S.; Dantanarayana,
A.; Delgado, P.; May, J.A.; Dean, T.; Christianson, D.W. "Structural Analysis of Inhibitor
Binding to Human Carbonic Anhydrase II". Protein Science 1998, 7, 2483-2489.
88. Lesburg, C.A.; Caruthers, J.M.; Paschall, C.M.; Christianson, D.W. “Managing and
Manipulating Carbocations in Biology: Terpenoid Cyclase Structure and Mechanism”. Curr.
Op. Struct. Biol. 1998, 8, 695-703.
89. Niranjanakumari, S.; Stams, T.; Crary, S.M.; Christianson, D.W.; Fierke, C.A. “Protein
Component of the Ribozyme Ribonuclease P Alters Substrate Recognition by Directly
Contacting pre-tRNA”. Proc. Natl. Acad. Sci. USA 1998, 95, 15212-15217.
90. Christianson, D.W.; Cox J.D. “Catalysis by Metal-Activated Hydroxide in Zinc and
Manganese Metalloenzymes”. Annu. Rev. Biochem. 1999, 68, 33-57.
91. Paschall, C.M.; Hasserodt, J.; Jones, T.; Janda, K.D.; Lerner, R.A.; Christianson, D.W.
"Konvergenz der Mechanismen eines katalytischen Antikörpers und einer Terpen-Cyclase: eine
durch Carbokation-π-Elektronen-Wechselwirkung gesteuerte Polyencyclisierung". Angew.
Chem. 1999, 111, 1859-1864. "Convergence of Catalytic Antibody and Terpene Cyclase
Mechanisms: Polyene Cyclization Directed by Carbocation-π Interactions". Angew. Chem.
Intl. Ed. 1999, 38, 1743-1747 (cover article).
8

DAVID WILLIAM CHRISTIANSON
92. Baggio, R.; Emig, F.A.; Christianson, D.W.; Ash, D.E.; Chakder, S.; Rattan, S.
“Biochemical and Functional Profile of a Newly Developed Potent and Isozyme-Selective
Arginase Inhibitor”. J. Pharm. Exp. Ther. 1999, 290, 1409-1416.
93. Argiriadi, M.A.; Morriseau, C.; Hammock, B.D.; Christianson, D.W. "Detoxification of
Environmental Mutagens and Carcinogens: Structure, Mechanism, and Evolution of Liver
Epoxide Hydrolase". Proc. Natl. Acad. Sci. USA 1999, 96, 10637-10642.
94. Ash, D.E.; Cox, J.D.; Christianson, D.W. "Arginase: A Binuclear Manganese
Metalloenzyme". In Manganese and Its Role in Biological Processes, Vol. 37 of Metal Ions
in Biological Systems, Sigel, A., Sigel, H., Eds., 1999; New York: M. Dekker, 407-428.
95. Cox, J.D.; Kim, N.N.; Traish, A.M.; Christianson, D.W. "Arginase-Boronic Acid
Complex Highlights a Physiological Role in Erectile Function". Nature Struct. Biol. 1999, 6,
1043-1047.
96. Baggio, R.; Cox, J.D.; Harper, S.L.; Speicher, D.W.; Christianson, D.W. "A New
Chromophoric Assay for Arginase Activity". Anal. Biochem. 1999, 276, 251-253.
97. Argiriadi, M.A.; Morisseau, C.; Goodrow, M.H.; Dowdy, D.L.; Hammock, B.D.;
Christianson, D.W. "Binding of Alkylurea Inhibitors to Epoxide Hydrolase Implicates Active
Site Tyrosines in Substrate Activation". J. Biol. Chem. 2000, 275, 15265-15270.
98. Doyon, J.B.; Hansen, E.A.M.; Kim, C.-Y.; Chang, J.S.; Christianson, D.W.; Madder,
R.D.; Voet, J.G.; Baird, T.A.; Fierke, C.A.; Jain, A. "Linear Free Energy Relationships
Implicate Three Modes of Binding for Fluroaromatic Inhibitors to a Mutant of Carbonic
Anhydrase II". Org. Lett. 2000, 2, 1189-1192.
99. Stams, T.; Christianson, D.W. "X-ray Crystallographic Studies of Mammalian Carbonic
Anhydrase Isozymes". In The Carbonic Anhydrases: New Horizons, Chegwidden, W.R.,
Carter, N.D., & Edwards, Y.H., Eds., 2000. Basel: Birkhäuser, pp. 159-174 (book cover).
100. Hunt, J.A.; Lesburg, C.A.; Christianson, D.W.; Thompson, R.B.; Fierke, C.A. "Active-
Site Engineering of Carbonic Anhydrase and Its Application to Biosensors". In The Carbonic
Anhydrases: New Horizons, Chegwidden, W.R., Carter, N.D., & Edwards, Y.H., Eds., 2000.
Basel: Birkhäuser, pp. 221-240.
101. Yamada, T.; Morisseau, C.; Maxwell, J.E.; Argiriadi, M.A.; Christianson, D.W.;
Hammock, B.D. "Biochemical Evidence for the Involvement of Tyrosine in Epoxide Activation
During the Catalytic Cycle of Epoxide Hydrolase. J. Biol. Chem. 2000, 275, 23082-23088.
102. Caruthers, J.M.; Kang, I.; Rynkiewicz, M.J.; Cane, D.E.; Christianson, D.W. "Crystal
Structure Determination of Aristolochene Synthase from the Blue Cheese Mold, Penicillium
roqueforti". J. Biol. Chem. 2000, 275, 25533-25539.
103. Cox, J.D.; Hunt, J.A.; Compher, K.M.; Fierke, C.A.; Christianson, D.W. "Structural
Influence of Hydrophobic Core Residues on Metal Binding and Specificity in Carbonic
Anhydrase II". Biochemistry 2000, 39, 13687-13694.
9

DAVID WILLIAM CHRISTIANSON
104. Kim, C.-Y.; Chang, J.S.; Doyon, J.B.; Baird, T.T.; Fierke, C.A.; Jain, A.; Christianson,
D.W. "Contribution of Fluorine to Protein-Ligand Affinity in the Binding of Fluoroaromatic
Inhibitors to Carbonic Anhydrase II". J. Am. Chem. Soc. 2000, 122, 12125-12134.
105. Lavulo, L.T.; Sossong, T.M.; Brigham-Burke, M.R.; Doyle, M.L.; Cox, J.D.;
Christianson, D.W.; Ash, D.E. "Subunit-Subunit Interactions in Trimeric Arginase: Generation
of Active Monomers by Mutation of a Single Amino Acid". J. Biol. Chem. 2001, 276, 14242-
14248.
106. Cox, J.D.; Christianson, D.W. "Arginase: Structure, Mechanism, and Role in Erectile
Function". In Transactions of the American Crystallographic Association, Ohlendorf, D.,
Ed., 2001. Buffalo: American Crystallographic Association, pp. 85-101.
107. Kim, N.N.; Cox, J.D.; Baggio, R.F.; Emig, F.A.; Mistry, S.K.; Harper, S.L.; Speicher,
D.W.; Morris, S.M.; Ash, D.E.; Traish, A.; Christianson, D.W. "Probing Erectile Function: S-
(2-Boronoethyl)-L-Cysteine Binds to Arginase as a Transition State Analogue and Enhances
Smooth Muscle Relaxation in Human Penile Corpus Cavernosum". Biochemistry 2001, 40,
2678-2688.
108. Cox, J.D.; Cama, E.; Colleluori, D.M.; Pethe, S.; Boucher, J.-L.; Mansuy, D.; Ash, D.E.;
Christianson, D.W. "Mechanistic and Metabolic Inferences from the Binding of Substrate
Analogues and Products to Arginase". Biochemistry 2001, 40, 2689-2701.
109. Whittington, D.A.; Waheed, A.; Ulmasov, B.; Shah, G.N.; Grubb, J.H.; Sly, W.S.;
Christianson, D.W. "Crystal Structure of the Dimeric Extracellular Domain of Human Carbonic
Anhydrase XII, a Bitopic Membrane Protein Overexpressed in Certain Cancer Tumor Cells".
Proc. Natl. Acad. Sci. USA 2001, 98, 9545-9550.
110. Kim, C.-Y.; Chandra, P.P.; Jain, A.; Christianson, D.W. "Fluoroaromatic-Fluoroaromatic
Interactions between Inhibitors Bound in the Crystal Lattice of Human Carbonic Anhydrase II".
J. Am. Chem. Soc. 2001, 123, 9620-9627.
111. Rynkiewicz, M.J.; Cane, D.E.; Christianson, D.W. "Structure of Trichodiene Synthase
from Fusarium sporotrichioides Provides Mechanistic Inferences on the Terpene Cyclization
Cascade". Proc. Natl. Acad. Sci. USA 2001, 98, 13543-13548.
112. Rynkiewicz, M.J.; Cane, D.E.; Christianson, D.W. "X-ray Crystal Structures of D100E
Trichodiene Synthase and Its Diphosphate Complex Reveal Basis for Terpene Product
Diversity". Biochemistry 2002, 41, 1732-1741.
113. Madder, R.D.; Kim, C.-Y.; Chandra, P.P.; Doyon, J.B.; Baird, T.T.; Fierke, C.A.;
Christianson, D.W.; Voet, J.G.; Jain, A. "Twisted Amides Inferred from QSAR Analysis of
Hydrophobicity and Electronic Effects on the Affinity of Fluoroaromatic Inhibitors of Carbonic
Anhydrase". J. Org. Chem. 2002, 67, 582-584.
114. Grzybowski, B.A.; Ishchenko, A.V.; Kim, C.-Y.; Topalov, G.; Chapman, R.;
Christianson, D.W.; Whitesides, G.M.; Shakhnovich, E.I. "Combinatorial Computational
Method Gives New Picomolar Ligands for a Known Enzyme". Proc. Natl. Acad. Sci. USA
2002, 99, 1270-1273.
10

DAVID WILLIAM CHRISTIANSON
115. Kim, C.-Y.; Whittington, D.A.; Chang, J. S.; Liao, J.; May, J.A.; Christianson, D.W.
"Structural Aspects of Isozyme Selectivity in the Binding of Inhibitors to Carbonic Anhydrases
II and IV". J. Med. Chem. 2002, 45, 888-893.
116. Jude, K.M.; Wright, S.K.; Tu, C.; Silverman, D.N.; Viola, R.E.; Christianson, D.W.
"Crystal Structure of F65A/Y131C-Methylimidazole Carbonic Anhydrase V Reveals
Architectural Features of an Engineered Proton Shuttle ". Biochemistry 2002, 41, 2485-2491.
117. Seemann, M.; Zhai, G.; de Kraker, J.-W.; Paschall, C.M.; Christianson, D.W.; Cane, D.E.
"Pentalenene Synthase. Analysis of Active Site Residues by Site-Directed Mutagenesis".
J. Am. Chem. Soc. 2002, 124, 7681-7689.
118. Whittington, D.A.; Wise, M.L.; Urbansky, M.; Coates, R.M.; Croteau, R.B.;
Christianson, D.W. "Bornyl Diphosphate Synthase: Structure and Strategy for Carbocation
Manipulation by a Terpenoid Cyclase". Proc. Natl. Acad. Sci. USA 2002, 99, 15375-15380.
119. Whittington, D.A.; Rusche, K.M.; Shin, H.; Fierke, C.A.; Christianson, D.W. "Crystal
Structure of LpxC, a Zinc-Dependent Deacetylase Essential for Endotoxin Biosynthesis". Proc.
Natl. Acad. Sci. USA 2003, 100, 8146-8150.
120. Cama, E.; Emig, F.A.; Ash, D.E.; Christianson, D.W. "Structural and Functional
Importance of First-Shell Metal Ligands in the Binuclear Manganese Cluster of Arginase I".
Biochemistry 2003, 42, 7748-7758.
121. Cama, E.; Colleluori, D.M.; Emig, F.A.; Shin, H.; Kim, S.W.; Kim, N.N.; Traish, A.;
Ash, D.E.; Christianson, D.W. "Human Arginase II: Crystal Structure and Physiological Role in
Male and Female Sexual Arousal". Biochemistry 2003, 42, 8445-8451.
122. Xu, L.; Hilliard, B.; Carmody, R.J.; Tsabary, G.; Shin, H.; Christianson, D.W.; Chen, Y.
"Arginase and Autoimmune Inflammation in the Central Nervous System". Immunology 2003,
110, 141-148.
123. Cama, E.; Shin, H.; Christianson, D.W. "Design of Amino Acid Sulfonamides as
Transition-State Analogue Inhibitors of Arginase". J. Am. Chem. Soc. 2003, 125, 13052-
13057.
124. Whittington, D.A.; Grubb, J.H.; Waheed, A.; Shah, G.N.; Sly, W.S.; Christianson, D.W.
"Expression, Assay, and Structure of the Extracellular Domain of Murine Carbonic Anhydrase
XIV: Implications for Selective Inhibition of Membrane-Associated Isozymes". J. Biol. Chem.
2004, 279, 7223-7228.
125. Gomez, G.A.; Morisseau, C.; Hammock, B.D.; Christianson, D.W. "Structure of Human
Epoxide Hydrolase Reveals Mechanistic Inferences on Bifunctional Catalysis in Epoxide and
Phosphate Ester Hydrolysis". Biochemistry 2004, 43, 4716-4723.
126. Cama, E.; Pethe, S.; Boucher, J.-L.; Han, S.; Emig, F.A.; Ash, D.E.; Viola, R.E.; Mansuy,
D.; Christianson, D.W. "Inhibitor Coordination Interactions in the Binuclear Manganese Cluster
of Arginase". Biochemistry 2004, 43, 8987-8999.
11

DAVID WILLIAM CHRISTIANSON
127. Shin, H.; Cama, E.; Christianson, D.W. "Design of Amino Acid Aldehydes as
Transition-State Analogue Inhibitors of Arginase". J. Am. Chem. Soc. 2004, 126, 10278-
10284.
128. Kim, N.N.; Christianson, D.W.; Traish, A.M. "Role of Arginase in the Male and Female
Sexual Arousal Response". J. Nutrition 2004, 134, 2873-2879.
129. Hernick, M.; Gennadios, H.A.; Whittington, D.A.; Rusche, K.M.; Christianson, D.W.;
Fierke, C.A. "UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine Deacetylase Functions
through a Unique General Acid-Base Catalyst Pair Mechanism". J. Biol. Chem. 2005, 280,
16969-16978.
130. Vedula, L.S.; Rynkiewicz, M.J.; Pyun, H.-J.; Coates, R.M.; Cane, D.E.; Christianson,
D.W. "Molecular Recognition of the Substrate Diphosphate Group Governs Product Diversity in
Trichodiene Synthase Mutants". Biochemistry 2005, 44, 6153-6163.
131. Christianson, D.W. "Arginase: Structure, Mechanism, and Physiological Role in Male
and Female Sexual Arousal". Acc. Chem. Res. 2005, 38, 191-201 (invited review).
132. Di Costanzo, L.; Sabio, G.; Mora, A.; Rodriguez, P.C.; Ochoa, A.C.; Centeno, F.;
Christianson, D.W. "Crystal Structure of Human Arginase I at 1.29-Å Resolution and
Exploration of Inhibition in the Immune Response". Proc. Natl. Acad. Sci. USA 2005, 102,
13058-13063.
133. Vedula, L.S.; Cane, D.E.; Christianson, D.W. "Role of Arginine-304 in the Diphosphate-
Triggered Active Site Closure Mechanism of Trichodiene Synthase". Biochemistry 2005, 44,
12719-12727.
134. Colleluori, D.M.; Reczkowski, R.S.; Emig, F.A.; Cama, E.; Cox, J.D.; Scolnick, L.R.;
Compher, K.; Jude, K.; Han, S.; Viola, R.E.; Christianson, D.W.; Ash, D.E. "Probing the Role
of the Hyper-Reactive Histidine Residue of Arginase". Arch. Biochem. Biophys. 2005, 444,
15-26.
135. Gomez, G.A.; Morisseau, C.; Hammock, B.D.; Christianson, D.W. "Human Soluble
Epoxide Hydrolase: Structural Basis of Inhibition by 4-(3-Cyclohexylureido)-Carboxylic
Acids". Protein Science 2006, 15, 58-64.
136. Christianson, D.W. "Structural Biology and Chemistry of the Terpenoid Cyclases".
Chem. Rev. 2006, 106, 3412-3442 (invited review; cover article).
137. Jude, K.M.; Banerjee, A.L.; Haldar, M.K.; Manokaran, S.; Roy, B.; Mallik, S.;
Srivastava, D.K.; Christianson, D.W. "Ultrahigh Resolution Crystal Structures of Human
Carbonic Anhydrases I and II Complexed with 'Two-Prong' Inhibitors Reveal the Molecular
Basis of High Affinity". J. Am. Chem. Soc. 2006, 128, 3011-3018.
138. Gennadios, H.A.; Whittington, D.A.; Li, X.; Fierke, C.A.; Christianson, D.W.
"Mechanistic Inferences from the Binding of Ligands to LpxC, a Metal-Dependent Deacetylase".
Biochemistry 2006, 45, 7940-7948.
139. Christianson, D.W. "Five Golden Rings". Science 2006, 311, 1382-1383 (Perspective).
12

DAVID WILLIAM CHRISTIANSON
140. Di Costanzo, L.; Flores, L.V.; Christianson, D.W. "Stereochemistry of Guanidine-Metal
Interactions: Implications for L-Arginine-Metal Interactions in Protein Structure and Function.
Proteins: Struct., Funct., Bioinf. 2006, 65, 637-642.
141. Gennadios, H.A.; Christianson, D.W. "Binding of Uridine 5’-Diphosphate in the "Basic
Patch" of the Zinc Deacetylase LpxC and Implications for Substrate Binding". Biochemistry
2006, 45, 15216-15223.
142. De Vivo, M.; Ensing, B.; Dal Peraro, M.; Gomez, G.A.; Christianson, D.W.; Klein, M.L.
"Proton Shuttles and Phosphatase Activity in Soluble Epoxide Hydrolase". J. Am. Chem. Soc.
2007, 129, 387-394.
143. Di Costanzo, L.; Gomez, G.A.; Christianson, D.W. "Crystal Structure of Lactaldehyde
Dehydrogenase from Escherichia coli and Inferences Regarding Substrate and Cofactor
Specificity". J. Mol. Biol. 2007, 366, 481-493.
144. Krishnamurthy, V.M.; Bohall, B.R.; Kim, C.-Y.; Moustakas, D.T.; Christianson, D.W.;
Whitesides, G.M. "Thermodynamic Parameters for the Association of Fluorinated
Benzenesulfonamides with Bovine Carbonic Anhydrase II". Chem. Asian J. 2007, 2, 94-105.
145. Shishova, E.K.; Di Costanzo, L.; Cane, D.E.; Christianson, D.W. "X-ray Crystal
Structure of Aristolochene Synthase from Aspergillus terreus and the Evolution of Templates for
the Cyclization of Farnesyl Diphosphate". Biochemistry 2007, 46, 1941-1951.
146. Shin, H.; Gennadios, H.A.; Whittington, D.A.; Christianson, D.W. "Amphipathic
Benzoic Acid Derivatives: Synthesis and Binding in the Hydrophobic Tunnel of the Zinc
Deacetylase LpxC". Bioorg. Med. Chem. 2007, 15, 2617-2623.
147. Christianson, D.W. "Roots of Biosynthetic Diversity". Science 2007, 316, 60-61
(Perspective).
148. Srivastava, D.K.; Jude, K.M.; Banerjee, A.L.; Haldar, M.; Manokaran, S.; Kooren, J.;
Mallik, S.; Christianson, D.W. "Structural Analysis of Charge Discrimination in the Binding of
Inhibitors to Human Carbonic Anhydrases I and II". J. Am. Chem. Soc. 2007, 129, 5528-5537.
149. Di Costanzo, L.; Pique, M.E.; Christianson, D.W. "Crystal Structure of Human Arginase
I Complexed with Thiosemicarbazide Reveals an Unusual Thiocarbonyl µ-Sulfide Ligand in the
Binuclear Manganese Cluster". J. Am. Chem. Soc. 2007, 129, 6388-6389 (Communication to
the Editor).
150. Di Costanzo, L.; Moulin, M.; Haertlein, M.; Meilleur, F.; Christianson, D.W.
"Expression, Purification, Assay, and Crystal Structure of Perdeuterated Human Arginase I".
Arch. Biochem. Biophys. 2007, 465, 82-89.
151. Vedula, L.S.; Zhao, Y.; Coates, R.M.; Koyama, T.; Cane, D.E.; Christianson, D.W.
"Exploring Biosynthetic Diversity with Trichodiene Synthase". Arch. Biochem. Biophys.
2007, 466, 260-266.
13

DAVID WILLIAM CHRISTIANSON
152. Santhanam, L.; Lim, H.K.; Lim, H.K.; Miriel, V.; Brown, T.; Patel, M.; Balanson, S.;
Ryoo, S.; Anderson, M.; Irani, K.; Khanday, F.; Di Costanzo, L.; Nyhan, D.; Hare, J.;
Christianson, D.W.; Rivers, R.; Shoukas, A.; Berkowitz, D.E. "iNOS-Dependent S-Nitrosylation
and Activation of Arginase I Contributes to Age Related Endothelial Dysfunction". Circulation
Res. 2007, 101, 692-702.
153. Vedula, L.S.; Jiang, J.; Zakharian, T.; Cane, D.E.; Christianson, D.W. "Structural and
Mechanistic Analysis of Trichodiene Synthase using Site-Directed Mutagenesis: Probing the
Catalytic Function of Tyrosine-295 and the Asparagine-225/Serine-229/Glutamate-233–Mg 2+
B
Motif". Arch. Biochem. Biophys. 2008, 469, 184-194.
154. Christianson, D.W. "Unearthing the Roots of the Terpenome". Curr. Op. Chem. Biol.
2008, 12, 141-150 (invited review).
155. Ryoo, S.; Gupta, G.; Benjo, A.; Lim, H.K.; Camera, A.; Sikka, G.; Lim, H.K.; Sohi, J.;
Santhanam, L.; Soucy, K.; Tuday, E.; Baraban, E.; Ilies, M.; Gerstenblith, G.; Nyhan, D.;
Shoukas, A.; Christianson, D.W.; Alp, N.J.; Champion, H.C.; Huso, D.; Berkowitz, D.E.
"Endothelial Arginase II: A Novel Target for the Treatment of Atherosclerosis". Circulation
Res. 2008, 102, 923-932.
156. Aaron, J.A.; Chambers, J.M.; Jude, K.M.; Di Costanzo, L.; Dmochowski, I.J.;
Christianson, D.W. "Structure of a 129 Xe-Cryptophane Biosensor Complexed with Human
Carbonic Anhydrase II". J. Am. Chem. Soc. 2008, 130, 6942-6943.
157. Dowling, D.P.; Di Costanzo, L.; Gennadios, H.A.; Christianson, D.W. "Evolution of the
Arginase Fold and Functional Diversity". Cell. Mol. Life Sci. 2008, 65, 2039-2055.
158. Shishova, E.Y.; Yu, F.; Miller, D.J.; Faraldos, J.A.; Zhao, Y.; Coates, R.M.; Allemann,
R.K.; Cane, D.E.; Christianson, D.W. "X-ray Crystallographic Studies of Substrate Binding to
Aristolochene Synthase Suggest a Metal Binding Sequence for Catalysis". J. Biol. Chem. 2008,
283, 15431-15439.
159. Di Costanzo, L.; Drury, J.E.; Penning, T.; Christianson, D.W. "Crystal Structure of
Human Δ 4 -3-Ketosteroid 5β-Reductase (AKR1D1) and Implications for Substrate Binding and
Catalysis". J. Biol. Chem. 2008, 283, 16830-16839 (Paper of the Week, 13 June 2008).
160. Santhanam, L.; Christianson, D.W.; Nyhan, D.; Berkowitz, D. "Arginase and Vascular
Aging". J. Appl. Physiol. 2008, 105, 1632-1642.
161. Zakharian, T.Y.; Di Costanzo, L.; Christianson, D.W. "Synthesis of (2S)-2-Amino-7,8-
Epoxyoctanoic Acid and Structure of its Metal-Bridging Complex with Human Arginase I".
Org. Biomol. Chem. 2008, 6, 3240-3243.
162. Butts, C.A.; Swift, J.; Kang, S.-G.; Di Costanzo, L.; Christianson, D.W.; Saven, J.G.;
Dmochowski, I.J. "Directing Noble Metal Ion Chemistry within a Designed Ferritin Protein".
Biochemistry 2008, 47, 12729-12739.
163. Dowling, D.P.; Gantt, S.L.; Gattis, S.G.; Fierke, C.A.; Christianson, D.W. "Structural
Studies of Human Histone Deacetylase 8 and its Site-Specific Variants Complexed with
Substrate and Inhibitors". Biochemistry 2008, 47, 13554-13563.
14

DAVID WILLIAM CHRISTIANSON
164. Zakharian, T.Y.; Di Costanzo, L.; Christianson, D.W. "(S)-2-Amino-6-Nitrohexanoic
Acid Binds to Human Arginase I through Multiple Nitro-Metal Coordination Interactions in the
Binuclear Manganese Cluster". J. Am. Chem. Soc. 2008, 130, 17254-17255 (Communication
to the Editor).
165. Chambers, J.M.; Hill, P.A.; Aaron, J.A.; Han, Z.; Christianson, D.W.; Kuzma, N.N.;
Dmochowski, I.J. "Cryptophane Xenon-129 Nuclear Magnetic Resonance Biosensors Targeting
Human Carbonic Anhydrase". J. Am. Chem. Soc. 2009, 131, 563-569.
166. Shishova, E.Y.; Di Costanzo, L.; Emig, F.A; Ash, D.E.; Christianson, D.W. "Probing the
Specificity Determinants of Amino Acid Recognition by Arginase". Biochemistry 2009, 48,
121-131.
167. Di Costanzo, L.; Drury, J.E.; Christianson, D.W.; Penning, T.M. "Structure and Catalytic
Mechanism of Human Steroid 5β-Reductase (AKR1D1). Mol. Cell Endocrinol. 2009, 301,
191-198.
168. Di Costanzo, L.; Penning, T.M.; Christianson, D.W. "Aldo-Keto Reductases in which
the Conserved Catalytic Histidine is Substituted". Chem.-Biol. Interactions 2009, 178, 127-
133.
169. Gennadios, H.A.; Gonzalez, V.; Di Costanzo, L.; Li, A.; Yu, F.; Miller, D.J.; Allemann,
R.K.; Christianson, D.W. "Crystal Structure of (+)-δ-Cadinene Synthase from Gossypium
arboreum and Evolutionary Divergence of Metal Binding Motifs for Catalysis". Biochemistry
2009, 48, 6175-6183.
170. Drury, J.E.; Di Costanzo, L.; Penning, T.M.; Christianson, D.W. "Inhibition of Human
Steroid 5β-Reductase (AKR1D1) by Finasteride and Structure of the Enzyme-Inhibitor
Complex". J. Biol. Chem. 2009, 284, 19786-19790 (Accelerated Publication).
171. Kim, J.H.; Bugaj, L.; Oh, Y.J.; Bivalacqua, T.; Ryoo, S.; Soucy, K.G.; Santhanam, L.;
Webb, A.; Camara, A.; Sikka, G.; Nyhan, D.; Shoukas, A.; Ilies, M.; Christianson, D.W.;
Champion, H.C.; Berkowitz, D.E. "Arginase Inhibition Restores NOS Coupling and Reverses
Endothelial Dysfunction and Vascular Stiffness in Old Rats". J. Appl. Physiol. 2009, 107,
1249-1257.
172. Aaron, J.A.; Lin, X.; Cane, D.E.; Christianson, D.W. "Structure of Epi-Isozizaene
Synthase from Streptomyces coelicolor A3(2), a Platform for New Terpenoid Cyclization
Templates". Biochemistry 2010, 49, 1787-1797.
173. Di Costanzo, L.; Ilies, M.; Thorn, K.J.; Christianson, D.W. "Inhibition of Human
Arginase I by Substrate and Product Analogues". Arch. Biochem. Biophys. 2010, 496, 101-
108.
174. Ilies, M.; Di Costanzo, L.; North, M.L.; Scott, J.A.; Christianson, D.W. "2-Amino-
Imidazole Amino Acids as Inhibitors of the Binuclear Manganese Metalloenzyme Human
Arginase I". J. Med. Chem. 2010, 53, 4266-4276.
175. Zakharian, T.Y.; Christianson, D.W. "Design and Synthesis of C 60-Benzenesulfonamide
Conjugates". Tetrahedron Lett. 2010, 51, 3645-3648.
15

DAVID WILLIAM CHRISTIANSON
176. Herbert, De'B.R.; Orekov, T.; Rolonson, A.; Ilies, M.; Perkins, C.; O'Brien, W.;
Cederbaum, S.; Christianson, D.W.; Zimmermann, N.; Rothenberg, M.E.; Finkelman, F.D.
"Arginase I Suppresses IL-12/IL-23p40 Driven Intestinal Inflammation during Acute
Schistosomiasis". J. Immunol. 2010, 184, 6438-6446.
177. Dowling, D.D.; Gattis, S.G.; Fierke, C.A.; Christianson, D.W. "Structures of Metal-
Substituted Human Histone Deacetylase 8 Provide Mechanistic Inferences on Biological
Function". Biochemistry 2010, 49, 5048-5056.
178. Dowling, D.D.; Ilies, M.; Olszewski, K.L.; Portugal, S.; Mota, M.M.; Llinás, M.;
Christianson, D.W. "Crystal Structure of Arginase from Plasmodium falciparum and
Implications for L-Arginine Depletion in Malarial Infection. Biochemistry 2010, 49, 5600-5608.
179. Smith, A.B., III.; Xiong, H.; Charnley, A.K.; Brenner, M.; Mesaros, E.F.; Kenesky, C.S.;
Di Costanzo, L.; Christianson, D.W.; Hirschmann, R. "Design, Synthesis and Structural Analysis
of D,L-Mixed Polypyrrolinones 2: Macrocyclic Hexapyrrolinones". Org. Lett. 2010, 12, 2994-
2997.
180. Aaron, J.A.; Christianson, D.W. "Trinuclear Metal Clusters in Catalysis by Terpenoid
Synthases". Pure Appl. Chem. 2010, 82, 1585-1597.
181. Köksal, M.; Zimmer, I.; Schnitzler, J.-P.; Christianson, D.W. "Structure of Isoprene
Synthase Illuminates the Chemical Mechanism of Teragram Atmospheric Carbon Emission". J.
Mol. Biol. 2010, 402, 363-373 (cover article).
182. Köksal, M.; Jin, Y.; Coates, R.M.; Croteau, R.; Christianson, D.W. "Taxadiene Synthase
Structure and Evolution of Modular Architecture in Terpene Biosynthesis". Nature 2011, 469,
116-120. Advanced Online Publication, 15 December 2010, doi:10.1038/nature09628.
183. Cole, K.E.; Gattis, S.G.; Angell, H.D.; Fierke, C.A.; Christianson, D.W. "Structure of the
Metal-Dependent Deacetylase LpxC from Yersinia enterocolitica Complexed with the Potent
Inhibitor CHIR-090". Biochemistry 2011, 50, 258-265.
184. Lombardi, P.M.; Angell, H.D.; Whittington, D.A.; Flynn, E.F.; Rajashankar, K.R.;
Christianson, D.W. "Structure of Prokaryotic Polyamine Deacetylase Reveals Evolutionary
Functional Relationships with Eukaryotic Histone Deacetylases". Biochemistry 2011, 50, 1808-
1817.
185. Köksal, M.; Hu, H.; Coates, R.M.; Peters, R.J.; Christianson, D.W. "Structure and
Mechanism of the Diterpene Cyclase ent-Copalyl Diphosphate Synthase". Nature Chem. Biol.
2011, 7, 431-433.
186. Cole, K.E.; Dowling, D.P.; Boone, M.A.; Phillips, A.J.; Christianson, D.W. "Ideal
Thiolate-Zinc Coordination Geometry in Depsipeptide Binding to Histone Deacetylase 8". J.
Am. Chem. Soc. 2011, 133, in press (Communication to the Editor).
187. Ilies, M.; Di Costanzo, L.; Dowling, D.P.; Thorn, K.J.; Christianson, D.W. "Binding of
α,α-Disubstituted Amino Acids to Arginase Suggests New Avenues for Inhibitor Design". J.
Med. Chem. 2011, 54, in press.
16

DAVID WILLIAM CHRISTIANSON
188. Ilies, M.; Dowling, D.P.; Lombardi, P.M.; Christianson, D.W. "Synthesis of a New
Trifluoromethylketone Analogue of L-Arginine and Contrasting Inhibitory Activity against
Human Arginase I and Histone Deacetylase 8". Bioorg. Med. Chem. Lett., in press.
189. Lombardi, P.M.; Cole, K.A.; Dowling, D.P.; Christianson, D.W. "Structure, Mechanism,
and Inhibition of Histone Deacetylases and Related Metalloenzymes". Curr. Op. Struct. Biol.
2011, 21, in press (invited review).
190. Deardoff, M.A.; Bando, M.; Saitoh, K.; Itoh, T.; Katou, Y.; Minamino, M.; Kaur, M.;
Francey, L.; Wilde, J.J.; Ernst, S.; Clark, D.; Cole, K.E.; Lombardi, P.M.; Takagaki, K.; Hirota,
T.; Nozaki, N.; Willems, P.; Gyftodimou, G.; Petersen, M.B.; Tyshchenko, N.; De Baere, E.;
Mortier, G.R.; Siu, V.M.; Gillessen-Kaesbach, G.; Christianson, D.W.; Kaiser, F.J.; Jackson,
L.G.; Krantz, I.D.; Shirahige, K. "HDAC8 Mutations in Cornelia de Lange Syndrome Provide
Insight into the Cohesin Acetylation Cycle". Nature, submitted.
191. D'Antonio, E.L.; Christianson, D.W. "Crystal Structures of Complexes with Cobalt-
Reconstituted Human Arginase I". Biochemistry, submitted.
17

Scientific Journals:
DAVID WILLIAM CHRISTIANSON
RESEARCH COMMENTS AND HIGHLIGHTS
"Creating Isoprenoid Diversity", by James C. Sacchettini and C. Dale Poulter. Science 1997,
277, 1788-1789.
"First Look at Key Enzyme Structures: Three Terpenoid Cyclases Offer Clues to Some of
Biology's Most Complex Chemistry", by Rebecca Rawls. Chemical & Engineering News 1997,
75, 7.
"Structure Sheds Light on Evolution of Biocatalysis", by Rebecca Rawls. Chemical &
Engineering News 1998, 76, 32-33.
"Protective Enzyme Yields to Structural Analysis", by Stu Borman. Chemical & Engineering
News 1999, 77, 9-10.
"Arginase Inhibitors Could be New Drugs for Impotence", by Elizabeth Wilson. Chemical &
Engineering News 1999, 77, 76-78.
"Molecular Scaffolds for Chemical Wizardry: Learning Nature's Rules for Terpene Cyclases", by
Bryan Greenhagen and Joseph Chappell. Proceedings of the National Academy of Sciences
USA 2001, 98, 13479-13481.
"Beyond Viagra: Arginase Inhibitors Touted as Potential Drug Target for Sexual Dysfunction",
by Elizabeth Wilson. Chemical & Engineering News 2003, 81, 9.
"A Step in Bile Acid Biosynthesis". Journal of Biological Chemistry 2008, 283, e99925.
"Arginine and Arginase: Endothelial NO Synthase Double Crossed?", by Paul M. Vanhoutte.
Circulation Research 2008, 102, 866-868.
"Arterial Aging is Risky", by Edward G. Lakatta. Journal of Applied Physiology 2008, 105,
1321-1322.
"Enzyme's Dual Nature Revealed", by Stu Borman. Chemical & Engineering News 2010, 88, 9.
"Dissecting a Diterpenoid Cyclase", by Eva J. Gordon. ACS Chemical Biology 2011, 6, 126.
"Taxing Enzyme: Crystallography Reveals Biomolecular Link", by David Bradley.
SpectroscopyNOW.com, 5 January 2011.
18

Business Media:
DAVID WILLIAM CHRISTIANSON
RESEARCH COMMENTS AND HIGHLIGHTS
Arginetix, Inc. Completes $2.3 Million Initial Financing and Technology License".
Fiercebiotech.com, 23 October 2008. Web archive: http://www.fiercebiotech.com/pressreleases/arginetix-inc-completes-2-3-million-initial-financing-and-technology-license.
"Arginetix Advances Preclinical Arginase Inhibitors for PAH, ED", by Trista Morrison.
BioWorld Today, 12 February 2009, page 1.
"Arginetix Closes $10.75 Million Series A Financing", by Kristy DelMuto. BioSpace.com, 2
October 2009. Web archive: http://www.biospace.com/news_story.aspx?NewsEntityId=157872.
"Baltimore's Arginetix Merges with Immune Control Inc. to form Corridor Pharmaceuticals" by
Gus Sentementes. Baltimore Sun, 17 June 2010. Web archive:
http://weblogs.baltimoresun.com/news/technology/2010/06/maryland_biotech_merger.html.
"Immune Control Taken Over by Baltimore Firm in $15M Deal", by Joseph DiStefano.
Philadelphia Inquirer, 17 June 2010. Web archive: http://www.philly.com/philly/blogs/inqphillydeals/Immune_Control_taken_over_by_Baltimore_firm_in_15M_deal.html.
19

Television:
DAVID WILLIAM CHRISTIANSON
RESEARCH HIGHLIGHTS IN THE GENERAL MEDIA
"Study Suggests New Therapy for Impotence", by Holly Firfer. CNN, air date 27 October 1999.
Web archive: http://www.cnn.com/HEALTH/men/9910/27/erection.drug/index.html.
"New Target for Treating Female Sexual Dysfunction" WB Television Network (Philadelphia
Channel 17), various air dates, summer 2003.
Print:
"N. Attleboro Senior is Energy Saver", by Phyllis Coons. Boston Globe, 10 April 1979, page 32.
"North Man Standout Student at Harvard", by Scott James. Sun Chronicle (Attleboro, MA), 21
June 1983, page 2.
"Beginning Scientists Face a Research Fund Drought", by Gina Kolata. New York Times, 5
June 1990, page C1.
"Viagra No. 2 On the Way". Metro London, 28 October 1999, page 1.
"A Better Viagra?", by Sono Motoyama. Pennsylvania Current, 2 December 1999, page 2.
"Enzyme Targeted in Effort to Prevent Impotence". Philadelphia Inquirer, 1 November 1999,
Health and Science Section, page E5.
"Ailment: You Can't Get It Up". Men's Health, September 2000.
"Lost Libido? Scientists Found It", by John F. Morrison. Philadelphia Daily News, 23 February
2001, page 12.
"New Hope for Sexual Dysfunction Cited", by Bret Hays. Daily Pennsylvanian, 9 March 2001.
"The Reason Your Sex Life Is Dull". Men's Health, August 2001.
20

Current Grant Support:
DAVID WILLIAM CHRISTIANSON
EXTRAMURAL FUNDING
"Engineering Manganese Metalloenzymes", NIH GM49758, $1,267,234 (total costs), 2/1/10 -
1/31/14.
"Structure and Function of Biosynthetic Enzymes", NIH GM56838, $1,267,234 (total costs),
8/1/10 - 7/31/14.
Pending Grant Support:
"Structure-Based Design of 129 Xe NMR Biosensors for Multiplexed Cancer Detection", NIH
GM97478, $3,021,790 (total costs), 7/1/11 - 6/30/16.
"Structure and Mechanism of Isoprene Synthase, a Non-Petrochemical Source of Carbon
Material Precursors", Department of Energy, $600,000 total costs, 7/1/11 - 6/30/14.
Completed Grant Support (last 5 years):
"Structure/Function of Epoxide Hydrolase", NIH GM63106, $777,433 (total costs), 7/1/02 -
6/30/06.
"Structural and Chemical Biology of Arginase in Asthma", Sandler Program for Asthma
Research, $750,000 (total costs), 7/1/06 - 6/30/10.
21

Invited Lectures:
DAVID WILLIAM CHRISTIANSON
DuPont Central Research, 1988
Bryn Mawr College, Department of Chemistry, 1988
New Jersey Institute of Technology, Department of Chemistry, 1988
North Attleboro High School (commencement speaker), 1989
Pennsylvania State University, Department of Chemistry, 1989
Squibb, 1989
MIT, Department of Chemistry, 1989
Indiana University, Department of Chemistry, 1990
Fels Institute, Temple University, 1990
Albert Einstein College of Medicine, Department of Biochemistry, 1990
Ursinus College, Department of Chemistry, 1990
Johns Hopkins University, Department of Biophysics and Biophysical Chemistry, 1990
The Scripps Research Institute, Department of Molecular Biology, 1991
Merck Research Laboratories, West Point, 1991
NIH, 1991
Alcon Laboratories, Inc., 1992
Temple University School of Medicine, Department of Biochemistry, 1992
Syntex (Toronto), 1992
Center for Advanced Research on Biotechnology (University of Maryland /NIST), 1992
Princeton University, Department of Chemistry, 1992
Millersville University, Department of Chemistry, 1992
Texas A & M University, Department of Chemistry, 1992
University of Lund, Department of Structural Chemistry, 1992
Institute for Cancer Research, Fox Chase Cancer Center, 1993
Wistar Institute, 1993
Fels Institute, Temple University, 1993
DNX, Inc., 1993
Boston University, Department of Biophysics, 1993
University of Florida, Department of Biochemistry, 1993
Max Planck Institut für Biochemie, 1993
Brown University, Section of Molecular Pharmacology, 1993
Brown University, Department of Chemistry, 1993
University of Texas Southwestern Medical Center, Department of Biochemistry, 1993
St. Louis University Medical Center, Doisey Department of Biochemistry, 1993
University of Maryland School of Medicine, Department of Biochemistry, 1993
Texas A & M University, Department of Biochemistry, 1994
Alcon Laboratories, Inc., 1994
Temple University Thrombosis Center, 1994
University of Florida, Department of Immunology and Medical Microbiology, 1994
University of Cambridge/Medical Research Council-Laboratory of Molecular Biology, 1994
Zeneca Pharmaceuticals Group, Inc. (UK), 1994
Syntex (Palo Alto), 1994
Brown University, Department of Molecular Pharmacology and Biotechnology, 1994
Yale University, Department of Molecular Biophysics and Biochemistry, 1995
University College (London), 1995
Lexin Pharmaceutical Corporation, 1995
Bristol-Myers Squibb, 1995
Brandeis University, Department of Biochemistry, 1995
George Washington University, Department of Chemistry, 1995
22

Invited Lectures (continued):
DAVID WILLIAM CHRISTIANSON
Medical University of South Carolina, College of Graduate Studies (Dean's Seminar), 1995
Albright College, Department of Chemistry, 1996
Brown University, Dept. of Molecular Pharmacology, Physiology, and Biotechnology, 1996
MIT, Department of Chemistry, 1996
Max Planck Institut für Biochemie, 1996
University of Florence, Department of Chemistry, 1996
Columbia University, Department of Chemistry, 1996
University of Cambridge/Medical Research Council-Laboratory of Molecular Biology, 1996
University of Athens, Division of Biochemistry and Molecular Biology, 1996
Hellenic Pasteur Institute (Athens), 1996
Boston University, Department of Physiology, 1996
SmithKline Beecham, 1997
Columbia University, Department of Biochemistry and Molecular Biophysics, 1997
Wyeth-Aerst, 1997
Bryn Mawr College, Department of Chemistry, 1997
Pennsylvania State University, Department of Chemistry, 1997
University of California, San Francisco, Department of Pharmaceutical Chemistry, 1997
Washington State University, Institute for Biological Chemistry, 1997
University of Utah, Department of Chemistry, 1997
Max Planck Institut für Biochemie, 1997
Duke University, Department of Biochemistry, 1997
The Scripps Research Institute, Department of Chemistry, 1997
University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, 1998
Schering-Plough Research Institute, 1998
The Scripps Research Institute, Department of Chemistry, 1998
NitroMed, Inc., 1999
ICOS, Inc., 1999
University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, 1999
University of Pennsylvania School of Medicine, Department of Physiology, 1999
University of Delaware, Department of Chemistry and Biochemistry, 1999
Phylos, Inc., 1999
Harvard University, Department of Chemistry and Chemical Biology, 2000
Yale University, Department of Molecular Biophysics and Biochemistry, 2000
Brandeis University, Department of Biochemistry, 2000
Rockefeller University, 2000
University of Massachusetts Medical School, Department of Biochemistry, 2000
Alcon Laboratories, Inc., 2000
University of California, Davis, Department of Biochemistry, 2000
University of Maryland, College Park, Department of Chemistry and Biochemistry, 2000
College of New Jersey, Department of Chemistry, 2000
Université Paris V, Laboratoire de Chimie et Biochimie, 2000
City University of New York, Department of Biochemistry, 2001
Columbia University, Department of Biochemistry and Molecular Biophysics, 2001
MCP-Hahnemann University, Department of Biochemistry, 2001
Boston University Medical Center, Department of Biochemistry, 2002
University of Lund, Center for Chemistry and Chemical Engineering, 2002
Suntory Pharmaceutical Research Laboratories, 2002
Concurrent Pharmaceuticals, 2002
University of Massachusetts-Amherst, Department of Molecular and Cellular Biology, 2002
23

Invited Lectures (continued):
DAVID WILLIAM CHRISTIANSON
Alcon Laboratories, Inc., 2003
Ursinus College, Departments of Biology and Chemistry, 2003
Concurrent Pharmaceuticals, 2003
University of Cambridge/Cambridge Institute for Medical Research, 2004
Pfizer, 2004
Shippensburg University, 2004
University of Michigan, Department of Chemistry, 2004
Duke University Medical Center, Department of Biochemistry, 2005
UNC School of Medicine, Department of Biochemistry and Biophysics, 2005
National Hellenic Research Foundation, Athens, 2005
University of Crete, Institute of Molecular Biology and Biotechnology, 2005
Johnson & Johnson, 2005
Delaware State University, Department of Chemistry, 2006
University of Cambridge, Department of Biochemistry, 2006
University of Cambridge, Department of Chemistry, 2006
University of Durham, Department of Chemistry, 2006
University of Leicester, Department of Biochemistry, 2006
Université René Descartes–Paris V, Laboratoire de Chimie et Biochimie, 2006
Johns Hopkins University, School of Medicine, 2007
Cardiff University, School of Chemistry, 2007
University of Siena, Biostructure Laboratory, 2007
University of Florence, Department of Chemistry, 2007
University of California, Davis, Department of Chemistry, 2007
Achaogen, 2007
Institutes for Pharmaceutical Discovery, 2008
University of Pennsylvania, School of Medicine, 2009
Johns Hopkins University, Department of Chemistry, 2010
University of California, Los Angeles, Department of Chemistry, 2010
University of California, Los Angeles, Department of Genetics, 2010
University of Athens, School of Pharmacy, 2010
Firmenich, 2010
Corridor Pharmaceuticals, 2010
University of Chicago, Department of Chemistry, 2011
University of Illinois, Urbana-Champaign, Department of Biochemistry, 2011
University of Maryland, School of Pharmacy, 2011
Iowa State University, Department of Biochemistry, 2011
Boston University, Department of Physiology and Biophysics, 2011
Drexel University, Department of Chemistry, 2011
Colgate University, Department of Chemistry, 2011
Hauptman-Woodward Institute, 2012
24

Invited Symposia Lectures:
DAVID WILLIAM CHRISTIANSON
39th Mosbacher Colloqium, Mosbach, West Germany, 1988
Protein Society, Third Annual Meeting, Seattle, WA, 1989
Delaware Valley Enzymology Club/ACS Regional Meeting, Philadelphia, PA, 1989
ONR/FASEB Molecular Recognition Meeting, Charleston, SC, 1990
ACS Medicinal Chemistry Symposium, Buffalo, NY, 1990
CO 2 , HCO 3 - H + , and the Carbonic Anhydrases, Hannover, Germany, 1991
Structure and Function of Mutant Proteins, Firenze, Italy, 1991
ONR/FASEB Metal Ion Biosensor Meeting, Washington, D.C., 1991
Gordon Conference on Metals in Biology, Ventura, CA, 1992
Searle Scholars Meeting, Chicago, IL, 1992
Design of Metal Binding Peptides and Proteins (ACS, Inorganic Division), San Francisco, CA, 1992
Penn State Summer Symposium on Molecular Biology, University Park, PA, 1993
Keystone Symposium on Protease Function and Inhibition, Santa Fe, NM, 1994
Franklin Institute Symposium on Deciphering Molecules and Advancing Health, Philadelphia, PA, 1994
Gordon Conference on Diffraction Methods, Concord, NH, 1994
Gordon Conference on Proteolytic Enzymes and their Inhibitors, New London, NH, 1994
ACS Symposium on Computer-Assisted Drug Design, Fort Worth, TX, 1994
Josef Fried Symposium of Bioorganic Chemistry, University of Chicago, IL, 1994
ONR/FASEB Biomolecular Recognition Meeting, Berkeley Springs, WV, 1994
4th International Conference on the Carbonic Anhydrases, Oxford, United Kingdom, 1995
Gordon Conference on Enzymes, Coenzymes, and Metabolic Pathways, Meriden, NH, 1995
34th Hanford Symposium on Health and the Environment, Pasco, WA, 1995
University of Pennsylvania Enzyme Symposium II, Philadelphia, PA, 1996
ONR/FASEB Biomolecular Recognition Meeting, Berkeley Springs, WV, 1996
Retreat on Chemistry in Medicine, University of Pennsylvania, PA, 1996
Gordon Conference on Enzymes, Coenzymes, and Metabolic Pathways, Meriden, NH, 1997
Cambridge Healthtech Institute Conference on Inhibition of Human Proteases, Boston, MA, 1998
Pfizer Award Symposium Lecture, ACS Division of Biological Chemistry, San Francisco, CA, 1999
Frontiers in Biomedical Research Symposium, Indian Wells, CA, 1999
Symposium on Frontiers in Biomedical Research, Philadelphia, PA, 2000
Welch Conference on Macromolecular Structures and Function, Houston, TX, 2000
5 th Annual Structural Biology Symposium, University of Texas Medical Branch, Galveston, TX, 2000
American Crystallographic Association Annual Meeting, St. Paul, MN, 2000
17 th Enzyme Mechanisms Conference, Marco Island, FL, 2001
Repligen Award Symposium in honor of Dale Poulter (ACS Biological Division), Boston, MA, 2002
Metalloenzyme Structure and Mechanism (ACS Biological Division; organizer), New York, NY, 2003
Wyeth Symposium on Female Sexual Dysfunction, Collegeville, PA, 2003
Delaware Valley Enzymology Club, Haverford College, 2004
American Society for Microbiology ICAAC Meeting, Washington, D.C., 2004
International Symposium on Receptors of the Immune System, Barcelona, Spain, 2007
Annual Meeting, Sandler Program for Asthma Research, San Francisco, CA, 2007
236 th National Meeting, American Chemical Society, Philadelphia, PA, 2008
Chemistry of the Cell Symposium (keynote address), 42 nd IUPAC/RSC Congress, Glasgow, Scotland,
2009
8 th International Conference on the Carbonic Anhydrases, Florence, Italy, 2009
American Asthma Foundation, San Francisco, CA, 2009
Gordon Conference on Polyamines, Waterville Valley, NH, 2011
TERPNET 2011, 10 th International Meeting on Isoprenoids, Kalmar, Sweden, 2011
25

Research Personnel:
Postdoctoral Fellows:
DAVID WILLIAM CHRISTIANSON
Anzhi Wei (1989 - 1994). Diploma, Quinghau University, 1970 (Bejing, China). Current
position: Research Scientist, Bristol-Meyers Squibb, Princeton, NJ.
Zdzislaw Wawrzak (1994 - 1995). Ph.D., Technical University of Lodz (Lodz, Poland), 1988.
Current position: APS Research Scientist; Research Associate Professor, Northwestern
University, Evanston, IL.
Daniel Elbaum (1994 - 1996). Ph.D., Harvard University, 1994 (Cambridge, MA). Current
Position: Director, Discovery Chemistry, FoldRx Pharmaceuticals, Inc., Cambridge, MA.
Robert Heck (1995 - 1997). Ph.D., University of Florida, 1995 (Gainesville, FL). Current
Position: Senior Research Scientist, Pacific Northwest National Laboratory, Pasco, WA.
Rick Baggio (1996 - 1998). Ph.D., Brandeis University, 1996 (Waltham, MA). Current
Position: Senior Scientist, Phylos, Lexington, MA.
Michael Rynkiewicz (1999 - 2001). Ph.D., Boston University, 1999 (Boston, MA). Current
Position: Senior Scientist, Suntory Pharmaceutical Research Laboratories, Cambridge, MA.
J. David Cox (2000 - 2002). Ph.D., University of Pennsylvania, 2000 (Philadelphia, PA).
Current Position: Director, US Medical Affairs, Esai Inc., Woodcliff Lake, NJ.
Douglas Whittington (2000 - 2003). Ph.D., Massachusetts Institute of Technology, 2000
(Cambridge, MA). Current Position: Senior Scientist, Amgen, Cambridge, MA.
Hyunshun Shin (2001 - 2005). Ph.D., Drexel University, 2001 (Philadelphia, PA). Visiting
Assistant Professor, Philadelphia University, 2005-2006; Assistant Professor of Chemistry,
University of San Francisco, 2006-2008; Current Position: Assistant Professor Chemistry,
McMurray University, Abilene, TX.
Luigi Di Costanzo (2003 - 2009). Ph.D., University of Trieste, 2003 (Italy). Current Position:
Staff Scientist, Research Collaboratory for Structural Bioinformatics, New Brunswick, NJ.
Monica Ilies (2006 - 2010). Ph.D., Polytechnic University of Bucharest, 2002. Current Position:
Assistant Professor, Drexel University, Philadelphia, PA.
Tatiana Zahkarian (2006-2009). Ph.D., Rice University, 2005. Current Position: Research
Scientist, Novartis.
Kathryn Cole (2009 - 2011). Ph.D., Yale University, 2009. Future Position: Assistant Professor
of Chemistry, Ithaca College (July 2011).
Mustafa Köksal (2007 - present). Ph.D., Purdue University, 2007.
Edward D'Antonio (2010 - present). Ph.D., North Carolina State University, 2010.
26

Research Personnel:
Ph.D. Students:
DAVID WILLIAM CHRISTIANSON
Richard S. Alexander. B.S., Philadelphia College of Textiles and Science, 1987; Ph.D.,
University of Pennsylvania, 1992. Dissertation: "Structural Studies of Human Carbonic
Anhydrase II". Current position: Senior Research Scientist, Johnson & Johnson, Exton, PA.
Satish K. Nair. Sc.B., Brown University, 1989; Ph.D., University of Pennsylvania, 1994.
Dissertation: "Structural Aspects of the Carbonic Anhydrase Mechanism". Leukemia Society
Postdoctoral Fellow, Rockefeller University (with S.K. Burley). Current position: Associate
Professor of Biochemistry and Chemistry (with tenure), University of Illinois, Urbana-
Champaign.
Joseph A. Ippolito. B.A., University of Pennsylvania, 1990; Ph.D., University of Pennsylvania,
1995. Dissertation: "Structure-Assisted Redesign of the Zinc Binding Site in Human Carbonic
Anhydrase II". NIH Postdoctoral Fellow, Yale University (with T.A. Steitz). Current position:
Senior Scientist, Rib-X Pharmaceuticals, New Haven, CT.
Darin S. Katz. B.S., Pennsylvania State University, 1991; Ph.D., University of Pennsylvania,
1995. Dissertation: "Structural Studies of Protein-Protein Interactions". NIH Postdoctoral
Fellow, Fox Chase Cancer Center (with J. Feng). Current position: Science Department
Chairman, Agnes Irwin School.
Zoltan F. Kanyo. B.S., Ohio State University, 1988; Ph.D., University of Pennsylvania, 1996.
Dissertation: "The Structure of Rat Liver Arginase". Postdoctoral Fellow, University of
California at San Francisco (with S. Pruisner and R.J. Fletterick). Current Position: Senior
Scientist, Rib-X Pharmaceuticals, New Haven, CT.
P. Ann Boriack-Sjodin. B.S., Harvey Mudd College, 1992. Ph.D., University of Pennsylvania,
1996. Dissertation: "Structure and Mechanism of Mitochondrial Carbonic Anhydrase V".
Postdoctoral Fellow, Rockefeller University, 1996-1998 (with J. Kuriyan). Current position:
Director, Structural Biology, AstraZeneca Pharmaceuticals, Waltham, MA.
Laura R. Scolnick. B.S., Massachusetts Institute of Technology, 1992. Ph.D., University of
Pennsylvania, 1997. Dissertation: "Tale of Two Metalloenzymes: Structure-Function
Analogies between Carbonic Anhydrase and Arginase". Postdoctoral Fellow, University of
California, Los Angeles (with E. Neufeld and D. Eisenberg). Current position: Freelance
Science Writer, Boston, MA.
Charles A. Lesburg. A.B., Harvard College, 1992. Ph.D., University of Pennsylvania, 1997.
Dissertation: "Metalloenzyme Structure and Function". Current Position: Senior Scientist,
Schering-Plough.
Christine M. Lukacs. A.B., Harvard College, 1992. Ph.D., University of Pennsylvania, 1997.
Dissertation: "Studies of Beta-Sheet Rearrangements in Antichymotrypsin". Damon-Runyon
Postdoctoral Fellow, Mount Siani Hospital (with A. Aggarwal). Current position: Principal
Scientist, Hoffman-LaRoche, Nutley, NJ.
27

Research Personnel:
Ph.D. Students (continued):
DAVID WILLIAM CHRISTIANSON
Travis M. Stams. B.S., University of Rochester, 1991. Ph.D., University of Pennsylvania,
1998. Dissertation: "Structure-Function Relationships in Metalloenzymes: Carbonic Anhydrase
IV and Ribonuclease P". Current Position: Director of Biomolecular Structure, Novartis
Institutes for Biomedical Research, Cambridge, MA.
Maria Argiriadi. B.A., College of William and Mary, 1994; M.A., College of William and
Mary, 1994. Ph.D., University of Pennsylvania, 1999. Dissertation: "Structure-Based
Mechanism and Evolution of Liver Epoxide Hydrolase" (John G. Miller Award for Most
Outstanding Ph.D. Thesis, 1999). Postdoctoral Fellow, Rockefeller University (with J. Kuriyan).
Current position: Senior Scientist, Abbot Laboratories, Worcester, MA.
Jonathan M. Caruthers. B.S., University of Colorado, 1996; Ph.D., University of
Pennsylvania, 2000. Dissertation: " Aristolochene Synthase Structure and Function".
Postdoctoral Fellow, Stanford University (with D. McKay). Current position: Staff Scientist,
Stanford Linear Accelerator.
J. David Cox. B.A., Rutgers University, 1996; Ph.D., University of Pennsylvania, 2000.
Dissertation: "Arginase: Structure-Based Mechanism and Role in Erectile Function" (John G.
Miller Award for Most Outstanding Ph.D. Thesis, 2000). Postdoctoral Fellow, University of
Pennsylvania (with D. Christianson). Scientific Associate, Precept Medical Communications,
Short Hills, NJ; Manager, Hypertension Medical Strategy, Bristol-Myers Squibb; Current
Position: Associate Medical Director, Esai Inc., Woodcliff Lake, NJ.
Chiana Paschall. B.S., University of Maryland, Baltimore County, 1996; Ph.D., University of
Pennsylvania, 2000. Dissertation: "Polyene Cyclization Reactions". Current position: Real
estate entrepreneur.
Chu-Young Kim. B.A., Cornell University, 1996; Ph.D., University of Pennsylvania, 2001.
Dissertation: "Structural Investigation of Inhibitor Binding to Carbonic Anhydrases II and IV
Using Crystallographic and Computational Methods". Postdoctoral Fellow, Stanford University
(with C. Khosla). Current Position: Assistant Professor of Biological Sciences, National
University of Singapore.
Evis Cama. B.A., Macallaster College, 1998. Ph.D., University of Pennsylvania, 2003.
Dissertation: "Structure Determination of Human Arginase II" (John G. Miller Award for Most
Outstanding Ph.D. Thesis, 2003). Postdoctoral Fellow, University of Cambridge (with J.
Huntington). Current position: Analyst, L.E.K. Consulting, Ltd. (Knightsbridge, London).
German Gomez. B.A., Albright College, 2000. Ph.D., University of Pennsylvania, 2006.
Dissertation: "Bifunctional Catalysis in Human Epoxide Hydrolase". Current position:
Scientist, Locus Pharmaceuticals.
Kevin Jude. B.S., Georgia Tech, 1999. Ph.D., University of Pennsylvania, 2007. Dissertation:
"Crystallography at Ultrahigh and Infralow Resolution: Studies of Carbonic Anhydrase and
Ribonuclease P". Current position: Postdoctoral Fellow, University of California, Berkeley
(with J. Berger).
28

Research Personnel:
Ph.D. Students (continued):
DAVID WILLIAM CHRISTIANSON
L. Sangeetha Vedula. B.Sc., Indian Institute of Technology, 2001. Ph.D., University of
Pennsylvania, 2007. Dissertation: "Sesquiterpene Biosynthesis". Current position:
Postdoctoral Fellow, Drexel University College of Medicine (with P. Loll).
Ekaterina Shishova. B.A., Mount Holyoke College, 2001; M.S., Stanford University, 2003.
Ph.D., University of Pennsylvania, 2007. Dissertation: "Structure-Function Correlations in
Metalloenzymes: Aristolochene Synthase and Arginase I". Current position: Postdoctoral
Fellow, Harvard University (with J. Cohen).
Heather Gennadios. B.S., UNC Chapel Hill, 2000. Ph.D., University of Pennsylvania, 2008.
Dissertation: "Structural Studies of a Zinc-Dependent Deacetylase and a Sesquiterpene
Cyclase". Current position: Staff Scientist, Food and Drug Administration.
Daniel Dowling. B.S., Holy Cross College, 2004. Ph.D., University of Pennsylvania, 2009.
Dissertation: "Structure-Mechanism Relationships in Histone Deacetylase-8 and P. falciparum
Arginase". Current Position: Postdoctoral Fellow, MIT (with Cathy Drennan).
Julie Aaron. B.S., McMaster University, 2005. Ph.D., University of Pennsylvania, 2010.
Dissertation: "Structure and Mechanism of Epi-Isozizaene Synthase". Current Position:
Assistant Professor of Chemistry, DeSales University.
Patrick Lombardi. B.S., Yale University, 2006. Ph.D., University of Pennsylvania, 2011.
Dissertation: "Structural Studies of Acetylpolyamine Amidohydrolase". Current Position:
Postdoctoral Fellow, Yale University (with A. M. Pyle).
M.S. Students:
Ann M. Cappalonga Bunn. B.S. 1990, Boston College; M.S. 1993, University of
Pennsylvania.
Vicky Chen. B.S., Kings College, 1995. M.S. 1997, University of Pennsylvania.
Kate Thorn. B.S., Haverford College, 2005. M.S. 2010, University of Pennsylvania.
29

Teaching:
DAVID WILLIAM CHRISTIANSON
Chemistry 251, Introduction to Biological Chemistry: Fall semester 1988 - 1990, 1994; Spring
semester 1991 - 1999; Summer session I, 1993.
Chemistry 557, Macromolecular Catalysis: Spring semester 1989 - 1991, 1993 - 1994, 1996 -
1997, 2003 - 2012; Fall semester 1995, 1997 - 2001.
Chemistry 451, Biochemistry: Fall semester 1991 - 1995, 1998 - 2005, 2007 - 2011.
Biochemistry 404, Senior Research Tutorial: Fall semester 1991, 1993; Spring semester 1992,
1994 – 2007.
Chemistry 1, Freshman Chemistry: Recitation, Fall semester 1992.
Departmental Committees:
Organic Faculty Search Committee, 1989 - 1990.
Graduate Academic Committee, 1989 – 1996; 2009 - .
Graduate Admissions Committee, 1989 – 1996; 2011 - .
Seminar Committee, 1989 - 1996; (Chairman, 1992 - 1996).
Committee to Revise Freshman Chemistry, 1991 - 1993.
Five-Year Planning Committee, 1992 - 1993; 1998 – 2000; 2003 – 2004; 2007 - 2008.
Adjunct Faculty Appointment Committee (Chairman), 1994.
Biological Faculty Search Committee, 1994 - 1996; 2000-2002, (Chairman 1996, 2001 - 2002).
Junior Faculty Search Committees, 1997 - 1998, 2003-2009 (Chairman 2005 - 2009).
Undergraduate Awards Committee, 1994 - 1996.
Chair's Advisory Committee, 2002 - 2010.
School and University Committees:
Board of Freshman Advisers, 1989 - 1993.
Core Science Curriculum Committee, 1989 - 1990.
SAS Curriculum Committee, 1991 - 1993.
Curtis Organ Restoration Society, 1993.
Biophysics Faculty Search Committee, 1994 - 1995.
SAS Task Force on M.S. Program in Biotechnology, 1996 - 1997.
Provost's Conflict of Interest Standing Committee, 1998 - 2000.
SAS Planning and Priorities Committee (advisory to the Dean), 2000 - 2003.
SAS Personnel Committee (promotions and tenure), 2000 - 2001.
Hearing Board, Student Disciplinary Committee (academic dishonesty), 2005 - 2008.
Other Service:
Membership Committee (Chairman), The Protein Society, 1996 - 1998.
Editorial Advisory Board, Protein Science, 2004 - 2010.
Councilor, Division of Biological Chemistry, American Chemical Society, 2001 - 2002.
Co-Organizer, University of Pennsylvania Enzyme Symposium, 1995 - 1998.
Structural Biology Advisory Committee, Medical University of South Carolina, 1995 - 2000.
Tenure Review ("Ad Hoc") Committee, Faculty of Arts and Sciences, Harvard University, 2004.
Alumni interviewer, Harvard Admissions Office, 1998 - .
Instrument Advisory Team, High Flux Isotope Reactor, Oak Ridge National Laboratory, 2007 - .
National Academies Board on Chemical Sciences and Technology, 2011 - 2014.
30